Results 91 to 100 of about 323,472 (305)

Diversity and complexity in neural organoids

FEBS Letters, EarlyView.
Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley   +1 more source

Guiding Bacteria with Small Molecules and RNA [PDF]

Journal of the American Chemical Society, 2007
Chemotactic bacteria navigate their chemical environment by coupling sophisticated information processing capabilities to molecular motors that propel the cells forward. The ability to reprogram bacteria to follow entirely new chemical signals would create powerful new opportunities in bioremediation, bionanotechnology, and synthetic biology.
Shana, Topp, Justin P, Gallivan
openaire   +2 more sources

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging

, 2019
Clustered regularly interspaced short palindromic repeats (CRISPR) guide RNA scaffolds have been adapted to carry multiple binding sites for fluorescent proteins to enhance brightness for live cell imaging of genomic loci.
Pederson, Thoru, Zhang, Shaojie, Ma, Hanhui, Chung, Yu-Chieh, Naseri, Ardalan, Tu, Li-Chun, Grunwald, David   +6 more
core   +1 more source

RNA interference-mediated co-transcriptional gene silencing in fission yeast [PDF]

, 2012
In the last decade or so, RNA interference (RNAi) has gained unanticipated recognition in the fields of RNA biology and gene regulation. It exists in a wide variety of eukaryotic organisms, and various forms of RNAi are involved in diverse biological ...
Woolcock, Katrina Jane
core   +1 more source

Linking neurogenesis, oligodendrogenesis, and myelination defects to neurodevelopmental disruption in primary mitochondrial disorders

FEBS Letters, EarlyView.
Mitochondrial remodeling shapes neural and glial lineage progression by matching metabolic supply with demand. Elevated OXPHOS supports differentiation and myelin formation, while myelin compaction lowers mitochondrial dependence, revealing mitochondria as key drivers of developmental energy adaptation.
Sahitya Ranjan Biswas, Porter L. Tomsick, Alicia M. Pickrell, Paul D. Morton   +3 more
wiley   +1 more source

Contribution of two conserved histidines to the dual activity of archaeal RNA guide-dependent and -independent pseudouridine synthase Cbf5 [PDF]

, 2015
International audienceIn all organisms, several distinct stand-alone pseudouridine synthase (PUS) family enzymes are expressed to isomerize uridine into pseudouridine (Psi) by specific recognition of RNAs.
Kothe, Ute, Fourmann, Jean-Baptiste, Charpentier, Bruno, Wieden-Kothe, Ute, Loegler, Christine, Tillault, Anne-Sophie, Wieden, Hans-Joachim   +6 more
core   +1 more source

Genome editing in maize directed by CRISPR–Cas9 ribonucleoprotein complexes

Nature Communications, 2016
Genome editing in plants typically requires the expression of Cas9 and guide RNA from stably transformed plasmid DNA. Here, the authors show that successful editing can be achieved after delivery of the Cas9-guide RNA complex as a ribonucleoprotein to ...
Sergei Svitashev, Christine Schwartz, Brian Lenderts, Joshua K. Young, A. Mark Cigan   +4 more
doaj   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source

A Step-by-Step Guide to Study Protein–RNA Interactions

CHIMIA, 2019
Protein–RNA complex formation is at the center of RNA metabolism and leads to the modulation of protein and RNA functions. We propose here a step-by-step guide to investigate these interactions including the identification of the protein and RNA
Antoine Cléry, Laurent Gillioz, Cristina K. X. Nguyen, Frédéric H.-T. Allain   +3 more
doaj   +1 more source