Results 111 to 120 of about 570,529 (349)

Internal guide RNA interactions interfere with Cas9-mediated cleavage

Nature Communications, 2016
While the CRISPR-Cas9 system has revolutionised molecular biology, it is still a mystery why not every guide RNA elicits target DNA cleavage. Here the authors show that genomic context and internal gRNA interactions can inhibit cleavage.
Summer B. Thyme, Laila Akhmetova, Tessa G. Montague, Eivind Valen, Alexander F. Schier   +4 more
doaj   +1 more source

Cytoplasmic delivery of antibodies through grafting a functional single complementarity‐determining region loop

FEBS Letters, EarlyView.
We achieved cytoplasmic delivery of non‐cell‐penetrating IgGs by grafting a single functional complementarity‐determining region 1 (CDR1) from the light chain variable region (VL) of the cell‐internalizable 3D8 antibody. The engineered IgG acquired cell‐penetrating ability while maintaining antigen affinity, highlighting CDR1 grafting as a promising ...
Yerin Jeon, Juho Choi, Youngin Roh, Eunbin Lee, Luigie Villamante, Myung‐Hee Kwon   +5 more
wiley   +1 more source

Prokaryotic Argonautes – variations on the RNA interference theme

Microbial Cell, 2014
The discovery of RNA interference (RNAi) has been a major scientific breakthrough. This RNA-guided RNA interference system plays a crucial role in a wide range of regulatory and defense mechanisms in eukaryotes.
John van der Oost, Daan C. Swarts, Matthijs M. Jore   +2 more
doaj   +1 more source

Unlocking the potential of tumor‐derived DNA in urine for cancer detection: methodological challenges and opportunities

Molecular Oncology, EarlyView.
Urine is a rich source of biomarkers for cancer detection. Tumor‐derived material is released into the bloodstream and transported to the urine. Urine can easily be collected from individuals, allowing non‐invasive cancer detection. This review discusses the rationale behind urine‐based cancer detection and its potential for cancer diagnostics ...
Birgit M. M. Wever, Renske D. M. Steenbergen   +1 more
wiley   +1 more source

Systematic identification and characterization of high efficiency Cas9 guide RNAs for therapeutic targeting of ADAR.

PLoS ONE
Therapeutic targeting of the adenosine deaminase ADAR has great potential in cancer and other indications; however, it remains unclear what approach can enable effective and selective therapeutic inhibition.
Benjamin G Gowen, Kory Melton, Weng In Leong, Prachi Khekare, Shannon McCawley, Jean Chan, Pierre Boivin, Vihasi Jani, Aaron J Cantor, Akshay Tambe, Mary Haak-Frendscho, Mary J Janatpour, Spencer C Wei   +12 more
doaj   +1 more source

Contribution of protein Gar1 to the RNA-guided and RNA-independent rRNA:Ψ-synthase activities of the archaeal Cbf5 protein

Scientific Reports, 2018
Archaeal RNA:pseudouridine-synthase (PUS) Cbf5 in complex with proteins L7Ae, Nop10 and Gar1, and guide box H/ACA sRNAs forms ribonucleoprotein (RNP) catalysts that insure the conversion of uridines into pseudouridines (Ψs) in ribosomal RNAs (rRNAs ...
Ryosuke Fujikane, Isabelle Behm-Ansmant, Anne-Sophie Tillault, Christine Loegler, Valérie Igel-Bourguignon, Evelyne Marguet, Patrick Forterre, Christiane Branlant, Yuri Motorin, Bruno Charpentier   +9 more
doaj   +1 more source

Generation of Mutant Pigs by Direct Pronuclear Microinjection of CRISPR/Cas9 Plasmid Vectors

Bio-Protocol, 2017
A set of Cas9 and single guide CRISPR RNA expression vectors was constructed. Only a very simple procedure was needed to prepare specific single-guide RNA expression vectors with high target accuracy.
Chin-kai Chuang, Ching-Fu Tu, Chien-Hong Chen   +2 more
doaj   +1 more source

Structure and Engineering of Francisella novicida Cas9 [PDF]

, 2016
Summary The RNA-guided endonuclease Cas9 cleaves double-stranded DNA targets complementary to the guide RNA and has been applied to programmable genome editing.
Adams, Anders, Briner, Chylinski, Cong, Cowtan, Crooks, Deltcheva, Deveau, Emsley, Evans, Feng Zhang, Fonfara, Garneau, Gasiunas, Hiroshi Nishimasu, Hisato Hirano, Hsu, Hsu, Izuho Hatada, Jiang, Jiang, Jinek, Jinek, Jonathan S. Gootenberg, Kabsch, Kim, Kleinstiver, Kleinstiver, Lin, Mali, Mika Kimura, Mojica, Nishimasu, Nishimasu, Omar O. Abudayyeh, Osamu Nureki, Patrick D. Hsu, Ran, Ryuichiro Ishitani, Sampson, Sternberg, Sung, Takanori Nakane, Takuro Horii, Woo, Zetsche, Zuris   +47 more
core   +1 more source

Subpar reporting of pre‐analytical variables in RNA‐focused blood plasma studies

Molecular Oncology, EarlyView.
Pre‐analytical variables strongly influence the analysis of extracellular RNA (cell‐free RNA; exRNA) derived from blood plasma. Their reporting is essential to allow interpretation and replication of results. By evaluating 200 exRNA studies, we pinpoint a lack of reporting pre‐analytical variables associated with blood collection, plasma preparation ...
Céleste Van Der Schueren, Philippe Decruyenaere, Francisco Avila Cobos, Johanna Bult, Jill Deleu, Laudonia Lidia Dipalo, Hetty Hilde Helsmoortel, Eva Hulstaert, Annelien Morlion, Elena Ramos Varas, Kathleen Schoofs, Wim Trypsteen, Eveline Vanden Eynde, Hanne Van Droogenbroeck, Kimberly Verniers, Jo Vandesompele, Anneleen Decock   +16 more
wiley   +1 more source

Evolving Boolean Networks with RNA Editing [PDF]

arXiv, 2015
The editing of transcribed RNA by other molecules such that the form of the final product differs from that specified in the corresponding DNA sequence is ubiquitous. This paper uses an abstract, tunable Boolean genetic regulatory network model to explore aspects of RNA editing.
arxiv