Results 241 to 250 of about 570,529 (349)

PathoGD: an integrative genomics approach to primer and guide RNA design for CRISPR-based diagnostics. [PDF]

Commun Biol
Low SJ, O'Neill M, Kerry WJ, Wild N, Krysiak M, Nong Y, Azzato F, Hor E, Williams L, Taiaroa G, Steinig E, Pasricha S, Williamson DA.   +12 more
europepmc   +1 more source

CRISPRi-mediated tunable control of gene expression level with engineered single-guide RNA in Escherichia coli. [PDF]

Nucleic Acids Res, 2023
Byun G, Yang J, Seo SW.
europepmc   +1 more source

Silencing suppressor activity of the Tobacco rattle virus-encoded 16-kDa protein and interference with endogenous small RNA-guided regulatory pathways

, 2008
Llúcia Martínez‐Priego, Livia Donaire, Daniel Barajas, César Llave   +3 more
openalex   +1 more source

Multidimensional OMICs reveal ARID1A orchestrated control of DNA damage, splicing, and cell cycle in normal‐like and malignant urothelial cells

Molecular Oncology, EarlyView.
Loss of the frequently mutated chromatin remodeler ARID1A, a subunit of the SWI/SNF cBAF complex, results in less open chromatin, alternative splicing, and the failure to stop cells from progressing through the cell cycle after DNA damage in bladder (cancer) cells. Created in BioRender. Epigenetic regulators, such as the SWI/SNF complex, with important
Rebecca M. Schlösser, Florian Krumbach, Eyleen Corrales, Geoffroy Andrieux, Christian Preisinger, Franziska Liss, Alexandra Golzmann, Melanie Boerries, Kerstin Becker, Ruth Knüchel, Stefan Garczyk, Bernhard Lüscher   +11 more
wiley   +1 more source

Conformational transitions of Streptococcus pyogenes Cas9 induced by salt and single-guide RNA binding. [PDF]

J Biol Chem
He Y, Zalenski N, Stephenson AA, Raper AT, Ghimire C, Suo Z.   +5 more
europepmc   +1 more source

Synthetic Gene Circuits Combining CRISPR Interference and CRISPR Activation in E. coli: Importance of Equal Guide RNA Binding Affinities to Avoid Context-Dependent Effects. [PDF]

ACS Synth Biol, 2023
Barbier I, Kusumawardhani H, Chauhan L, Harlapur PV, Jolly MK, Schaerli Y.   +5 more
europepmc   +1 more source

Enhancing specificity of RNA-guided clustered, regularly interspaced short palindromic repeats (CRISPR)-based genome editing with paired guide RNAs [PDF]

, 2013

openalex   +1 more source

Germline variants in CDKN2A wild‐type melanoma prone families

Molecular Oncology, EarlyView.
Among melanoma‐prone families, wild‐type for CDKN2A and CDK4, some have pathogenic variants in genes not usually linked to melanoma. Furthermore, rare XP‐related variants and variants in MC1R are enriched in such families. Germline pathogenic variants in CDKN2A are well established as an underlying cause of familial malignant melanoma. While pathogenic
Gjertrud T. Iversen, Marie Loeng, Amalie Lund Holth, Per E. Lønning, Jürgen Geisler, Stian Knappskog   +5 more
wiley   +1 more source

MAIGRET: a CRISPR-based immunoassay that employs antibody-induced cell-free transcription of CRISPR guide RNA strands. [PDF]

Nucleic Acids Res
Miceli F, Bracaglia S, Sorrentino D, Porchetta A, Ranallo S, Ricci F.   +5 more
europepmc   +1 more source

Binding to the conserved and stably folded guide RNA pseudoknot induces Cas12a conformational changes during ribonucleoprotein assembly. [PDF]

J Biol Chem, 2023
Sudhakar S, Barkau CL, Chilamkurthy R, Barber HM, Pater AA, Moran SD, Damha MJ, Pradeepkumar PI, Gagnon KT.   +8 more
europepmc   +1 more source