Results 241 to 250 of about 4,450,920 (398)

Structural basis for guide RNA selection by the RESC1-RESC2 complex. [PDF]

Nucleic Acids Res, 2023
Dolce LG, Nesterenko Y, Walther L, Weis F, Kowalinski E.   +4 more
europepmc   +1 more source

Peroxidasin enables melanoma immune escape by inhibiting natural killer cell cytotoxicity

Molecular Oncology, EarlyView.
Peroxidasin (PXDN) is secreted by melanoma cells and binds the NK cell receptor NKG2D, thereby suppressing NK cell activation and cytotoxicity. PXDN depletion restores NKG2D signaling and enables effective NK cell–mediated melanoma killing. These findings identify PXDN as a previously unrecognized immune evasion factor and a potential target to improve
Hsu‐Min Sung, David Bickel, Lena C. M. Krause, Daria Ezeriņa, Christian Ickes, Julian Wojtachnia, Christine S. Gibhardt, Magdalena Shumanska, Khadija Wahni, Andrea Paluschkiwitz, Julia Malo Pueyo, Ekaterina Baranova, Wim Vranken, Hedwig Stanisz, Ioana Stejerean‐Todoran, Michael P. Schön, Joris Messens, Ivan Bogeski   +17 more
wiley   +1 more source

CRISPy-web 3.0: A unified platform for multi-modal guide RNA design for CRISPR and TnpB genome editing applications. [PDF]

Synth Syst Biotechnol
Yang S, Cai Z, Chia N, Luo J, Weber T, Blin K, Tong Y.   +6 more
europepmc   +1 more source

Redox regulation meets metabolism: targeting PRDX2 to prevent hepatocellular carcinoma

Molecular Oncology, EarlyView.
PRDX2 acts as a central redox hub linking metabolic dysfunction‐associated steatohepatitis (MASH) to hepatocellular carcinoma (HCC). In normal hepatocytes, PRDX2 maintains redox balance and metabolic homeostasis under oxidative stress. In contrast, during malignant transformation, PRDX2 promotes oncogenic signaling, stemness, and tumor initiation ...
Naroa Goikoetxea‐Usandizaga, María Luz Martinez‐Chantar, Carolina Conter   +2 more
wiley   +1 more source

Democratising high performance computing for bioinformatics through serverless cloud computing: A case study on CRISPR-Cas9 guide RNA design with Crackling Cloud. [PDF]

PLoS Comput Biol
Bradford J, Joy D, Winsen M, Meurant N, Wilkins M, Wilson LOW, Bauer DC, Perrin D.   +7 more
europepmc   +1 more source

Structure-guided chemical modification of guide RNA enables potent non-viral in vivo genome editing

Nature Biotechnology, 2017
Hao Yin, Chun‐Qing Song, Sneha Suresh, Qiongqiong Wu, Stephen Walsh, Luke H. Rhym, Esther Mintzer, M. Bolukbasi, L. Zhu, Kevin J. Kauffman, Haiwei Mou, Alicia Oberholzer, Junmei Ding, Suet‐Yan Kwan, R. Bogorad, T. Zatsepin, V. Koteliansky, S. Wolfe, Wen Xue, R. Langer, Daniel G. Anderson   +20 more
semanticscholar   +1 more source

Dammarenediol II enhances etoposide‐induced apoptosis by targeting O‐GlcNAc transferase and Akt/GSK3β/mTOR signaling in liver cancer

Molecular Oncology, EarlyView.
Etoposide induces DNA damage, activating p53‐dependent apoptosis via caspase‐3/7, which cleaves PARP1. Dammarenediol II enhances this apoptotic pathway by suppressing O‐GlcNAc transferase activity, further decreasing O‐GlcNAcylation. The reduction in O‐GlcNAc levels boosts p53‐driven apoptosis and influences the Akt/GSK3β/mTOR signaling pathway ...
Jaehoon Lee, Byung‐Cheol Han, Gi‐Bang Koo, Jihye Park, Mijin Kwon, Young Bin Park, Jae‐Mun Choi, Seung‐Ho Lee, Sangho Roh   +8 more
wiley   +1 more source

Genome-wide CRISPR guide RNA design and specificity analysis with GuideScan2. [PDF]

Genome Biol
Schmidt H, Zhang M, Chakarov D, Bansal V, Mourelatos H, Sánchez-Rivera FJ, Lowe SW, Ventura A, Leslie CS, Pritykin Y.   +9 more
europepmc   +1 more source

Utilizing directed evolution to interrogate and optimize CRISPR/Cas guide RNA scaffolds. [PDF]

Cell Chem Biol, 2023
Bush K, Corsi GI, Yan AC, Haynes K, Layzer JM, Zhou JH, Llanga T, Gorodkin J, Sullenger BA.   +8 more
europepmc   +1 more source

Genome-scale deletion screening of human long non-coding RNAs using a paired-guide RNA CRISPR–Cas9 library

Nature Biotechnology, 2016
Shiyou Zhu, Wei Li, Jingze Liu, Chenhao Chen, Qiugang Liao, Ping Xu, Han Xu, T. Xiao, Zhongzheng Cao, Jingyu Peng, Pengfei Yuan, Myles A. Brown, Xiaole Shirley Liu, Wensheng Wei   +13 more
semanticscholar   +1 more source