Results 41 to 50 of about 570,529 (349)
Mitochondrial RNA editing in Trypanoplasma borreli: New tools, new revelations
Computational and Structural Biotechnology Journal, 2022 The kinetoplastids are unicellular flagellates that derive their name from the ‘kinetoplast’, a region within their single mitochondrion harboring its organellar genome of high DNA content, called kinetoplast (k) DNA.
Evgeny S. Gerasimov +8 more
doaj
Traffic into silence: endomembranes and post-transcriptional RNA silencing. [PDF]
, 2014 microRNAs (miRNAs) and small interfering RNAs (siRNAs) are small RNAs that repress gene expression at the post-transcriptional level in plants and animals. Small RNAs guide Argonaute-containing RNA-induced silencing complexes to target RNAs in a sequence-
Chen, Xuemei +2 more
core +3 more sources
DNA editing in DNA/RNA hybrids by adenosine deaminases that act on RNA. [PDF]
, 2017 Adenosine deaminases that act on RNA (ADARs) carry out adenosine (A) to inosine (I) editing reactions with a known requirement for duplex RNA. Here, we show that ADARs also react with DNA/RNA hybrid duplexes.
Beal, Peter A +2 more
core +1 more source
Optimized Plasmid Construction Strategy for Cas9
Cellular Physiology and Biochemistry, 2018 Background/Aims: The target genome editing technology not only plays an important role in basic biology studies but also holds a great promise for potential clinical applications.
Jianyong Xu +5 more
doaj +1 more source
Trim25 is an RNA-specific activator of Lin28a/TuT4-mediated uridylation [PDF]
, 2014 SummaryRNA binding proteins have thousands of cellular RNA targets and often exhibit opposite or passive molecular functions. Lin28a is a conserved RNA binding protein involved in pluripotency and tumorigenesis that was previously shown to trigger TuT4 ...
Ascano +45 more
core +3 more sources
Nature Methods, 2013 Targeted gene modification can be guided by programmable RNA in bacteria, zebrafish and mammalian cells.
openaire +2 more sources
RNA-Guided RNA Cleavage by a CRISPR RNA-Cas Protein Complex [PDF]
Cell, 2009 Compelling evidence indicates that the CRISPR-Cas system protects prokaryotes from viruses and other potential genome invaders. This adaptive prokaryotic immune system arises from the clustered regularly interspaced short palindromic repeats (CRISPRs) found in prokaryotic genomes, which harbor short invader-derived sequences, and the CRISPR-associated (
Michael O. Duff +7 more
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Dynamic interactions within sub-complexes of the H/ACA pseudouridylation guide RNP [PDF]
, 2007 H/ACA RNP complexes change uridines to pseudouridines in target non-coding RNAs in eukaryotes and archaea. H/ACA RNPs are comprised of a guide RNA and four essential proteins: Cbf5 (pseudouridine synthase), L7Ae, Gar1 and Nop10 in archaea.
Terns, Michael P. +2 more
core +4 more sources
A guide to ions and RNA structure [PDF]
RNA, 2004 RNA folding into stable tertiary structures is remarkably sensitive to the concentrations and types of cations present; an understanding of the physical basis of ion-RNA interactions is therefore a prerequisite for a quantitative accounting of RNA stability.
openaire +3 more sources
The Molecular Architecture for RNA-Guided RNA Cleavage by Cas13a [PDF]
Cell, 2017 Cas13a, a type VI-A CRISPR-Cas RNA-guided RNA ribonuclease, degrades invasive RNAs targeted by CRISPR RNA (crRNA) and has potential applications in RNA technology. To understand how Cas13a is activated to cleave RNA, we have determined the crystal structure of Leptotrichia buccalis (Lbu) Cas13a bound to crRNA and its target RNA, as well as the cryo-EM ...
Jiuyu Wang +8 more
openaire +3 more sources