Results 41 to 50 of about 4,450,920 (398)
Chemical modifications of adenine base editor mRNA and guide RNA expand its application scope
Nature Communications, 2020 CRISPR-Cas9-associated base editing is a promising tool to correct pathogenic single nucleotide mutations in research or therapeutic settings. Efficient base editing requires cellular exposure to levels of base editors that can be difficult to attain in ...
Tingting Jiang +16 more
semanticscholar +1 more source
Optimized CRISPR guide RNA design for two high-fidelity Cas9 variants by deep learning
Nature Communications, 2019 Highly specific Cas9 nucleases derived from SpCas9 are valuable tools for genome editing, but their wide applications are hampered by a lack of knowledge governing guide RNA (gRNA) activity. Here, we perform a genome-scale screen to measure gRNA activity
semanticscholar +1 more source
The 5' → 3' exoribonuclease XRN1/Pacman and its functions in cellular processes and development [PDF]
, 2012 XRN1 is a 5' → 3' processive exoribonuclease that degrades mRNAs after they have been decapped. It is highly conserved in all eukaryotes, including homologs in Drosophila melanogaster (Pacman), Caenorhabditis elegans (XRN1), and Saccharomyces cerevisiae (
Jones, Christopher Iain +2 more
core +1 more source
Nature Methods, 2013 Targeted gene modification can be guided by programmable RNA in bacteria, zebrafish and mammalian cells.
openaire +2 more sources
Encoding folding paths of RNA switches [PDF]
, 2006 RNA co-transcriptional folding has long been suspected to play an active role in helping proper native folding of ribozymes and structured regulatory motifs in mRNA untranslated regions.
Harlepp, S. +3 more
core +3 more sources
Guide-substrate base-pairing requirement for box H/ACA RNA-guided RNA pseudouridylation [PDF]
RNA, 2018 Box H/ACA RNAs are a group of small RNAs found in abundance in eukaryotes (as well as in archaea). Although their sequences differ, eukaryotic box H/ACA RNAs all share the same unique hairpin-hinge-hairpin-tail structure. Almost all of them function as guides that primarily direct pseudouridylation of rRNAs and spliceosomal snRNAs at specific sites ...
De Zoysa, Meemanage D. +3 more
openaire +2 more sources
Inhibition of HIV-1 replication using the CRISPR/cas9-no NLS system as a prophylactic strategy
Heliyon, 2022 Globally, it is estimated that 43 million people are living with human immunodeficiency virus type 1 (HIV-1), and there are more than 600,000 acquired immunodeficiency syndrome (AIDS)-related deaths in 2020.
Ali Salimi-Jeda +5 more
doaj +1 more source
High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity [PDF]
, 2013 The RNA-programmable Cas9 endonuclease cleaves double-stranded DNA at sites complementary to a 20-base-pair guide RNA. The Cas9 system has been used to modify genomes in multiple cells and organisms, demonstrating its potential as a facile genome ...
D Hockemeyer +26 more
core +1 more source
RNA-programmed genome editing in human cells
eLife, 2013 Type II CRISPR immune systems in bacteria use a dual RNA-guided DNA endonuclease, Cas9, to cleave foreign DNA at specific sites. We show here that Cas9 assembles with hybrid guide RNAs in human cells and can induce the formation of double-strand DNA ...
Martin Jinek +5 more
doaj +1 more source
Nature Communications, 2020 Three-dimensional (3D) structures of the genome are dynamic, heterogeneous and functionally important. Live cell imaging has become the leading method for chromatin dynamics tracking.
P. A. Clow +5 more
semanticscholar +1 more source