Rhizobium rhizogenes-mediated hairy root induction and plant regeneration for bioengineering citrus. [PDF]
Plant Biotechnol J, 2023 Plant transformation is widely used in functional genetics and bioengineering (BE)-based crop improvement. With the advent and deployment of CRISPR-based genome editing technology and increased public awareness of the safety of BE crops, the applications
Ramasamy M +5 more
europepmc +4 more sources
Hairy CRISPR: Genome Editing in Plants Using Hairy Root Transformation [PDF]
Plants, 2021 CRISPR/Cas-mediated genome editing is a powerful tool of plant functional genomics. Hairy root transformation is a rapid and convenient approach for obtaining transgenic roots. When combined, these techniques represent a fast and effective means of studying gene function.
Alexey S. Kiryushkin +4 more
openaire +4 more sources
Plant Methods, 2021 Background Agrobacterium-mediated genetic transformation is a widely used and efficient technique for gene functional research in crop breeding and plant biology.
Yuanyuan Cheng +5 more
doaj +2 more sources
Plant Methods, 2021 Background Litchi chinensis Sonn. is an economically important fruit tree in tropical and subtropical regions. However, litchi functional genomics is severely hindered due to its recalcitrance to regeneration and stable transformation.
Yaqi Qin +6 more
doaj +2 more sources
Isolation and Biophysical Characterization of Extracellular Vesicles from Hairy Root Cultures. [PDF]
Bio ProtocConte M +6 more
europepmc +2 more sources
Elicitation: A biotechnological tool for enhanced production of secondary metabolites in hairy root cultures [PDF]
Engineering in Life Sciences, 2019 Elicitation is a possible aid to overcome various difficulties associated with the large‐scale production of most commercially important bioactive secondary metabolites from wild and cultivated plants, undifferentiated or differentiated cultures ...
Mihir Halder +2 more
openalex +2 more sources
First Report of Agrobacterium rhizogenes-induced Hairy Root Formation in Selaginella bryopteris: a Pteridophyte Recalcitrant to Genetic Transformation [PDF]
Brazilian Archives of Biology and Technology, 2020 we report A. rhizogenes-induced hairy root formation in S. bryopteris, a medicinally and commercially important plant. A. rhizogenes strain LBA1334 co-cultivated with explants (root, rhizophore, stem portion near the root, and stem with intact fronds ...
Ravi Shankar Singh +5 more
doaj +1 more source
Enhancing Centelloside Production in Centella asiatica Hairy Root Lines through Metabolic Engineering of Triterpene Biosynthetic Pathway Early Genes. [PDF]
Plants (Basel), 2023 Alcalde MA +3 more
europepmc +3 more sources
Assessment of hairy roots induction in Echium khuzistanicum by different strains of Agrobacterium rhizogenes [PDF]
مجله بیوتکنولوژی کشاورزی, 2020 Objective In this study for the first time, different strains of Agrobacterium rhizogenes, co-cultivation times, and concentrations of acetosyringon in leaf explants of Echium khuzistanicum were examined to produce hairy roots.
samira mohammadi +2 more
doaj +1 more source
The Untapped Potential of Hairy Root Cultures and Their Multiple Applications. [PDF]
Int J Mol SciPlants are rich sources of specialized metabolites, such as alkaloids, terpenes, phenolic acids, flavonoids, coumarins, and volatile oils, which provide various health benefits including anticancer, anti-inflammatory, antiaging, skin-altering, and anti ...
Mirmazloum I, Slavov AK, Marchev AS.
europepmc +2 more sources