Results 21 to 30 of about 16,684 (344)

Optimization of in vitro and ex vitro Agrobacterium rhizogenes-mediated hairy root transformation of soybean for visual screening of transformants using RUBY

open access: yesFrontiers in Plant Science, 2023
In vitro and ex vitro Agrobacterium rhizogenes-mediated hairy root transformation (HRT) assays are key components of the plant biotechnology and functional genomics toolkit.
M. Niazian   +4 more
semanticscholar   +1 more source

CRISPR/Cas9 in Planta Hairy Root Transformation: A Powerful Platform for Functional Analysis of Root Traits in Soybean

open access: yesPlants, 2022
Sequence and expression data obtained by next-generation sequencing (NGS)-based forward genetics methods often allow the identification of candidate causal genes. To provide true experimental evidence of a gene’s function, reverse genetics techniques are
M. Niazian, F. Belzile, D. Torkamaneh
semanticscholar   +1 more source

An Efficient Agrobacterium rhizogenes-Mediated Hairy Root Transformation Method in a Soybean Root Biology Study

open access: yesInternational Journal of Molecular Sciences, 2022
The stable genetic transformation of soybean is time-consuming and inefficient. As a simple and practical alternative method, hairy root transformation mediated by Agrobacterium rhizogenes is widely applied in studying root-specific processes, nodulation,
Penghui Huang   +7 more
semanticscholar   +1 more source

An Efficient Hairy Root System for Validation of Plant Transformation Vector and CRISPR/Cas Construct Activities in Cucumber (Cucumis sativus L.)

open access: yesFrontiers in Plant Science, 2022
Hairy root induction system has been applied in various plant species as an effective method to study gene expression and function due to its fast-growing and high genetic stability.
Doai Van Nguyen   +7 more
semanticscholar   +1 more source

Targeted metabolomics unveil alteration in accumulation and root exudation of flavonoids as a response to interspecific competition

open access: yesJournal of Plant Interactions, 2021
This study aimed to elucidate the chemical response of hairy vetch (Vicia villosa Roth) to interspecific competition by rye (Secale cereale L.). Hairy vetch plants were grown as target plants in a mixture with different densities of rye plants (0, 1, 2 ...
Hossein Hazrati   +2 more
doaj   +1 more source

Effect of iron oxide nanoparticles on hairy root induction and antioxidant activity in Purslane (Portulaca oleracea) [PDF]

open access: yesمجله بیوتکنولوژی کشاورزی, 2021
Objective Many secondary metabolites produced by plants are used in manufacturing drugs and new treatments, but are too difficult, expensive or impossible to prepared by chemical synthesis.
Mehdi Mohebodini, Roghayeh Fathi
doaj   +1 more source

Optimization of hairy root culture establishment in Chicory plants (Cichorium intybus) through inoculation by Agrobacterium rhizogenes [PDF]

open access: yesمجله بیوتکنولوژی کشاورزی, 2013
Agrobacterium rhizogenes induced hairy roots gain of the potential of production and accumulation of plant secondary metabolites. In recent decades, many researchers have focused on the biosynthesis of valuable secondary metabolites in hairy roots ...
Sara kabirnotaj   +3 more
doaj   +1 more source

An efficient genetic transformation system mediated by Rhizobium rhizogenes in fruit trees based on the transgenic hairy root to shoot conversion. [PDF]

open access: yesPlant Biotechnol J
Summary Genetic transformation is a critical tool for gene editing and genetic improvement of plants. Although many model plants and crops can be genetically manipulated, genetic transformation systems for fruit trees are either lacking or perform poorly.
Liu L   +13 more
europepmc   +2 more sources

Effect of different Agrobacterium rhizogenes strains for in-vitro hairy root induction, total phenolic, flavonoids contents, antibacterial and antioxidant activity of (Cucumis anguria L.)

open access: hybridSaudi Journal of Biological Sciences, 2020
The present study aimed to investigate the effect of different Agrobacterium rhizogenes on the induction of hairy root of Cucumis anguria and determine its total phenolic, flavonoids contents, antibacterial and antioxidant activity. In this investigation
Jesudass Joseph Sahayarayan   +6 more
openalex   +2 more sources

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