Results 21 to 30 of about 1,132,099 (329)

CRISPR/Cas9 in Planta Hairy Root Transformation: A Powerful Platform for Functional Analysis of Root Traits in Soybean

open access: yesPlants, 2022
Sequence and expression data obtained by next-generation sequencing (NGS)-based forward genetics methods often allow the identification of candidate causal genes. To provide true experimental evidence of a gene’s function, reverse genetics techniques are
M. Niazian, F. Belzile, D. Torkamaneh
semanticscholar   +1 more source

The Influence of Exogenous Jasmonic Acid on the Biosynthesis of Steroids and Triterpenoids in Calendula officinalis Plants and Hairy Root Culture

open access: yesInternational Journal of Molecular Sciences, 2022
The interplay between steroids and triterpenoids, compounds sharing the same biosynthetic pathway but exerting distinctive functions, is an important part of the defense strategy of plants, and includes metabolic modifications triggered by stress ...
Agata Rogowska   +3 more
semanticscholar   +1 more source

Hairy Root Induction in Helicteres isora L. and Production of Diosgenin in Hairy Roots [PDF]

open access: yesNatural Products and Bioprospecting, 2014
Mature seeds of Helicteres isora L. were collected from seven geographical locations of Maharashtra and Goa (India) and evaluated for diosgenin (a bioactive steroidal sapogenin of prime importance) extraction and quantification. Chemotypic variations were evidenced with diosgenin quantity ranging from 33 μg g(-1) seeds (Osmanabad forests) to 138 μg g ...
Vinay Kumar   +2 more
openaire   +2 more sources

Targeted metabolomics unveil alteration in accumulation and root exudation of flavonoids as a response to interspecific competition

open access: yesJournal of Plant Interactions, 2021
This study aimed to elucidate the chemical response of hairy vetch (Vicia villosa Roth) to interspecific competition by rye (Secale cereale L.). Hairy vetch plants were grown as target plants in a mixture with different densities of rye plants (0, 1, 2 ...
Hossein Hazrati   +2 more
doaj   +1 more source

Effect of iron oxide nanoparticles on hairy root induction and antioxidant activity in Purslane (Portulaca oleracea) [PDF]

open access: yesمجله بیوتکنولوژی کشاورزی, 2021
Objective Many secondary metabolites produced by plants are used in manufacturing drugs and new treatments, but are too difficult, expensive or impossible to prepared by chemical synthesis.
Mehdi Mohebodini, Roghayeh Fathi
doaj   +1 more source

Optimization of hairy root culture establishment in Chicory plants (Cichorium intybus) through inoculation by Agrobacterium rhizogenes [PDF]

open access: yesمجله بیوتکنولوژی کشاورزی, 2013
Agrobacterium rhizogenes induced hairy roots gain of the potential of production and accumulation of plant secondary metabolites. In recent decades, many researchers have focused on the biosynthesis of valuable secondary metabolites in hairy roots ...
Sara kabirnotaj   +3 more
doaj   +1 more source

Transformation and Detection of Soybean Hairy Roots

open access: yesBIO-PROTOCOL, 2023
Agrobacterium rhizogenes is a soil bacteria with extensive infectivity, which can infect almost all dicotyledonous plants and a few monocotyledonous plants to induce root nodules. This is caused by the root-inducing plasmid, which contains genes responsible for the autonomous growth of root nodules and crown gall base synthesis.
Xing Xu   +9 more
openaire   +3 more sources

A protocol for the generation of Arachis hypogaea composite plants: A valuable tool for the functional study of mycorrhizal symbiosis

open access: yesApplications in Plant Sciences, 2022
Premise Agrobacterium rhizogenes–induced hairy root systems are one of the most preferred and versatile systems for the functional characterization of genes.
Kalpana Nanjareddy   +2 more
doaj   +1 more source

An Optimized Tobacco Hairy Root Induction System for Functional Analysis of Nicotine Biosynthesis-Related Genes

open access: yesAgronomy, 2022
Rhizobium rhizogenes-mediated plant hairy root induction is a convenient method for functional study of root-specific genes. To develop an optimized tobacco hairy root induction system and study gene function in nicotine biosynthesis, we investigated ...
Shangqian Qin   +5 more
doaj   +1 more source

Poison ivy hairy root cultures enable a stable transformation system suitable for detailed investigation of urushiol metabolism

open access: yesPlant Direct, 2020
Poison ivy (Toxicodendron radicans) is best known for causing exasperating allergenic delayed‐contact dermatitis symptoms that last for weeks on persons who have contacted the plant.
Aneirin A. Lott   +5 more
doaj   +1 more source

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