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Anthraquinones from hairy root cultures of Cassia obtusifolia
Phytochemistry, 1998Hairy root cultures of Cassia obtusifolia clones transformed with Agrobacterium rhizogenes strain 9402 were established to investigate the anthraquinone production. Seven anthraquinones, together with betulinic acid, stigmasterol and sitosterol were isolated from the hairy roots. Their chemical structures were elucidated on the basis of chromatographic
Hongzhu, Guo +4 more
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Hernandulcin in hairy root cultures of Lippia dulcis
Plant Cell Reports, 1991The hairy root culture of Lippia dulcis Trev., Verbenaceae, was established by transformation with Agrobacterium rhizogenes A4. The transformed roots grew well in Murashige and Skoog medium containing 2% sucrose. The roots turned light green when they were cultured under 16 h/day light.
M, Sauerwein, T, Yamazaki, K, Shimomura
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The multiplicity of hairy root cultures: Prolific possibilities
Plant Science, 2011Hairy root cultures (HRCs), induced by Agrobacterium rhizogenes infection, have been established from a wide variety of plant species. HRCs accumulate phytochemicals to levels comparable to that of intact plants and are usually stable in their biosynthetic capacity.
Nadia N, Ono, Li, Tian
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Biotransformation studies using hairy root cultures — A review
Biotechnology Advances, 2012Agrobacterium rhizogenes induced hairy root cultures are entering into a new juncture of functional research in generating pharmaceutical lead compounds by bringing about chemical transformations aided through its inherent enzyme resources. Rational utilization of hairy root cultures as highly effective biotransformation systems has come into existence
Suchitra, Banerjee +2 more
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Hairy Root Culture: Bioreactor Design and Process Intensification
2013The cultivation of hairy roots for the production of secondary metabolites offers numerous advantages; hairy roots have a fast growth rate, are genetically stable, and are relatively simple to maintain in phytohormone free media. Hairy roots provide a continuous source of secondary metabolites, and are useful for the production of chemicals for ...
Amanda R, Stiles, Chun-Zhao, Liu
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Biotransformation of digitoxigenin by ginseng hairy root cultures
Phytochemistry, 1990Five new compounds (three esters and two glycosides) and seven previously reported compounds were isolated as biotransformation products of digitoxigenin by ginseng hairy root cultures. The new esters and glycosides were elucidated as digitoxigenin stearate, digitoxigenin palmitate, digitoxigenin myristate, 3-epidigitoxigenin beta-D-gentiobioside and ...
K, Kawaguchi +3 more
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Morphological Structured Model for Hairy Root Cultures
IFAC Proceedings Volumes, 1997The possibility of using hairy root (HR) cultures for producing secondary metabolites on a large scale has recently received a great deal of attention (Flores et al., 1987; Hamill et al., 1987; Rhodes et al, 1987; Scheidegger, 1990; Toivonen, 1993).
I. Berlin, D. Mills, J.C. Merchuk
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2012
The invention of hairy root induction from various higher plants has provided a new set of hopes for using such organs in vitro as an alternative to cell cultures, chiefly because of their genetic stabilities that impart them high biochemical consistencies.
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The invention of hairy root induction from various higher plants has provided a new set of hopes for using such organs in vitro as an alternative to cell cultures, chiefly because of their genetic stabilities that impart them high biochemical consistencies.
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Oxygen requirements and mass transfer in hairy‐root culture
Biotechnology and Bioengineering, 1994AbstractOxygen mass transfer in clumps of Atropa belladonna hairy roots was investigated as a function of root density and external flow conditions. Convection was the dominant mechanism for mass transfer into root clumps 3.5 to 5.0 cm in diameter; Peclet numbers inside the clumps ranged from 1.4 × 103 to 7.1 × 104 for external superficial flow ...
S, Yu, P M, Doran
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Alkaloid production by hairy root cultures in Atropa belladonna
Plant Cell Reports, 1986Hairy roots were induced by inoculation of stems of sterile plants of Atropa belladonna with Agrobacterium rhizogenes. The axenic culture of the hairy roots isolated from the stems proliferated 60 fold as based on the initial fresh weight after one month of culture.
H, Kamada +4 more
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