Results 261 to 270 of about 535,063 (297)

Production of monoclonal antibodies by tobacco hairy roots

Biotechnology and Bioengineering, 1997
Hairy roots of tobacco (Nicotiana tabacum) were used to produce full-length murine lgG(1) monoclonal antibody. The presence of heavy (gamma) and light (kappa) chains and fully assembled antibody was verified by Western blot analysis of root extracts. Antibody levels in the biomass and medium were quantified by ELISA based on detection of gamma-kappa ...
Pauline M Doran
exaly   +3 more sources

Hairy roots induced by Agrobacterium rhizogenes and production of regenerative plants in hairy root cultures in maize

Science in China Series C: Life Sciences, 2006
Hairy roots of maize were induced by infecting 15-d calli with Agrobacterium rhizogenes. The hairy roots cultured in hormone-free media showed the vigorous growth and typical hairy root features. The regenerated plants were produced from hairy roots in MS media supplemented with 1.6 mg/L ZT and 0.4 mg/L NAA.
Hongwei, Xu, Xiaofu, Zhou, Jingmei, Lu, Junjie, Wang, Xingzhi, Wang   +4 more
openaire   +2 more sources

Hairy Root-activation Tagging: a High-throughput System for Activation Tagging in Transformed Hairy Roots

Plant Molecular Biology, 2005
Activation tagging is a powerful technique for generating gain-of-function mutants in plants. We developed a new vector system for activation tagging of genes in "transformed hairy roots". The binary vector pHR-AT (Hairy Root-Activation Tagging) and its derivative pHR-AT-GFP contain a cluster of rol (rooting locus) genes together with the right border ...
Hikaru, Seki, Tomoko, Nishizawa, Nobukazu, Tanaka, Yasuo, Niwa, Shigeo, Yoshida, Toshiya, Muranaka   +5 more
openaire   +2 more sources

Establishment of hairy root cultures of Psoralea species

Plant Cell Reports, 1992
Eight Psoralea species (Leguminosae) were inoculated with Agrobacterium rhizogenes, strains 8196 and 9402. Hairy roots were only induced by strain 9402. Attention was focussed on Psoralea lachnostachys. Transformed roots grew very rapidly in Gamborg B5 liquid medium with a doubling time of the culture of 38 hours.
Nguyen, Christophe, Bourgaud, Frederic, Forlot, Paul, Guckert, Armand   +3 more
openaire   +3 more sources

Biotransformation of digitoxigenin by ginseng hairy root cultures

Phytochemistry, 1990
Five new compounds (three esters and two glycosides) and seven previously reported compounds were isolated as biotransformation products of digitoxigenin by ginseng hairy root cultures. The new esters and glycosides were elucidated as digitoxigenin stearate, digitoxigenin palmitate, digitoxigenin myristate, 3-epidigitoxigenin beta-D-gentiobioside and ...
K, Kawaguchi, M, Hirotani, T, Yoshikawa, T, Furuya   +3 more
openaire   +2 more sources

Large-Scale Production of Hairy Root

2004
Many products of interest are synthesized in organized tissues, but not formed in suspension or callus culture. Therefore, most attention has been focused on root cultures. The transgenic plant,"hairy root", has brought us to dramatic improvements in growth rate and high content of desirable products.
openaire   +2 more sources

Establishment of hairy root cultures of Ammi majus

Plant Science, 2001
Axenically grown Ammi majus plantlets were inoculated with seven different Agrobacterium rhizogenes strains. Hairy root lines were established only after inoculation with the two agropine strains: A4 and LBA9402. The growth rate of hairy root cultures was about thirty times faster than that of callus and cell suspension cultures.
Aleksandra Królicka, Izabela Staniszewska, Krzysztof Piotr Bielawski, Edmund Maliński, Janusz Szafranek, Ewa Łojkowska   +5 more
openaire   +2 more sources

Polyacetylenes in Hairy Roots of aPanaxHybrid

Planta Medica, 2003
From the hairy roots of an interspecific hybrid ginseng ( Panax ginseng x P. quinquefolium) known as Pgq, three polyacetylenes were isolated: panaxynol, panaxydol and 1,8-heptadecadiene-3,10-diol. These compounds isolated from the hairy roots were used for quantitative analysis to investigate the polyacetylene production of the hairy roots cultured in ...
Daisuke, Washida, Koichiro, Shimomura, Susumu, Kitanaka   +2 more
openaire   +2 more sources

Cryopreservation of Transformed (Hairy) Roots ofArtemisia annua

Cryobiology, 1996
The antimalarial drug artemisinin has been found in transformed (hairy) roots of Artemisia annua. A protocol was developed to preserve A. annua hairy roots in liquid nitrogen. Root tips were excised from 7-day-old cultures and held on solid White's medium for 24 h prior to cryoprotection.
K H, Teoh, P J, Weathers, R D, Cheetham, D B, Walcerz   +3 more
openaire   +2 more sources

Hairy Root Cultures for Secondary Metabolites Production

2010
Hairy roots (HRs) are differentiated cultures of transformed roots generated by the infection of wounded higher plants with Agrobacterium rhizogenes. This pathogen causes the HR disease leading to the neoplastic growth of roots that are characterized by high growth rate in hormone free media and genetic stability.
PISTELLI, LAURA, Giovannini, A., Ruffoni, B., BERTOLI, ALESSANDRA, PISTELLI, LUISA   +4 more
openaire   +2 more sources