Results 101 to 110 of about 4,066 (207)
A Small RNA Is Linking CRISPR–Cas and Zinc Transport
The function and mode of action of small regulatory RNAs is currently still understudied in archaea. In the halophilic archaeon Haloferax volcanii, a plethora of sRNAs have been identified; however, in-depth functional analysis is missing for most of ...
Pascal Märkle +7 more
doaj +1 more source
Two Decades of Optogenetic Tools: A Retrospective and a Look Ahead
Optogenetics provides up to millisecond scale, cell type‐specific control of biology using light‐sensitive proteins. This review chronicles two decades of tool development, from microbial rhodopsins and photoactivated enzymes to allosteric switches, surveys broad applications in neuroscience, molecular and cell biology, cardiology, immunology, and ...
Xiao Duan, Mo Zhu, Shiqiang Gao
wiley +1 more source
Genotypic and Lipid Analyses of Strains From the Archaeal Genus Halorubrum Reveal Insights Into Their Taxonomy, Divergence, and Population Structure [PDF]
To gain a better understanding of how divergence occurs, and how taxonomy can benefit from studying natural populations, we isolated and examined 25 closely related Halorubrum strains obtained from different hypersaline communities and compared them to ...
Corral Villa, Paulina +4 more
core +1 more source
Background Haloarchaea, a major group of archaea, are able to metabolize sugars and to live in oxygenated salty environments. Their physiology and lifestyle strongly contrast with that of their archaeal ancestors.
Raphaël Méheust +5 more
doaj +1 more source
Small RNA Toxin‐Assisted Evolution of GC‐Preferred ErCas12a for Enhanced Genome Targeting Range
ErCas12a is engineered to target GC‐rich PAMs using a small RNA toxin‐aided positive screening system. The resulting variant, enErCas12a, exhibits an expanded PAM profile and facilitates efficient gene editing in both bacterial and mammalian cells, while preserving high targeting specificity for both canonical and non‐canonical PAM targets.
Zehua Chen +8 more
wiley +1 more source
Impacts of oil palm expansion on avian biodiversity in a Neotropical natural savanna [PDF]
The consequences of converting tropical rainforest to oil palm are well-documented, but the impacts of oil palm conversion on natural savanna landscapes remain little-known.
Edwards, David P. +3 more
core +1 more source
Improved GFP Variants to Study Gene Expression in Haloarchaea
The study of promoter activities in haloarchaea is carried out exclusively using enzymes as reporters. An alternative reporter is the gene encoding the Green Fluorescent Protein (GFP), a simple and fast tool for investigating promoter strengths. However,
Johannes Born, Felicitas Pfeifer
doaj +1 more source
Diversity of DNA replication in the archaea [PDF]
DNA replication is arguably the most fundamental biological process. On account of their shared evolutionary ancestry, the replication machinery found in archaea is similar to that found in eukaryotes.
Akita +6 more
core +3 more sources
A haloarchaeal ferredoxin electron donor that plays an essential role in nitrate assimilation [PDF]
In the absence of ammonium, many organisms, including the halophilic archaeon Haloferax volcanii DS2 (DM3757), may assimilate inorganic nitrogen from nitrate or nitrite, using a ferredoxin-dependent assimilatory NO3-/NO2- reductase pathway.
Bonete, Maria José +5 more
core +1 more source
Following the previous complete genome analysis, this research provides the function of new Halobacillus species. Three strains were identified: Halobacillus shinanisalinarum sp. nov. SSTM10‐2T, Halobacillus salinarum sp. nov. SSBR10‐3T, and Halobacillus amylolyticus sp. nov. SSHM10‐5T.
Saru Gurung +4 more
wiley +1 more source

