Inhibiting NAD+-dependent DNA ligase activity with 2-(cyclopentyloxy)-5'-deoxyadenosine (CPOdA) offers a new tool for DNA replication and repair studies in the model archaeon Haloferax volcanii [PDF]
, 2015 DNA ligases play an essential role in many aspects of DNA metabolism in all three domains of life. The haloarchaeal organism Haloferax volcanii encodes both ATP- and NAD+-dependent DNA ligase enzymes designated LigA and LigN, respectively.
Giroux, Xavier, MacNeill, Stuart
core +4 more sources
MinD2 modulates cell shape and motility in the archaeon Haloferax volcanii [PDF]
Frontiers in MicrobiologyIn bacteria and archaea, proteins of the ParA/MinD family of ATPases regulate the spatiotemporal organization of various cellular cargoes, including cell division proteins, motility structures, chemotaxis systems, and chromosomes.
Megha Patro +11 more
doaj +2 more sources
The Adaptive Immune System of Haloferax volcanii [PDF]
Life, 2015 To fight off invading genetic elements, prokaryotes have developed an elaborate defence system that is both adaptable and heritable—the CRISPR-Cas system (CRISPR is short for: clustered regularly interspaced short palindromic repeats and Cas: CRISPR ...
Lisa-Katharina Maier +2 more
doaj +3 more sources
Chase Assay of Protein Stability in Haloferax volcanii
Bio-Protocol, 2017 Highly regulated and targeted protein degradation plays a fundamental role in almost all cellular processes. Determination of the protein half-life by the chase assay serves as a powerful and popular strategy to compare the protein stability and study ...
Xian Fu, Julie Maupin-Furlow
doaj +4 more sources
N-glycosylation in Haloferax volcanii: Adjusting the sweetness [PDF]
Frontiers in Microbiology, 2013 Long believed to be restricted to Eukarya, it is now known that cells of all three domains of life perform N-glycosylation, the covalent attachment of glycans to select target protein asparagine residues.
Jerry eEichler +5 more
doaj +3 more sources
Growth phenotype analysis of heme synthetic enzymes in a halophilic archaeon, Haloferax volcanii. [PDF]
PLoS ONE, 2017 Halophilic euryarchaea lack many of the genes necessary for the protoporphyrin-dependent heme biosynthesis pathway previously identified in animals and plants.
Naoki Kosugi +4 more
doaj +3 more sources
The complete genome sequence of Haloferax volcanii DS2, a model archaeon.
PLoS ONE, 2010 BackgroundHaloferax volcanii is an easily culturable moderate halophile that grows on simple defined media, is readily transformable, and has a relatively stable genome.
Amber L Hartman +15 more
doaj +2 more sources
Haloferax volcanii—a model archaeon for studying DNA replication and repair [PDF]
Open Biology, 2020 The tree of life shows the relationship between all organisms based on their common ancestry. Until 1977, it comprised two major branches: prokaryotes and eukaryotes. Work by Carl Woese and other microbiologists led to the recategorization of prokaryotes
Patricia Pérez-Arnaiz +3 more
doaj +2 more sources
DPANN symbiont of Haloferax volcanii accelerates xylan degradation by the non-host haloarchaeon Halorhabdus sp. [PDF]
iScienceSummary: This study examines a natural consortium of halophilic archaea, comprising xylan-degrading Halorhabdus sp. SVX81, consortium cohabitant Haloferax volcanii SVX82 (formerly H. lucentense SVX82), and its DPANN ectosymbiont Ca.
Oleg N. Reva +12 more
doaj +2 more sources
Revisiting synthetic lethality of Gcn5-related N-acetyltransferase (GNAT) family mutations in Haloferax volcanii [PDF]
Microbiology SpectrumLysine acetylation is a post-translational modification that occurs in all domains of life, highlighting its evolutionary significance. Previous genome comparison identified three Gcn5-related N-acetyltransferase (GNAT) family members as lysine ...
Katherine R. Weber +2 more
doaj +2 more sources