An expression vector for the archaebacterium Haloferax volcanii [PDF]
The recent development of an efficient transformation method and shuttle vectors for Haloferax volcanii has set the stage for rapid progress in archaebacterial molecular biology. We describe a shuttle-expression vector that can be selected for and maintained in either H.
D T, Nieuwlandt, C J, Daniels
openaire +2 more sources
The ability to form biofilms is shared by many microorganisms, including archaea. Cells in a biofilm are encased in extracellular polymeric substances that typically include polysaccharides, proteins, and extracellular DNA, conferring protection while ...
Heather Schiller +8 more
doaj +1 more source
DNA Binding in High Salt: Analysing the Salt Dependence of Replication Protein A3 from the Halophile Haloferax volcanii [PDF]
Halophilic archaea maintain intracellular salt concentrations close to saturation to survive in high-salt environments and their cellular processes have adapted to function under these conditions.
Bunting, KA, Patoli, B, Winter, JA
core +2 more sources
A haloarchaeal ferredoxin electron donor that plays an essential role in nitrate assimilation [PDF]
In the absence of ammonium, many organisms, including the halophilic archaeon Haloferax volcanii DS2 (DM3757), may assimilate inorganic nitrogen from nitrate or nitrite, using a ferredoxin-dependent assimilatory NO3-/NO2- reductase pathway.
Bonete, Maria José +5 more
core +1 more source
Archaeal S-layer glycoproteins: post-translational modification in the face of extremes [PDF]
Corresponding to the sole or basic component of the surface (S)-layer surrounding the archaeal cell in most known cases, S-layer glycoproteins are in direct contact with the harsh environments that characterize niches where Archaea can thrive ...
Jerry Eichler, Lina Kandiba
core +2 more sources
Translational coupling via termination-reinitiation in archaea and bacteria
Archaea and bacteria often have gene pairs with overlapping stop and start codons, suggesting translational coupling. Here, Huber et al. analyse overlapping gene pairs from 720 genomes, and validate translational coupling via termination-reinitiation for
Madeleine Huber +8 more
doaj +1 more source
Co-expression and purification of the RadA recombinase with the RadB paralog from Haloferax volcanii yields heteromeric ring-like structures [PDF]
The study of archaeal proteins and the processes to which they contribute poses particular challenges due to the often extreme environments in which they function.
Atif A. Patoli +8 more
core +2 more sources
Diversity of DNA replication in the archaea [PDF]
DNA replication is arguably the most fundamental biological process. On account of their shared evolutionary ancestry, the replication machinery found in archaea is similar to that found in eukaryotes.
Akita +6 more
core +3 more sources
Cytochrome aa 3 in Haloferax volcanii [PDF]
ABSTRACT A cytochrome in an extremely halophilic archaeon, Haloferax volcanii , was purified to homogeneity. This protein displayed a redox difference spectrum that is characteristic of a -type cytochromes and a CN − complex spectrum that indicates the ...
Mikiei, Tanaka +4 more
openaire +2 more sources
Secondary metabolites (SMs) are essential across all life domains, yet those originating from the Archaea domain remain poorly understood. Here, the systematic genome mining and the pioneering heterologous expression of archaeal SMs have revealed the chemical landscape of archaeal lanthipeptides, showing both canonical and non‐canonical forms.
Zhi‐Man Song +13 more
wiley +2 more sources

