Results 1 to 10 of about 3,102 (186)

Optogenetic ion pumps differ with respect to the secondary pattern of K+ redistribution [PDF]

Physiological Reports, 2023
We recently reported that strong activation of the optogenetic chloride pump, halorhodopsin leads to a secondary redistribution of K+ ions into the cell, through tonically open, “leak” K+ channels.
R. Ryley Parrish, Tom Jackson‐Taylor, Juha Voipio, Andrew J. Trevelyan   +3 more
doaj   +2 more sources

Light-Driven Chloride Transport Kinetics of Halorhodopsin [PDF]

Biophysical Journal, 2018
Despite growing interest in light-driven ion pumps for use in optogenetics, current estimates of their transport rates span two orders of magnitude due to challenges in measuring slow transport processes and determining protein concentration and/or orientation in membranes in vitro.
Feroz, Hasin, Ferlez, Bryan, Lefoulon, Cecile, Ren, Tingwei, Baker, Carol S., Gajewski, John P., Lugar, Daniel J., Gaudana, Sandeep B., Butler, Peter J., Hühn, Jonas, Lamping, Matthias, Parak, Wolfgang J., Hibberd, Julian M., Kerfeld, Cheryl A., Smirnoff, Nicholas, Blatt, Michael R., Golbeck, John H., Kumar, Manish   +17 more
openaire   +5 more sources

Ion-pumping microbial rhodopsin protein classification by machine learning approach [PDF]

BMC Bioinformatics, 2023
Background Rhodopsin is a seven-transmembrane protein covalently linked with retinal chromophore that absorbs photons for energy conversion and intracellular signaling in eukaryotes, bacteria, and archaea.
Muthu Krishnan Selvaraj, Anamika Thakur, Manoj Kumar, Anil Kumar Pinnaka, Chander Raman Suri, Busi Siddhardha, Senthil Prasad Elumalai   +6 more
doaj   +2 more sources

Charge Motions During the Photocycle of pharaonis Halorhodopsin [PDF]

Biophysical Journal, 2000
Oriented gel samples were prepared from halorhodopsin-containing membranes from Natronobacterium pharaonis, and their photoelectric responses to laser flash excitation were measured at different chloride concentrations. The fast component of the current signal displayed a characteristic dependency on chloride concentration, and could be interpreted as ...
Ludmann, Krisztina, Ibron, Grazyna, Lanyi, Janos K., Váró, György   +3 more
openaire   +3 more sources

Optogenetic regulation of chloride ions in reactive astrocytes may mitigate Parkinson’s disease pathology [PDF]

Scientific Reports
Recent investigations underscore the pivotal role of reactive astrocytes in the pathogenesis of Parkinson’s disease (PD). As PD advances, reactive astrocytes lose their ability to degrade α-Synuclein (α-Syn) aggregates while concurrently increasing r ...
Eun Jung Lee, Hyung Ho Yoon, Sang Ryong Jeon   +2 more
doaj   +2 more sources

Effect of a bound anion on the structure and dynamics of halorhodopsin from Natronomonas pharaonis [PDF]

Structural Dynamics, 2019
Active ion transport across membranes is vital to maintaining the electrochemical gradients of ions in cells and is mediated by transmembrane proteins. Halorhodopsin (HR) functions as a light-driven inward pump for chloride ions. The protein contains all-
Misao Mizuno, Yumi Shimoo, Hideki Kandori, Yasuhisa Mizutani   +3 more
doaj   +2 more sources

Reconstitution of purified halorhodopsin. [PDF]

Proceedings of the National Academy of Sciences, 1984
Asolectin lipid vesicles containing halorhodopsin show light-induced acidification in the presence of proton ionophores. This effect is abolished by triphenyltin chloride, a chloride/hydroxyl antiporter, and is greatly diminished by valinomycin in the presence of potassium ions, which collapse the membrane potential.
R A, Bogomolni, M E, Taylor, W, Stoeckenius   +2 more
openaire   +4 more sources

eNpHR: a Natronomonas halorhodopsin enhanced for optogenetic applications [PDF]

Brain Cell Biology, 2008
Temporally precise inhibition of distinct cell types in the intact nervous system has been enabled by the microbial halorhodopsin NpHR, a fast light-activated electrogenic Cl(-) pump. While neurons can be optically hyperpolarized and inhibited from firing action potentials at moderate NpHR expression levels, we have encountered challenges with pushing ...
Gradinaru, Viviana, Thompson, Kimberly R., Deisseroth, Karl   +2 more
openaire   +5 more sources

Capturing macromolecular dynamics using time-resolved serial crystallography at X- ray free electron lasers and synchrotron light sources [PDF]

Structural Dynamics
In recent years, time-resolved serial crystallography has emerged as a transformative technique for unraveling the intricate dynamics of macromolecules at atomic resolution.
Sandra Mous, Guillaume Gotthard, David Ehrenberg, Saumik Sen, Tobias Weinert, Philip Johnson, Daniel James, Karol Nass, Antonia Furrer, Pikyee Ma, Steffen Bruenle, Cecilia Casadei, Isabelle Martiel, Florian Dworkowski, Dardan Gashi, Petr Skopintsev, Maximilian Wranik, Gregor Knopp, Ezequiel Panepucci, Valerie Panneels, Claudio Cirelli, Dmitry Ozerov, Gebhard Schertler, Meitian Wang, Chris Milne, Joerg Standfuss, Igor Schapiro, Joachim Heberle, Przemyslaw Nogly   +28 more
doaj   +2 more sources

Bioinspired light-driven chloride pump with helical porphyrin channels [PDF]

Nature Communications
Halorhodopsin, a light-driven chloride pump, utilizes photonic energy to drive chloride ions across biological membranes, regulating the ion balance and conveying biological information.
Chao Li, Yi Zhai, Heming Jiang, Siqi Li, Pengxiang Liu, Longcheng Gao, Lei Jiang   +6 more
doaj   +2 more sources