Results 21 to 30 of about 3,121 (204)
Frontiers in Systems Neuroscience, 2011 Technologies for silencing the electrical activity of genetically-targeted neurons in the brain are important for assessing the contribution of specific cell types and pathways towards behaviors and pathologies.
Xue eHan +10 more
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Hyperpolarization by activation of halorhodopsin results in enhanced synaptic transmission: Neuromuscular junction and CNS circuit. [PDF]
PLoS ONE, 2018 Optogenetics offers a unique method to regulate the activity of select neural circuits. However, the electrophysiological consequences of targeted optogenetic manipulation upon the entire circuit remain poorly understood.
Matthew Mattingly +3 more
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Specificity, Versatility, and Continual Development: The Power of Optogenetics for Epilepsy Research
Frontiers in Cellular Neuroscience, 2018 Optogenetics is a powerful and rapidly expanding set of techniques that use genetically encoded light sensitive proteins such as opsins. Through the selective expression of these exogenous light-sensitive proteins, researchers gain the ability to ...
Zoé Christenson Wick +1 more
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Iso-halorhodopsin: a stable, 9-cis retinal containing photoproduct of halorhodopsin
Biophysical Journal, 1987 Dark-adapted halorhodopsin is a mixture of 13-cis and all-trans retinal chromophoric species. It is known that illumination with blue light increases the all-trans content, and this is reversed partially by brief red illumination. We now find that extended red-light illumination produces a third spectroscopic form.
Zimányi, L., Lanyi, J.K.
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Purification of photochemically active halorhodopsin. [PDF]
Proceedings of the National Academy of Sciences, 1983 We have developed a procedure for the purification of halorhodopsin in a photochemically active state. Solubilization of membranes from a bacteriorhodopsin-negative Halobacterium strain with octyl glucoside was followed by chromatography on hydroxylapatite and octyl-Sepharose gels. All steps were carried out in high-ionic-strength solutions.
M E, Taylor, R A, Bogomolni, H J, Weber
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Frontiers in Behavioral Neuroscience, 2014 The development of excessive fear and/or stress responses to environmental cues such as contexts associated with a traumatic event is a hallmark of post-traumatic stress disorder (PTSD).
Dennis R. Sparta +6 more
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Primary charge separation in halorhodopsin [PDF]
FEBS Letters, 1985 INTRODUCTION Halorhodopsin (hR) is a retinal-binding protein in the cell membrane of Halobacterium halobium [l-4]. On the basis of light-induced volume change measurements on cell envelope vesicles, hR has been suggested to act as a light-driven Cl- pump [5].
Dér, A. +3 more
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Functional reconstitution of halorhodopsin. Properties of halorhodopsin-containing proteoliposomes.
Journal of Biological Chemistry, 1988 A one-step purification method for halorhodopsin was developed. Functional proteoliposomes were prepared from this preparation using cholate, which is removed by dialysis in the presence of asolectin or the polar halobacterial lipids. Light-induced outward directed transport of chloride by halorhodopsin was followed by measuring passive proton efflux ...
A, Duschl, M A, McCloskey, J K, Lanyi
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Frontiers in Physiology, 2020 Non-excitable cells (NECs) such as cardiac myofibroblasts that are electrotonically coupled to cardiomyocytes affect conduction velocity (θ) by representing a capacitive load (CL: increased membrane to be charged) and a resistive load (RL: partial ...
Stefano Andrea De Simone +5 more
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Selective optical control of synaptic transmission in the subcortical visual pathway by activation of viral vector-expressed halorhodopsin. [PDF]
PLoS ONE, 2011 The superficial layer of the superior colliculus (sSC) receives visual inputs via two different pathways: from the retina and the primary visual cortex. However, the functional significance of each input for the operation of the sSC circuit remains to be
Katsuyuki Kaneda +7 more
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