Results 51 to 60 of about 37,107 (214)
Crop Breeding and Applied Biotechnology, 2022 Abstract To meet the demands of the production chain, breeding programs need to reduce the time for selection and development of higher yielding maize genotypes. This involves application of new strategies, such as the use of the doubled haploid lines. We evaluated the use of stomatal area and density in distinguishing doubled haploids (DH) from false ...
Mariana Rodrigues Ribeiro +7 more
openaire +3 more sources
Crop Breeding and Applied Biotechnology, 2023 Identification of early haploids is necessary to increase the efficiency of the double haploid production. The objectives were to determine the effectiveness of early selection of haploid seedling and to investigate the haploid inducers and donor ...
Letícia de Freitas de Souza +6 more
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Advanced Science, EarlyView.A patient‐specific, haplotype‐resolved genome framework improves detection and interpretation of somatic variants in hepatocellular carcinoma. Using multi‐platform sequencing, the personalized assembly resolves complex regions including centromeres and MHC/HLA loci, enhances structural variant discovery, and links regulatory alterations to allele ...
Jiazheng Lin +17 more
wiley +1 more source
Advanced Science, EarlyView.An optimized Cas9‐enriched nanopore sequencing workflow, combined with STRiker, enables simultaneous analysis of disease‐associated STR loci from patient blood. The nCATS–STRiker workflow detects repeat expansions, de novo repeat motifs, interruption patterns, and methylation in a single assay, improving the genetic diagnosis of previously undiagnosed ...
Seungbok Lee +11 more
wiley +1 more source
Point Mutations in Centromeric Histone Induce Post-zygotic Incompatibility and Uniparental Inheritance. [PDF]
PLoS Genetics, 2015 The centromeric histone 3 variant (CENH3, aka CENP-A) is essential for the segregation of sister chromatids during mitosis and meiosis. To better define CENH3 functional constraints, we complemented a null allele in Arabidopsis with a variety of mutant ...
Sundaram Kuppu +6 more
doaj +1 more source
Rapid and accurate identification of in vivo-induced haploid seeds based on oil content in maize [PDF]
Scientific Reports, 2013 The needs of a growing human population require rapid and efficient development of improved cultivars by plant breeders. The doubled haploid (DH) technology enables generating completely homozygous lines in a single step and, thus, is central to modern genetics and breeding approaches.
Melchinger, Albrecht E. +4 more
openaire +2 more sources
Advanced Science, EarlyView.This work presents a large‐cell‐compatible droplet microfluidic platform that overcomes long‐standing barriers in plant cell culture by enabling high‐fidelity encapsulation, novel multilayer 3D droplet incubation for scalable long‐term culture, and support for the full developmental progression of large plant cells.
Arman Mirmiran +10 more
wiley +1 more source
Production and identification of haploid dwarf male sterile wheat plants induced by corn inducer [PDF]
Botanical Studies, 2014 AbstractBackgroundUsing the cross of wheat and maize is a very useful way to produce wheat haploid plants by chromosome elimination. Dwarf male sterile wheat (DMSW) and corn inducer are potential important germplasm for wheat breeding by recurrent selection and doubled haploid strategies.
Zhang, Wei +6 more
openaire +2 more sources
Advanced Science, EarlyView.DUET‐seq is an open‐source droplet platform that jointly profiles chromatin accessibility and gene expression from the same nucleus. Dissolvable dual‐linker hydrogel beads and a one‐step intra‐droplet RT‐PCR co‐index both modalities in under 12 h at roughly $0.04 per cell.
Dong Cheng +16 more
wiley +1 more source
Present status of haploidy research in onion (Allium cepa) – A review
The Indian Journal of Agricultural Sciences, 2019 Research on haploid induction and their subsequent utilization in basic and genomic research and breeding is still at its infancy due to paucity of research efforts, difficulties in various critical steps, large genome size and limited publications ...
ANIL KHAR +4 more
doaj +1 more source