Results 31 to 40 of about 36,717 (236)

A reporter screen in a human haploid cell line identifies CYLD as a constitutive inhibitor of NF-κB. [PDF]

PLoS ONE, 2013
The development of forward genetic screens in human haploid cells has the potential to transform our understanding of the genetic basis of cellular processes unique to man. So far, this approach has been limited mostly to the identification of genes that
Clarissa C Lee, Jan E Carette, Thijn R Brummelkamp, Hidde L Ploegh   +3 more
doaj   +1 more source

A novel visual marker to distinguish haploids from doubled haploids in rice (Oryza sativa, L) at early growth stages

Plant Methods, 2023
Doubled haploid technology, which enables the generation of homozygous lines in a single step, is one of the modern tools being employed for accelerating breeding processes in different crops.
Chaitanya Ghalagi, Malavalli Rajashekar Namratha, Kavita Kotyal, Shiva Prakash, Basavaiah Mohan Raju   +4 more
doaj   +1 more source

Recovery and characterization of a Citrus clementina Hort. ex Tan. 'Clemenules' haploid plant selected to establish the reference whole Citrus genome sequence

BMC Plant Biology, 2009
Background In recent years, the development of structural genomics has generated a growing interest in obtaining haploid plants. The use of homozygous lines presents a significant advantage for the accomplishment of sequencing projects. Commercial citrus
Navarro Luis, Ollitrault Patrick, Pina José A, Hernández María, Juárez José, Aleza Pablo   +5 more
doaj   +1 more source

Discrimination of haploid and diploid maize kernels via multispectral imaging [PDF]

, 2017
The use of doubled haploids (DHs) in maize has become ubiquitous in maize breeding programmes as it allows breeders to go from cross to evaluation in as little as 2 years. Two important aspects of the in vivo DH system used in maize are as follows: (i)
Michael A. Edberg, Carstensen, Jens Michael, Lübberstedt, Thomas, Gerald N. De La Fuente, Carstensen, Jens, Adsetts Edberg Hansen, Michael, Jens Michael Carstensen, De La Fuente, Gerald N., De La Fuente, Gerald, Thomas Lü bberstedt, Edberg, Michael   +10 more
core   +1 more source

Method for Identifying Maize Haploid Seeds by Applying Diffuse Transmission Near-Infrared Spectroscopy

, 2017
The identification for haploid seeds is an important process in maize haploid breeding. Thanks to the diffuse transmission (DT) technology of near-infrared (NIR) spectroscopy, maize haploid seeds can be selected automatically using NIR spectrum features.
Lina Yu, Jianchu Lin, Weijun Li, Hong Qin   +3 more
core   +1 more source

Heterozygous loss‐of‐function alleles associate the conserved 3′‐5′ exoribonuclease EXOSC10 with hypersensitivity to the anticancer drug 5‐fluorouracil

Molecular Oncology, EarlyView.
EXOSC10, an essential nuclear RNA exosome‐associated 3′‐5′ exoribonuclease, is inhibited by the anticancer drug 5‐fluorouracil (5‐FU), and EXOSC10 depletion increases 5‐FU sensitivity. The colon‐cancer variant EXOSC10S402T, located in a proteolysis motif, is stable and nuclear but nonfunctional in vivo.
Radhika Sain, Yann Le Page, Frédéric Percevault, Emmanuelle Becker, Luc Negroni, Almudena Fernandez, Marta Cantero, Diego Muñoz‐Santos, Lluís Montoliu, Cyrille Garnier, Michael Primig   +10 more
wiley   +1 more source

Protocols for In Vivo Doubled Haploid (DH) Technology in Maize Breeding: From Haploid Inducer Development to Haploid Genome Doubling

, 2022
Doubled haploid (DH) technology reduces the time required to obtain homozygous genotypes and accelerates plant breeding among other advantages. It is established in major crop species such as wheat, barley, maize, and canola.
Foster, Tyler, Aboobucker, Siddique I., Jubery, Talukder Z., Ganapathysubramanian, Baskar, Frei, Ursula K., Chen, Yu-Ru, Lubberstedt, Thomas   +6 more
core  

PSMA8‐Containing 20S Proteasome Regulates Spermiogenesis and Male Fertility

Advanced Science, EarlyView.
PSMA8 assembles s20S proteasome that degrades specific substrates in elongating spermatids. Degradation of s20S‐substrates activates translation of FXR1‐target mRNAs, which are essential for mitochondrial sheath formation and sperm morphogenesis.
Huiwen Cao, Qianting Zhang, Wei Xu, Anxuan Fang, Haomang Xu, Cheng Qiu, Lugeng He, Chao Yu   +7 more
wiley   +1 more source

Point Mutations in Centromeric Histone Induce Post-zygotic Incompatibility and Uniparental Inheritance. [PDF]

PLoS Genetics, 2015
The centromeric histone 3 variant (CENH3, aka CENP-A) is essential for the segregation of sister chromatids during mitosis and meiosis. To better define CENH3 functional constraints, we complemented a null allele in Arabidopsis with a variety of mutant ...
Sundaram Kuppu, Ek Han Tan, Hanh Nguyen, Andrea Rodgers, Luca Comai, Simon W L Chan, Anne B Britt   +6 more
doaj   +1 more source

DNA Replication Errors Drive Genome‐Wide Small Inverted Triplication Dynamics

Advanced Science, EarlyView.
This study provides insight into the dynamic equilibrium mechanism of a novel structural variant, small inverted triplication (SIT), which is generated by misalignment of the 3’ flap generated under DNA replication stress with palindromic sequence. Alternatively, the end sequence may fold back on itself to form an inverted fragment.
Yi Lei, Yu Zhou, Haitao Sun, Hang Yuan, Xinyu Pei, Jessica D. Hess, Yao Yan, Zunsong Hu, Mian Zhou, Zhaohui Gu, Li Zheng, Xiwei Wu, Binghui Shen   +12 more
wiley   +1 more source