Results 31 to 40 of about 35,284 (218)

Vector Integration Sites Identification for Gene-Trap Screening in Mammalian Haploid Cells [PDF]

open access: yesScientific Reports, 2017
AbstractForward genetic screens using retroviral (or transposon) gene-trap vectors in a haploid genome revolutionized the investigation of molecular networks in mammals. However, the sequencing data generated by Phenotypic interrogation followed by Tag sequencing (PhiT-seq) were not well characterized.
Yu, Jian   +1 more
openaire   +4 more sources

A Saccharomyces eubayanus haploid resource for research studies

open access: yesScientific Reports, 2022
Since its identification, Saccharomyces eubayanus has been recognized as the missing parent of the lager hybrid, S. pastorianus. This wild yeast has never been isolated from fermentation environments, thus representing an interesting candidate for ...
Jennifer Molinet   +9 more
doaj   +1 more source

A reporter screen in a human haploid cell line identifies CYLD as a constitutive inhibitor of NF-κB. [PDF]

open access: yesPLoS ONE, 2013
The development of forward genetic screens in human haploid cells has the potential to transform our understanding of the genetic basis of cellular processes unique to man. So far, this approach has been limited mostly to the identification of genes that
Clarissa C Lee   +3 more
doaj   +1 more source

Maize Haploid Identification via LSTM-CNN and Hyperspectral Imaging Technology

open access: yesCoRR, 2018
Accurate and fast identification of seed cultivars is crucial to plant breeding, with accelerating breeding of new products and increasing its quality. In our study, the first attempt to design a high-accurate identification model of maize haploid seeds from diploid ones based on optimum waveband selection of the LSTM-CNN algorithm is realized via deep
Xuan-Yu Wang   +3 more
openaire   +2 more sources

A novel visual marker to distinguish haploids from doubled haploids in rice (Oryza sativa, L) at early growth stages

open access: yesPlant Methods, 2023
Doubled haploid technology, which enables the generation of homozygous lines in a single step, is one of the modern tools being employed for accelerating breeding processes in different crops.
Chaitanya Ghalagi   +4 more
doaj   +1 more source

A versatile genetic tool: haploid cells

open access: yesStem Cell Research & Therapy, 2017
Haploid cells are excellent tools to study gene function as they contain a single copy of the genome and are thus unable to mask the effect of mutations.
Yanni Li, Ling Shuai
doaj   +1 more source

Recovery and characterization of a Citrus clementina Hort. ex Tan. 'Clemenules' haploid plant selected to establish the reference whole Citrus genome sequence

open access: yesBMC Plant Biology, 2009
Background In recent years, the development of structural genomics has generated a growing interest in obtaining haploid plants. The use of homozygous lines presents a significant advantage for the accomplishment of sequencing projects. Commercial citrus
Navarro Luis   +5 more
doaj   +1 more source

Discovery of gene regulatory elements through a new bioinformatics analysis of haploid genetic screens.

open access: yesPLoS ONE, 2019
The systematic identification of regulatory elements that control gene expression remains a challenge. Genetic screens that use untargeted mutagenesis have the potential to identify protein-coding genes, non-coding RNAs and regulatory elements, but their
Bhaven B Patel   +5 more
doaj   +1 more source

Identification of QTL underlying the main stem related traits in a doubled haploid barley population

open access: yesFrontiers in Plant Science, 2022
Lodging reduces grain yield in cereal crops. The height, diameter and strength of stem are crucial for lodging resistance, grain yield, and photosynthate transport in barley. Understanding the genetic basis of stem benefits barley breeding. Here, we evaluated 13 stem related traits after 28 days of heading in a barley DH population in two consecutive ...
Anyong Zhang   +11 more
openaire   +3 more sources

Are stomatal area and stomatal density reliable traits for identification of doubled haploids in maize?

open access: yesCrop Breeding and Applied Biotechnology, 2022
Abstract To meet the demands of the production chain, breeding programs need to reduce the time for selection and development of higher yielding maize genotypes. This involves application of new strategies, such as the use of the doubled haploid lines. We evaluated the use of stomatal area and density in distinguishing doubled haploids (DH) from false ...
Mariana Rodrigues Ribeiro   +7 more
openaire   +3 more sources

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