Results 21 to 30 of about 37,107 (214)

Identification and Expression Analysis of the Goji Haploid-Inducible Gene DMP. [PDF]

Int J Mol Sci
Goji, a plant unique to China, is recognized for its dual use as both a food and a medicine and is rich in various nutrients. However, long-term asexual propagation often leads to cultivar degeneration and viral accumulation, which severely impact its yield, quality, and disease resistance.
Yang Z, Wang C, Wang Z, Wu J.
europepmc   +2 more sources

A two-stage screening approach integrated with GWAS for rare phenotypes such as spontaneous haploid genome doubling in maize [PDF]

Frontiers in Plant Science
Spontaneous haploid genome doubling (SHGD) is a valuable trait in maize breeding, enabling the development of doubled haploid (DH) lines without chemical chromosome doubling.
Mercy Fakude, Tyler L. Foster, Yu-Ru Chen, Ann Murithi, Siddique Imran Aboobucker, Ursula Karoline Frei, Philip M. Dixon, Thomas Lübberstedt   +7 more
doaj   +2 more sources

Establishment of an efficient haploid identification system by engineering anthocyanin accumulation in the wheat embryo. [PDF]

Plant Commun, 2023
Qi X, Guo S, Zhong Y, Chen B, Liu Z, Yan T, Feng B, Ni Z, Chen S, Liu C.   +9 more
europepmc   +3 more sources

Targeted CENH3 protein depletion in egg cells enables highly efficient haploid induction [PDF]

Plant Communications
Doubled haploid technology is a transformative tool for accelerating plant breeding by enabling the rapid development of homozygous lines. While manipulation of the centromere-specific histone H3 variant CENH3 has been shown to induce haploids in ...
Saravanakumar Somasundaram, Seda Yaşar, Jörg Fuchs, Maria Cuacos, Julian Claassen, Oda Weiss, Andriy Kochevenko, Jonathan C. Lamb, Tengyu Li, Niklas Capdeville, Holger Puchta, Andreas Houben   +11 more
doaj   +2 more sources

HaSAPPy: A tool for candidate identification in pooled forward genetic screens of haploid mammalian cells.

PLoS Computational Biology, 2018
Haploid cells are increasingly used for screening of complex pathways in animal genomes. Hemizygous mutations introduced through viral insertional mutagenesis can be directly selected for phenotypic changes.
Giulio Di Minin, Andreas Postlmayr, Anton Wutz   +2 more
doaj   +6 more sources

Double-fluorescent proteins enable robust maternal haploid identification in wheat. [PDF]

aBIOTECH
Li Y, Dong L, Liu S, Sun G, Deng Z, Che Y, Cui D, Chen G, Zou X, Xu W, Kong X, Ma J, Mao L, Li A, Xie C, Geng S.   +15 more
europepmc   +2 more sources

RNAi in the Rhizarian Phytopathogen Plasmodiophora brassicae: The Causal Agent of Clubroot Disease in Cruciferous Crops. [PDF]

Adv Sci (Weinh)
This study uncovers an unusual RNAi pathway in the rhizarian pathogen Plasmodiophora brassicae. In the absence of Dicer, a Drosha‐like RNase III protein supports the biogenesis of predominant 21‐nt small RNAs, and two Argonaute proteins mediate small RNA‐guided silencing.
Zhang X, Wu Y, Hou J, Gao L, Pan L, Shen C, Zhao C, Gao F, Zhang Y, Ren L, Fang X, Dun X, Liu S, Liu L.   +13 more
europepmc   +2 more sources

Identification of DMP Family Members in Solanaceous Vegetables Potentially Involved in Haploid Induction

Horticulturae
Haploid breeding technology offers a promising means of significantly shortening the breeding cycle by rapidly generating homozygous inbred lines. Previous studies have shown that DMP8 is involved in haploid induction across various plant species.
Xuan Deng, Wenjian Zhong, Bo Liu, Xinyan Shen, Zhiyong Ren, Yongen Lu, Xin Wang, Bo Ouyang   +7 more
doaj   +2 more sources

Comprehensive CRISPR/Cas9-based mutagenesis identifies single-amino acid substitutions that abrogate SPEN function in X inactivation [PDF]

Nature Communications
While genetic screens have facilitated the dissection of protein function in animal development, advances in systematic point mutagenesis open new opportunities for forward genetics in mammalian cells. Here, we develop a CRISPR/Cas9-mediated base editing
Corinne Kaufmann, Sarah Sting, Chao Dai, Anton Wutz   +3 more
doaj   +2 more sources

Development of Specific Molecular and Phenotypic Marker-Based Haploid Inducers in Rice

Agronomy, 2023
Doubled haploid (DH) technology is an efficient strategy for producing completely homozygous lines for breeding programs. Mutations in the MATRILINEAL (MTL) phospholipase trigger intraspecific haploid induction in cereals.
Jian Wang, Huijing Yan, Xiaozhen Jiao, Jun Ren, Fengyue Hu, Huan Liang, Weihong Liang, Chaolei Liu   +7 more
doaj   +1 more source