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Haploid identification in maize [PDF]

open access: yesFrontiers in Plant Science
Doubled haploid (DH) line production through in vivo maternal haploid induction is widely adopted in maize breeding programs. The established protocol for DH production includes four steps namely in vivo maternal haploid induction, haploid identification,
Abil Dermail   +8 more
doaj   +4 more sources

Development of high-oil maize haploid inducer with a novel phenotyping strategy

open access: yesCrop Journal, 2022
Doubled haploid (DH) technology is important in modern maize breeding. Haploid inducers determine the efficiency of both haploid induction and identification.
Chenxu Liu   +9 more
doaj   +3 more sources

Advances in doubled haploid technology for rice breeding: mechanisms, applications, and future perspectives [PDF]

open access: yesFrontiers in Plant Science
Production of homozygous inbred lines is crucial in rice breeding programs. Doubled haploid (DH) technology enables the efficient development of pure lines and significantly shortens the breeding cycle compared to conventional methods.
Yiling Lin   +7 more
doaj   +2 more sources

Testcross Analysis of Pl-1 Marker Expression and Seedling Vigor in Thai Maize Germplasm for Doubled Haploid Breeding Applications [PDF]

open access: yesPlants
Doubled haploid (DH) technology accelerates maize breeding by generating completely homozygous lines within two generations, but its efficiency depends on reliable haploid identification markers.
Arnat Thawarorit   +6 more
doaj   +2 more sources

A two-stage screening approach integrated with GWAS for rare phenotypes such as spontaneous haploid genome doubling in maize [PDF]

open access: yesFrontiers in Plant Science
Spontaneous haploid genome doubling (SHGD) is a valuable trait in maize breeding, enabling the development of doubled haploid (DH) lines without chemical chromosome doubling.
Mercy Fakude   +7 more
doaj   +2 more sources

Targeted CENH3 protein depletion in egg cells enables highly efficient haploid induction [PDF]

open access: yesPlant Communications
Doubled haploid technology is a transformative tool for accelerating plant breeding by enabling the rapid development of homozygous lines. While manipulation of the centromere-specific histone H3 variant CENH3 has been shown to induce haploids in ...
Saravanakumar Somasundaram   +11 more
doaj   +2 more sources

Accelerating haploid induction rate and haploid validation through marker-assisted selection for qhir1 and qhir8 in maize

open access: yesFrontiers in Plant Science
Doubled haploid (DH) technology becomes more routinely applied in maize hybrid breeding. However, some issues in haploid induction and identification persist, requiring resolution to optimize DH production.
Kanogporn Khammona   +11 more
doaj   +3 more sources

Genes and genetics belong to maize haploid induction [PDF]

open access: yesFrontiers in Plant Science
Maize (Zea mays L.) is a globally significant cereal crop with diverse food, feed, and industry uses. The rapid development of homozygous inbred lines via double haploid (DH) technology has revolutionized maize breeding, reducing the time and cost ...
Kanogporn Khammona   +9 more
doaj   +2 more sources

Comprehensive CRISPR/Cas9-based mutagenesis identifies single-amino acid substitutions that abrogate SPEN function in X inactivation [PDF]

open access: yesNature Communications
While genetic screens have facilitated the dissection of protein function in animal development, advances in systematic point mutagenesis open new opportunities for forward genetics in mammalian cells. Here, we develop a CRISPR/Cas9-mediated base editing
Corinne Kaufmann   +3 more
doaj   +2 more sources

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