Results 31 to 40 of about 3,218 (171)

Ancient evolutionary trade-offs between yeast ploidy states. [PDF]

PLoS Genetics, 2013
The number of chromosome sets contained within the nucleus of eukaryotic organisms is a fundamental yet evolutionarily poorly characterized genetic variable of life.
Enikö Zörgö, Karolina Chwialkowska, Arne B Gjuvsland, Elena Garré, Per Sunnerhagen, Gianni Liti, Anders Blomberg, Stig W Omholt, Jonas Warringer   +8 more
doaj   +1 more source

Polycomb-mediated repression of paternal chromosomes maintains haploid dosage in diploid embryos of Marchantia

eLife, 2022
Complex mechanisms regulate gene dosage throughout eukaryotic life cycles. Mechanisms controlling gene dosage have been extensively studied in animals, however it is unknown how generalizable these mechanisms are to diverse eukaryotes.
Sean Akira Montgomery, Tetsuya Hisanaga, Nan Wang, Elin Axelsson, Svetlana Akimcheva, Milos Sramek, Chang Liu, Frédéric Berger   +7 more
doaj   +1 more source

Haploidy and aneuploidy in switchgrass mediated by misexpression of CENH3

The Plant Genome, 2023
Cross bred species such as switchgrass may benefit from advantageous breeding strategies requiring inbred lines. Doubled haploid production methods offer several ways that these lines can be produced that often involve uniparental genome elimination as ...
Sangwoong Yoon, Jennifer Bragg, Sheyla Aucar‐Yamato, Lisa Chanbusarakum, Kurtis Dluge, Prisca Cheng, Eduardo Blumwald, Yong Gu, Christian M. Tobias   +8 more
doaj   +1 more source

Haploidy in somatic cells is induced by mature oocytes in mice

Communications Biology, 2022
Yeonmi Lee, Aysha Trout, Nuria Marti-Guiterrez et al. examine different aspects of somatic cell haploidization in mouse enucleated oocytes. Their results provide further insight into generating oocytes carrying somatic genomes.
Yeonmi Lee, Aysha Trout, Nuria Marti-Gutierrez, Seoon Kang, Philip Xie, Aleksei Mikhalchenko, Bitnara Kim, Jiwan Choi, Seongjun So, Jongsuk Han, Jing Xu, Amy Koski, Hong Ma, Junchul David Yoon, Crystal Van Dyken, Hayley Darby, Dan Liang, Ying Li, Rebecca Tippner-Hedges, Fuhua Xu, Paula Amato, Gianpiero D. Palermo, Shoukhrat Mitalipov, Eunju Kang   +23 more
doaj   +1 more source

Haploidy of somatic cells in mouse oocyte using somatic cell nuclear transfer

Journal of Animal Reproduction and Biotechnology, 2022
Haploidization in somatic cells is the process of reducing the diploid somatic chromosomes to haploid. Several studies have attempted somatic haploidization using oocytes in mice and humans.
Yeonmi Lee , Eunju Kang
doaj   +1 more source

The prospects for using haploinducers in maize breeding

Биотехнология и селекция растений, 2020
The discovery of spontaneous haploid plants and the development of ways to produce them in in vitro culture have set a new direction important for breeding and for theoretical research in reproductive biology.
G. M. Asadova, A. V. Ulyanov, A. V. Karlov, E. B. Khatefov   +3 more
doaj   +1 more source

Effects of Antimitotic Agents, Temperature and Immersion Time on Chromosome Doubling Efficiency in Supersweet Corn (Zea mays L. var. saccharata)

Plant Breeding, EarlyView.
ABSTRACT Doubled haploid technology accelerates the development of fully homozygous maize lines compared with conventional selfing. However, specific protocols for supersweet corn (Zea mays L. var. saccharata), particularly regarding immersion time in antimitotic solutions and temperature during chromosome doubling, remain poorly defined.
Vitor Joaquim de Lucena, Juliana Moraes Machado de Oliveira, Bruno Figueiró Fregonezi, Iran de Azevedo Duarte, João Otávio Gonçalves dos Santos, Maria Eduarda Alves de Oliveira, Lucas Alves Camilo, Josué Maldonado Ferreira   +7 more
wiley   +1 more source

Genomic Selection for Haploid Induction Rate in Tropical Maize (Zea mays L.) Haploid Inducer Populations: Insights From Heterotic Pools and Recurrent Selection Cycles

Plant Breeding, EarlyView.
ABSTRACT Doubled‐haploid (DH) technology based on in vivo haploid induction has become an effective strategy for accelerating maize breeding. However, improving haploid inducer populations remains challenging because accurate estimation of haploid induction rate (HIR) requires labour‐intensive phenotypic validation, while putative HIR estimates are ...
Antônio Cláudio dos Santos Pinto, Jesimiel da Silva Viana, Felipe Sabadin, Roberto Fritsche‐Neto, Júlio César DoVale   +4 more
wiley   +1 more source

Resistance of Botrytis cinerea to anilinopyrimidine fungicides: A novel ARMS‐PCR method for the detection of Bcpos5 mutations and characterization of resistance using CRISPR/Cas9 editing

Pest Management Science, Volume 82, Issue 9, Page 8189-8198, September 2026.
A TETRA‐primer amplification refractory mutation system polymerase chain reaction (T‐ARMS‐PCR) method was developed for simultaneous detection of the L412F/V and G408V mutations. L412F/V mutants were created using the CRISPR/Cas tool and no fitness cost was observed.
Georgios Sofianos, Athanasios Petmezas, Anastasios Samaras, George Karaoglanidis   +3 more
wiley   +1 more source

The power of many: when genetics met yeasts and high‐throughput

Biological Reviews, Volume 101, Issue 4, Page 1662-1682, August 2026.
ABSTRACT In recent years, complex technological capabilities have evolved, driven by the need to solve complex and integrative biological questions through global analyses. New equipment allows the scaling up and automation of processes which previously were carried out on a very limited scale.
Víctor A. Tallada, Víctor Carranco
wiley   +1 more source