Results 61 to 70 of about 110,403 (278)

Optimized design parameters for CRISPR Cas9 and Cas12a homology-directed repair

Scientific Reports, 2021
CRISPR–Cas proteins are RNA-guided nucleases used to introduce double-stranded breaks (DSBs) at targeted genomic loci. DSBs are repaired by endogenous cellular pathways such as non-homologous end joining (NHEJ) and homology-directed repair (HDR ...
Mollie S. Schubert, Bernice Thommandru, Jessica Woodley, Rolf Turk, Shuqi Yan, Gavin Kurgan, Matthew S. McNeill, Garrett R. Rettig   +7 more
doaj   +1 more source

Construction of pathogenic Sec16a mutation mouse model using CRISPR/Cas9

Animal Models and Experimental Medicine, EarlyView.
Yaqiang Hu et al. engineered a pathogenic Sec16a mutant mouse model using CRISPR/Cas9 technology. They observed that the Sec16a mutant mice displayed diminished learning and memory capabilities, along with a limb‐clasping phenotype upon tail suspension.
Yaqiang Hu, Zhiyang Zeng, Xinyu Ming, Shuming Yin, Yuting Guan, Liangcai Gao, Dali Li   +6 more
wiley   +1 more source

Effects of glaucoma and snoring on cerebral oxygenation in the visual cortex: a study using functional Near Infrared Spectroscopy (fNIRS) [PDF]

, 2018
Purpose: The purpose of this study was to investigate the effects of snoring and glaucoma on the visual Haemodynamic Response (HDR) using functional Near Infrared Spectroscopy (fNIRS).
Aitchison, Ross T., Hill, Gemma, Imrie, Jayne, Kennedy, Graeme J., Mansfield, David, Morrison, Gordon, Shahani, Uma, Simmers, Anita J., Ward, Laura M.   +8 more
core   +1 more source

Zebrafish and CRISPR—A synergistic approach to decipher and cure human diseases

Animal Models and Experimental Medicine, EarlyView.
Zebrafish, with high genetic homology to humans, serves as a powerful vertebrate model for disease modeling and drug discovery. Integration of CRISPR/Cas9 technology enables precise genome editing, facilitating the development of translational models for human diseases.
Manikandan Sivaprakasam, Aashika Raagavi Jeanpierre, Salma Mohammed, Rajesh Srinivasan, Aman Kumar Mohanty   +4 more
wiley   +1 more source

HDR [PDF]

Cliopsy, 2021
openaire   +3 more sources

Pancreatic β-Cell Dysfunction in Diet-Induced Obese Mice: Roles of AMP-Kinase, Protein Kinase Cε, Mitochondrial and Cholesterol Metabolism, and Alterations in Gene Expression. [PDF]

PLoS ONE, 2016
Diet induced obese (DIO) mice can be stratified according to their weight gain in response to high fat diet as low responders (LDR) and high responders (HDR).
Émilie Pepin, Anfal Al-Mass, Camille Attané, Kezhuo Zhang, Julien Lamontagne, Roxane Lussier, S R Murthy Madiraju, Erik Joly, Neil B Ruderman, Robert Sladek, Marc Prentki, Marie-Line Peyot   +11 more
doaj   +1 more source

The Disk Population of the Taurus Star-Forming Region

, 2010
We have analyzed nearly all images of the Taurus star-forming region at 3.6-24um that were obtained during the cryogenic mission of the Spitzer Space Telescope (46 deg^2) and have measured photometry for all known members of the region that are within ...
Allen, Andrews, André, Backman, Balona, Baraffe, Barrado y Navascués, Beck, Bontemps, Bourke, Briceño, Briceño, Briceño, Briceño, Brown, Burgasser, C. Espaillat, Calvet, Carpenter, Carpenter, Chabrier, Cruz, Cruz, Currie, Currie, Currie, D'Alessio, Dahm, Dahm, Damjanov, Dobashi, Downes, Duchêne, Dunham, D’Alessio, Ercolano, Espaillat, Espaillat, Espaillat, Espaillat, Evans, Fazio, Feigelson, Flaherty, Forrest, Furlan, Furlan, Furlan, Gautier, Gizis, Gutermuth, Gutermuth, Haisch, Hartigan, Hartmann, Hartmann, Herczeg, Hernández, Hernández, Hernández, Hernández, Hughes, Hughes, Ireland, K. L. Luhman, Kenyon, Kenyon, Kenyon, Kim, Kirkpatrick, Kirkpatrick, Kraus, L. Hartmann, Lada, Lada, Looper, Luhman, Luhman, Luhman, Luhman, Luhman, Luhman, Luhman, Luhman, Luhman, Luhman, Luhman, Luhman, Luhman, Luhman, Luhman, Luhman, Luhman, Malfait, Mamajek, Mamajek, Marengo, Martín, Martín, Massarotti, Megeath, Megeath, Moitinho, Morrow, Motte, Motte, Muench, Muzerolle, N. Calvet, P. R. Allen, Prato, Preibisch, Prusti, Quillen, Rebull, Rieke, Rieke, Sicilia-Aguilar, Sicilia-Aguilar, Sicilia-Aguilar, Skrutskie, Slesnick, Song, Steffen, Walter, Watson, Webb, Werner, White, White, White, Wichmann, Zasowski   +132 more
core   +1 more source

Nonviral Gold Nanoparticle‐Mediated Delivery of CRISPR‐Cas9 Ribonucleoprotein and Long DNA Transgenes Into Primary Blood Cells

Advanced NanoBiomed Research, EarlyView.
CRISPR/Cas9 has revolutionized the field of gene therapy, but delivery remains an outstanding issue. We propose a nonviral gold‐nanoparticle platform for co‐delivery of CRISPR/Cas9 ribonucleoprotein and long 2.1 kilobase dsDNA transgene constructs. This CRISPR‐AuNP is inexpensive to produce and mediate gene editing and DNA delivery in T cells and CD34+
Rachel A. Cunningham, Karthikeya S. V. Gottimukkala, Daniel D. Lane, Katrina Poljakov, Patricia Lipson, Mark R. Enstrom, Alessandro Rizzi, Aude G. Chapuis, Jennifer E. Adair   +8 more
wiley   +1 more source

The Role of miRNAs in Chicken Immune Regulation and Prospects for Disease‐Resistant Breeding

Animal Research and One Health, EarlyView.
A schematic workflow illustrating the screening of disease‐resistant miRNAs and the generation of miRNA‐based disease‐resistant chickens via PGC‐mediated germline genome editing. ABSTRACT MicroRNAs (miRNAs) are emerging as pivotal regulators of the immune system, playing a decisive role in shaping disease resistance in chicken.
Qiangzhou Wang, Weiran Wu, Qingqing Cai, Jiaxing Wang, Qi Gao, Muhammad Iftikhar, Yulin Bi, Hao Bai, Lihong Gu, Guobin Chang, Shihao Chen   +10 more
wiley   +1 more source

Réparer la nature, est-ce bien raisonnable ? [PDF]

Sciences, Eaux & Territoires, 2017
Un problème se pose au gestionnaire qui souhaite préserver ou réhabiliter une zone humide : quel état de cette zone en perpétuelle évolution choisir ? Ce parti pris n’est pas anodin : il implique des notions de mémoire, d’héritage, de patrimoine.
DEREX, Jean-Michel
doaj