Results 81 to 90 of about 685,387 (303)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

open access: yesFEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point   +7 more
wiley   +1 more source

PREDICTION OF TWO-PHASE FLOW BOILING CHARACTERESTICS IN MICROCHANNELS HEAT SINK BY ARTIFICIAL NEURAL NETWORK

open access: yesJournal of Engineering and Sustainable Development, 2017
The current study investigated flow boiling heat transfer, pressure drop in a copper multi-parallel microchannels heat sink using R134a as a working fluid.
Adnan A. Abdulrasool   +2 more
doaj  

Estimation of Sensible and Latent Heat Fluxes from Different Ecosystems Using the Daily-Scale Flux Variance Method

open access: yesAtmosphere
A daily-scale flux variance (FV) method, which employs low-frequency air temperature measurements, was assessed against eddy covariance (EC) measurements of sensible and latent heat fluxes at four sites representing grassland and cropland ecosystems. The
Yanhong Xie   +6 more
doaj   +1 more source

Multi‐omics and low‐input proteomics profiling reveals dynamic regulation driving pluripotency initiation in early mouse embryos

open access: yesFEBS Open Bio, EarlyView.
Mouse pre‐implantation development involves a transition from totipotency to pluripotency. Integrating transcriptomics, epigenetic profiling, low‐input proteomics and functional assays, we show that eight‐cell embryos retain residual totipotency features, whereas cytoskeletal remodeling regulated by the ubiquitin‐proteasome system drives progression ...
Wanqiong Li   +8 more
wiley   +1 more source

Assessment of Surface Heat Flux and Deep Fluid Degassing in Fracture‐Dominated Geothermal Zones in Taxkorgan, Xinjiang, Western China

open access: yesGeochemistry, Geophysics, Geosystems
Understanding surface heat flux is essential for evaluating geothermal system dynamics and resource potential, particularly in tectonically active but non‐volcanic regions. This study presents the first integrated assessment of surface heat flux in three
Yingchun Wang   +4 more
doaj   +1 more source

Application of the Critical Heat Flux Look-Up Table to Large Diameter Tubes

open access: yesScience and Technology of Nuclear Installations, 2013
The critical heat flux look-up table was applied to a large diameter tube, namely 67 mm inside diameter tube, to predict the occurrence of the phenomenon for both vertical and horizontal uniformly heated tubes. Water was considered as coolant.
M. El Nakla   +5 more
doaj   +1 more source

Heat flux measurements [PDF]

open access: yes
A new automated, computer controlled heat flux measurement facility is described. Continuous transient and steady-state surface heat flux values varying from about 0.3 to 6 MW/sq m over a temperature range of 100 to 1200 K can be obtained in the facility.
Liebert, Curt H., Weikle, Donald H.
core   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains

open access: yesFEBS Open Bio, EarlyView.
The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth   +6 more
wiley   +1 more source

Observations of soil heat flux at Pune using a heat flux plate

open access: yesMAUSAM, 1980
Measurements of soil heat flux were made over bare black cotton soil at Pune in the period June 1976 to February 1977, using a soil heat flux plate. Hourly values of soil heat flux from 00 to 24 LAT are presented for selected days typical of the monsoon, post-monsoon and winter seasons.
R. R. KELKAR, V. R. CHIVATE, R. C. DUBEY
openaire   +2 more sources

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