Results 271 to 280 of about 150,776 (304)

HEK293 cell line: A vehicle for the expression of recombinant proteins

Journal of Pharmacological and Toxicological Methods, 2005
The HEK cell line has been extensively used as an expression tool for recombinant proteins since it was generated over 25 years ago. Although of epithelial origin, its biochemical machinery is capable of carrying out most of the post-translational folding and processing required to generate functional, mature protein from a wide spectrum of both ...
Philip, Thomas, Trevor G, Smart
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PTPN6 regulates the cell-surface expression of TRPM4 channels in HEK293 cells

Pflügers Archiv - European Journal of Physiology, 2018
Transient receptor-potential, cation channel, subfamily M, member 4 (TRPM4) channels regulate a variety of physiological and pathological processes; however, their roles as functional channels under diverse conditions remain unclear. In this study, cytosolic protein tyrosine phosphatase non-receptor type 6 (PTPN6) interacted with TRPM4 channels.
Dong Kun Lee, Jung Yeon Park, Jae Cheal Yoo, Eun Hye Byun, Yeon-Ju Bae, Young-Sun Lee, Nammi Park, Dawon Kang, Jaehee Han, Jae Yong Park, Eunmi Hwang, Seong-Geun Hong   +11 more
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Interferon inhibitors increase rAAV production in HEK293 cells

Journal of Biotechnology
Recombinant adeno-associated viruses (rAAVs) comprise a promising viral vector for therapeutic gene delivery to treat disease. However, the current manufacturing capability of rAAVs must be improved to meet commercial demand. Previously published omics studies indicate that rAAV production through transient transfection triggers antiviral responses and
Yongdan, Wang, Qiang, Fu, Sha, Sha, Seongkyu, Yoon   +3 more
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Effect of Formaldehyde on Cellular Proliferation of HEK293 Cells

2007 1st International Conference on Bioinformatics and Biomedical Engineering, 2007
In order to investigate the influences of formaldehyde with different doses on the cellular proliferation and cell cycle of the HEK293 cells, methyl thiazol tetrazolium (MTT) assay and flow cytometry were applied to measure the cellular proliferation and cell cycle.
Xu Qian, He Hujun, Yang Guangtao, Yang Xu   +3 more
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Expression of Recombinant Human α-Glucosidase in HEK293 Cells

Journal of Agricultural and Food Chemistry
In mammals, intestinal α-glucosidase exists as a maltase-glucoamylase complex (MGAM) and a sucrase-isomaltase complex (SI). In this study, we transiently expressed human MGAM and SI in human embryonic kidney 293 (HEK293) cells. At pH 6.0 and 37 °C, the MGAM-expressing HEK293 cells extract (MGE) exhibited maltase, glucoamylase, and isomaltase activities
So Nishimoto, Anaïs Debarbat, Yuki Ikeda, Emi Arikawa, Yuki Odagaki, Haruna Yano, Ying Qiao, Masaaki Ito, Toshiyuki Kimura, Teisuke Takita, Kiyoshi Yasukawa   +10 more
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DIXDC1 co‐localizes and interacts with γ‐tubulin in HEK293 cells

Cell Biology International, 2009
AbstractDIXDC1 is a Dishevelled‐Axin (DIX) domain‐containing protein involved in neural development and Wnt signaling pathway. Besides the DIX domain, DIXDC1 also contains a coiled‐coil domain (MTH domain), which is a common feature of centrosomal proteins.
Yanrui, Wu, Xiaotang, Jing, Xin, Ma, Yan, Wu, Xuefeng, Ding, Wenhong, Fan, Ming, Fan   +6 more
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Dimethyl sulfoxide effects on hERG channels expressed in HEK293 cells

Journal of Pharmacological and Toxicological Methods, 2006
Dimethyl sulfoxide (DMSO) is widely used as a solvent to facilitate formulation of test substances in cell perfusion solutions. However, DMSO concentration in bath (extracellular) solution is usually limited to 0.1-0.3% to avoid DMSO-induced changes in cell morphology and membrane properties due to elevation of osmolality. The purpose of this study was
Xiaoyi, Du, Daniel, Lu, Eric D, Daharsh, Aizhen, Yao, Rebecca, Dewoody, Jian-An, Yao   +5 more
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The role of thiol reduction in hydroquinone-induced apoptosis in HEK293 cells

Chemico-Biological Interactions, 2003
Hydroquinone (HQ) is a chemical used as a reducing agent, antioxidant, polymerization inhibitor, and chemical intermediate. It has a minor use as a bleaching agent in dermatologic preparations. HQ also occurs as a main metabolite of benzene. In the present study, HQ-induced apoptosis was evaluated by cell morphology changes, determination of ...
Dong-Xiao, Shen, Xu, Shi, Juan-Ling, Fu, Ying-Mei, Zhang, Zong-Can, Zhou   +4 more
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Low-level arsenite induced gene expression in HEK293 cells

Toxicology, 2003
Chronic, low-level exposure to arsenic frequently results in skin, lung, bladder, and kidney cancer. Since arsenic is primarily excreted via the kidney, this study focused on this target tissue. Gene array was used as a sensitive low-level monitor of the impact of arsenic on this target tissue.
Xing Hui, Zheng, George S, Watts, Skip, Vaught, A Jay, Gandolfi   +3 more
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Optimising the transient expression of GABA(A) receptors in adherent HEK293 cells

Protein Expression and Purification, 2019
Owing to their therapeutic relevance, considerable efforts are devoted to the structural characterisation of membrane proteins. Such studies are limited by the availability of high quality protein due to the difficulty of overexpression in recombinant mammalian systems. We sought to systematically optimise multiple aspects in the process of transiently
Steven O. Devenish, Graham A.R. Johnston, Mary Chebib, Jane R. Hanrahan   +3 more
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