Results 271 to 280 of about 4,870,076 (306)
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PTPN6 regulates the cell-surface expression of TRPM4 channels in HEK293 cells
Pflügers Archiv - European Journal of Physiology, 2018Transient receptor-potential, cation channel, subfamily M, member 4 (TRPM4) channels regulate a variety of physiological and pathological processes; however, their roles as functional channels under diverse conditions remain unclear. In this study, cytosolic protein tyrosine phosphatase non-receptor type 6 (PTPN6) interacted with TRPM4 channels.
Dong Kun Lee +11 more
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Interferon inhibitors increase rAAV production in HEK293 cells
Journal of BiotechnologyRecombinant adeno-associated viruses (rAAVs) comprise a promising viral vector for therapeutic gene delivery to treat disease. However, the current manufacturing capability of rAAVs must be improved to meet commercial demand. Previously published omics studies indicate that rAAV production through transient transfection triggers antiviral responses and
Yongdan, Wang +3 more
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Effect of Formaldehyde on Cellular Proliferation of HEK293 Cells
2007 1st International Conference on Bioinformatics and Biomedical Engineering, 2007In order to investigate the influences of formaldehyde with different doses on the cellular proliferation and cell cycle of the HEK293 cells, methyl thiazol tetrazolium (MTT) assay and flow cytometry were applied to measure the cellular proliferation and cell cycle.
Xu Qian +3 more
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Expression of Recombinant Human α-Glucosidase in HEK293 Cells
Journal of Agricultural and Food ChemistryIn mammals, intestinal α-glucosidase exists as a maltase-glucoamylase complex (MGAM) and a sucrase-isomaltase complex (SI). In this study, we transiently expressed human MGAM and SI in human embryonic kidney 293 (HEK293) cells. At pH 6.0 and 37 °C, the MGAM-expressing HEK293 cells extract (MGE) exhibited maltase, glucoamylase, and isomaltase activities
So Nishimoto +10 more
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Experiences with HEK293: A Human Cell Line.
PDA journal of pharmaceutical science and technology, 2016The paper describes considerations taken with regard to a recombinant human cell line (HEK293) producing a therapeutic protein. When working with HEK293 cells, besides standard viral safety issues, special precautions should be taken because this cell line is susceptible to infection by numerous viruses.
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Subcellular localization of human neutral ceramidase expressed in HEK293 cells
Biochemical and Biophysical Research Communications, 2005We previously reported that rat and mouse neutral ceramidases were mainly localized to plasma membranes as a type II integral membrane protein and partly detached from the cells via processing of the N-terminal/anchor sequence when expressed in HEK293 cells [M. Tani, H. Iida, M.
Young-ha, Hwang +4 more
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Application of HyperTRIBE to UPF1 in HEK293 cells
The application of HyperTRIBE technology to identify target genes of the RNA-binding protein UPF1 in HEK293 cells. This study includes five RNA-seq samples: two biological replicates of UPF1-ADAR2cd, two biological replicates of mCherry-ADAR2cd, and one GFP sample.openaire +1 more source
DIXDC1 co‐localizes and interacts with γ‐tubulin in HEK293 cells
Cell Biology International, 2009AbstractDIXDC1 is a Dishevelled‐Axin (DIX) domain‐containing protein involved in neural development and Wnt signaling pathway. Besides the DIX domain, DIXDC1 also contains a coiled‐coil domain (MTH domain), which is a common feature of centrosomal proteins.
Yanrui, Wu +6 more
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Recombinant Production of Glycoengineered Mucins in HEK293-F Cells
Recombinant mucins are attractive polymeric building blocks for new biomaterials, biolubricants, and therapeutics. Advances in glycoengineered host cell systems now enable the recombinant production of mucins with tailored O-glycan side chains, offering new opportunities to tune the functionality of mucins and investigate the biology of specific O ...Ling-Ting, Huang +2 more
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Dimethyl sulfoxide effects on hERG channels expressed in HEK293 cells
Journal of Pharmacological and Toxicological Methods, 2006Dimethyl sulfoxide (DMSO) is widely used as a solvent to facilitate formulation of test substances in cell perfusion solutions. However, DMSO concentration in bath (extracellular) solution is usually limited to 0.1-0.3% to avoid DMSO-induced changes in cell morphology and membrane properties due to elevation of osmolality. The purpose of this study was
Xiaoyi, Du +5 more
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