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Molecular characterization of HEK293 cells as emerging versatile cell factories
Current Opinion in Biotechnology, 2021HEK293 cell lines are used for the production of recombinant proteins, virus-like particles and viral vectors. Recent work has generated molecular (systems level) characterisation of HEK293 variants that has enabled re-engineering of the cells towards enhanced use for manufacture-scale production of recombinant biopharmaceuticals (assessment of 'safe ...
Pulix, Michela +3 more
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Functional Reconstruction of NLRs in HEK293 Cells
2016Inflammasomes are molecular complexes that initiate innate immune response. They are mainly expressed by immune cells; however, molecular manipulations in these cells remain very difficult. Here, we describe a simple protocol to overexpress and activate functional NRLP3 inflammasomes in HEK293 cells.
Compan, Vincent, Lopez-Castejon, Gloria
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Upregulation of PHLDA2 in Dicer knockdown HEK293 cells
Biochimica et Biophysica Acta (BBA) - General Subjects, 2007It has been reported that RNAi-dependent chromatin silencing in vertebrates is not restricted to the centromeres. To address whether RNAi machinery could regulate the chromatin structure of imprinted genes, we knocked down Dicer in HEK293 cells and found that the expression of PHLDA2, one of the several genes in the imprinted gene domain of 11p15.5 ...
Kai-Fu, Tang +9 more
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PIASy interacts with p73α and regulates cell cycle in HEK293 cells
Cellular Immunology, 2010p73, a recently described member of the p53 family of transcription factors, undergoes a number of posttranslational modifications, inducing cell cycle arrest and apoptosis. In the present study, we demonstrate that PIASy, a member of the PIAS SUMO-ligase family, interacts with p73alpha, and that PIASy-mediated sumoylation promotes proteasomal ...
Chao, Zhang +5 more
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Effects of Wnt proteins on cell proliferation and apoptosis in HEK293 cells
Cell Biology International, 2008AbstractWnt proteins and Wnt signalings have been implicated in a variety of development and cell processes, while aberrant activation of Wnt signaling is linked to a range of cancers in many tissues. In this study, we used the HEK293 cell line to investigate the effects of Wnt3a and Wnt5a on proliferation and apoptosis in a serum starvation culture ...
Liwei, Jia +3 more
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HEK293 cell line: A vehicle for the expression of recombinant proteins
Journal of Pharmacological and Toxicological Methods, 2005The HEK cell line has been extensively used as an expression tool for recombinant proteins since it was generated over 25 years ago. Although of epithelial origin, its biochemical machinery is capable of carrying out most of the post-translational folding and processing required to generate functional, mature protein from a wide spectrum of both ...
Philip, Thomas, Trevor G, Smart
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Transient Gene Expression in Suspension HEK293-EBNA1 Cells
2018Transient gene expression in human embryo kidney 293 (HEK293) cells is an established approach for the rapid production of large amounts of recombinant proteins (r-proteins). Milligram to gram quantities of r-proteins can be typically obtained within less than 10 days following transfection. In this chapter, we describe a simple and robust transfection
L’Abbé, Denis +4 more
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PTPN6 regulates the cell-surface expression of TRPM4 channels in HEK293 cells
Pflügers Archiv - European Journal of Physiology, 2018Transient receptor-potential, cation channel, subfamily M, member 4 (TRPM4) channels regulate a variety of physiological and pathological processes; however, their roles as functional channels under diverse conditions remain unclear. In this study, cytosolic protein tyrosine phosphatase non-receptor type 6 (PTPN6) interacted with TRPM4 channels.
Dong Kun Lee +11 more
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Effect of Formaldehyde on Cellular Proliferation of HEK293 Cells
2007 1st International Conference on Bioinformatics and Biomedical Engineering, 2007In order to investigate the influences of formaldehyde with different doses on the cellular proliferation and cell cycle of the HEK293 cells, methyl thiazol tetrazolium (MTT) assay and flow cytometry were applied to measure the cellular proliferation and cell cycle.
Xu Qian +3 more
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Interferon inhibitors increase rAAV production in HEK293 cells
Journal of BiotechnologyRecombinant adeno-associated viruses (rAAVs) comprise a promising viral vector for therapeutic gene delivery to treat disease. However, the current manufacturing capability of rAAVs must be improved to meet commercial demand. Previously published omics studies indicate that rAAV production through transient transfection triggers antiviral responses and
Yongdan, Wang +3 more
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