Results 261 to 270 of about 150,776 (304)
Spatiotemporal dynamics of β‐arrestin‐mediated Src activation in 5‐HT7 receptor signaling pathway
The FEBS Journal, EarlyView.GPCRs induce distinct cellular responses via G protein‐ or β‐arrestin‐mediated signaling pathways. This study revealed that β‐arrestin‐biased 5‐HT7R ligand induces slow, sustained Src activation, contrasting with transient G protein‐mediated activation.
Hyunbin Kim +8 more
wiley +1 more source
The FEBS Journal, EarlyView.A targeted phototoxic peptide selectively accumulates in cells expressing chemokine‐like receptor 1 (CMKLR1). Phototoxicity of the native peptide is low, but cleavage by lysosomal protease cathepsin B releases a 6‐tetramethylrhodamine (TMR) labeled nona‐ᴅ‐arginine which mediates lysosomal rupture and cell death upon irradiation.
Moritz List +2 more
wiley +1 more source
Upregulation of PHLDA2 in Dicer knockdown HEK293 cells
Biochimica et Biophysica Acta (BBA) - General Subjects, 2007 It has been reported that RNAi-dependent chromatin silencing in vertebrates is not restricted to the centromeres. To address whether RNAi machinery could regulate the chromatin structure of imprinted genes, we knocked down Dicer in HEK293 cells and found that the expression of PHLDA2, one of the several genes in the imprinted gene domain of 11p15.5 ...
Kai-Fu, Tang +9 more
core +5 more sources
Functional Reconstruction of NLRs in HEK293 Cells
, 2016 Inflammasomes are molecular complexes that initiate innate immune response. They are mainly expressed by immune cells; however, molecular manipulations in these cells remain very difficult. Here, we describe a simple protocol to overexpress and activate functional NRLP3 inflammasomes in HEK293 cells.
Compan, Vincent, Lopez-Castejon, Gloria
core +6 more sources
Transient Gene Expression in Suspension HEK293-EBNA1 Cells
, 2018 Transient gene expression in human embryo kidney 293 (HEK293) cells is an established approach for the rapid production of large amounts of recombinant proteins (r-proteins). Milligram to gram quantities of r-proteins can be typically obtained within less than 10 days following transfection. In this chapter, we describe a simple and robust transfection
L’Abbé, Denis +4 more
openaire +3 more sources
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Molecular characterization of HEK293 cells as emerging versatile cell factories
Current Opinion in Biotechnology, 2021HEK293 cell lines are used for the production of recombinant proteins, virus-like particles and viral vectors. Recent work has generated molecular (systems level) characterisation of HEK293 variants that has enabled re-engineering of the cells towards enhanced use for manufacture-scale production of recombinant biopharmaceuticals (assessment of 'safe ...
Pulix, Michela +3 more
openaire +5 more sources
Journal of General Physiology, 1999 Producción CientíficaVoltage-gated K+ (KV) channels are protein complexes composed of ion-conducting integral membrane α subunits and cytoplasmic modulatory β subunits.
M TERESA Pérez-García +2 more
exaly +2 more sources
PIASy interacts with p73α and regulates cell cycle in HEK293 cells
Cellular Immunology, 2010p73, a recently described member of the p53 family of transcription factors, undergoes a number of posttranslational modifications, inducing cell cycle arrest and apoptosis. In the present study, we demonstrate that PIASy, a member of the PIAS SUMO-ligase family, interacts with p73alpha, and that PIASy-mediated sumoylation promotes proteasomal ...
Chao, Zhang +5 more
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Effects of Wnt proteins on cell proliferation and apoptosis in HEK293 cells
Cell Biology International, 2008AbstractWnt proteins and Wnt signalings have been implicated in a variety of development and cell processes, while aberrant activation of Wnt signaling is linked to a range of cancers in many tissues. In this study, we used the HEK293 cell line to investigate the effects of Wnt3a and Wnt5a on proliferation and apoptosis in a serum starvation culture ...
Liwei, Jia +3 more
openaire +2 more sources