Results 281 to 290 of about 150,776 (304)
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Application of HyperTRIBE to UPF1 in HEK293 cells
The application of HyperTRIBE technology to identify target genes of the RNA-binding protein UPF1 in HEK293 cells. This study includes five RNA-seq samples: two biological replicates of UPF1-ADAR2cd, two biological replicates of mCherry-ADAR2cd, and one GFP sample.openaire +1 more source
Recombinant Production of Glycoengineered Mucins in HEK293-F Cells
Recombinant mucins are attractive polymeric building blocks for new biomaterials, biolubricants, and therapeutics. Advances in glycoengineered host cell systems now enable the recombinant production of mucins with tailored O-glycan side chains, offering new opportunities to tune the functionality of mucins and investigate the biology of specific O ...Ling-Ting, Huang +2 more
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Experiences with HEK293: A Human Cell Line.
PDA journal of pharmaceutical science and technology, 2016The paper describes considerations taken with regard to a recombinant human cell line (HEK293) producing a therapeutic protein. When working with HEK293 cells, besides standard viral safety issues, special precautions should be taken because this cell line is susceptible to infection by numerous viruses.
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High Throughput Transfection of HEK293 Cells for Transient Protein Production
2018Transient transfection of mammalian cells is used in the biotechnology industry to quickly supply recombinant protein for research and large molecule drug development. Here, we describe a method for high throughput transient transfection of Human Embryonic Kidney 293 (HEK293) cells in 30 mL tubespins using polyethylenimine (PEI) as a transfection ...
Tia A, Arena +2 more
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[Cloning and expression analysis of Sirt2 in HEK293 cells].
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology, 2010To construct eukaryotic expression vector of Sirt2 and detect its expression in HEK293 cells.Total RNA was isolated from brain tissue of adult SD rat. A 1 130 bp fragment containing the coding region of Sirt2 was amplified by RT-PCR and the resulting PCR product was subcloned into PMD20-T vector and sequenced.
Tao, Wang +4 more
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Engineering of the CMV promoter for controlled expression of recombinant genes in HEK293 cells
Biotechnology Journal, 2022Yusuf B Johari +2 more
exaly
Effect of single wall carbon nanotubes on human HEK293 cells
Toxicology Letters, 2005Daxiang Cui +2 more
exaly