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High Throughput Transfection of HEK293 Cells for Transient Protein Production

2018
Transient transfection of mammalian cells is used in the biotechnology industry to quickly supply recombinant protein for research and large molecule drug development. Here, we describe a method for high throughput transient transfection of Human Embryonic Kidney 293 (HEK293) cells in 30 mL tubespins using polyethylenimine (PEI) as a transfection ...
Tia A, Arena   +2 more
openaire   +2 more sources

[Cloning and expression analysis of Sirt2 in HEK293 cells].

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology, 2010
To construct eukaryotic expression vector of Sirt2 and detect its expression in HEK293 cells.Total RNA was isolated from brain tissue of adult SD rat. A 1 130 bp fragment containing the coding region of Sirt2 was amplified by RT-PCR and the resulting PCR product was subcloned into PMD20-T vector and sequenced.
Tao, Wang   +4 more
openaire   +1 more source

Stem Cell Epigenetics in Medical Therapy

Medical Epigenetics, 2021
Peng Jin, Liqi Shu
exaly  

HEK293 Cell Line as a Platform to Produce Recombinant Proteins and Viral Vectors

Frontiers in Bioengineering and Biotechnology, 2021
Say Kong Ng
exaly  

Engineering of the CMV Promoter for Controlled Expression of Recombinant Genes in HEK293 Cells

Biotechnology Journal, 2022
Yusuf B Johari   +2 more
exaly  

Effect of Single Wall Carbon Nanotubes on Human HEK293 Cells

Toxicology Letters, 2005
Cengiz Özkan, Furong Tian, Huajian Gao
exaly  

Transfection in HEK293 Cells

Karuppusamy Arunachalam   +1 more
openaire   +1 more source

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