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High Throughput Transfection of HEK293 Cells for Transient Protein Production
2018Transient transfection of mammalian cells is used in the biotechnology industry to quickly supply recombinant protein for research and large molecule drug development. Here, we describe a method for high throughput transient transfection of Human Embryonic Kidney 293 (HEK293) cells in 30 mL tubespins using polyethylenimine (PEI) as a transfection ...
Tia A, Arena +2 more
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[Cloning and expression analysis of Sirt2 in HEK293 cells].
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology, 2010To construct eukaryotic expression vector of Sirt2 and detect its expression in HEK293 cells.Total RNA was isolated from brain tissue of adult SD rat. A 1 130 bp fragment containing the coding region of Sirt2 was amplified by RT-PCR and the resulting PCR product was subcloned into PMD20-T vector and sequenced.
Tao, Wang +4 more
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HEK293 Cell Line as a Platform to Produce Recombinant Proteins and Viral Vectors
Frontiers in Bioengineering and Biotechnology, 2021Say Kong Ng
exaly
Engineering of the CMV Promoter for Controlled Expression of Recombinant Genes in HEK293 Cells
Biotechnology Journal, 2022Yusuf B Johari +2 more
exaly
Effect of Single Wall Carbon Nanotubes on Human HEK293 Cells
Toxicology Letters, 2005Cengiz Özkan, Furong Tian, Huajian Gao
exaly

