Identification of a novel nuclease activity in human DDX49 helicase
Royal Society Open ScienceHuman DDX49 is an emerging target in cancer progression and retroviral diseases through its essential roles in nucleolar RNA processing. Here, we identify nuclease activity of human DDX49, which requires active site aspartate residues within a conserved ...
Ashley J. Parkes +7 more
doaj +1 more source
New Insights Into DNA Helicases as Druggable Targets for Cancer Therapy
Frontiers in Molecular Biosciences, 2018 Small molecules that deter the functions of DNA damage response machinery are postulated to be useful for enhancing the DNA damaging effects of chemotherapy or ionizing radiation treatments to combat cancer by impairing the proliferative capacity of ...
Arindam Datta, Robert M. Brosh
doaj +1 more source
UPF1-like helicase grip on nucleic acids dictates processivity
Nature Communications, 2018 Helicases are molecular engines which translocate along nucleic acids (NA) to unwind double-strands or remodel NA–protein complexes. While they have an essential role in genome structure and expression, the rules dictating their processivity remain ...
Joanne Kanaan +5 more
semanticscholar +1 more source
Dynamic removal of replication protein A by Dna2 facilitates primer cleavage during Okazaki fragment processing in Saccharomyces cerevisiae [PDF]
, 2008 Eukaryotic Okazaki fragments are initiated by an RNA/DNA primer, which is removed before the fragments are joined. Polymerase d displaces the primer into a flap for processing.
Bambara, Robert A. +3 more
core +3 more sources
Rapid purification of helicase proteins and in vitro analysis of helicase activity [PDF]
Methods, 2010 Most processes involving an organism’s genetic material, including replication, repair and recombination, require access to single stranded DNA as a template or reaction intermediate. To disrupt the hydrogen bonds between the two strands in double stranded DNA, organisms utilize proteins called DNA helicases. DNA helicases use duplex DNA as a substrate
Steven W. Matson, Kambiz Tahmaseb
openaire +3 more sources
Crystal structure of the Ilheus virus helicase: implications for enzyme function and drug design
Cell & Bioscience, 2022 Background The Ilheus virus (ILHV) is an encephalitis associated arthropod-borne flavivirus. It was first identified in Ilheus City in the northeast Brazil before spreading to a wider geographic range.
De-Ping Wang +8 more
doaj +1 more source
Structural and Functional Studies of Chikungunya Virus nsP2
Proceedings, 2020 Chikungunya virus (CHIKV) is transmitted to humans through mosquitoes and causes Chikungunya fever. Nonstructural protein 2 (nsP2) contains an N-terminal RNA helicase with both nucleotide triphosphatase and RNA triphosphatase activities, and a C-terminal
Yee Song Law +10 more
doaj +1 more source
Primase is required for helicase activity and helicase alters the specificity of primase in the enteropathogen Clostridium difficile [PDF]
Open Biology, 2016 DNA replication is an essential and conserved process in all domains of life and may serve as a target for the development of new antimicrobials. However, such developments are hindered by subtle mechanistic differences and limited understanding of DNA ...
Erika van Eijk +8 more
doaj +1 more source
Structure of NS3 helicase from Zika virus
Nature Structural &Molecular Biology, 2016 Zika virus has emerged as a pathogen of major health concern. Here, we present a high-resolution (1.62-Å) crystal structure of the RNA helicase from the French Polynesia strain. The structure is similar to that of the RNA helicase from Dengue virus, with
R. Jain +3 more
semanticscholar +1 more source
Dna2p Helicase/Nuclease Is a Tracking Protein, Like FEN1, for Flap Cleavage during Okazaki Fragment Maturation [PDF]
, 2004 During cellular DNA replication the lagging strand is generated as discontinuous segments called Okazaki fragments. Each contains an initiator RNA primer that is removed prior to joining of the strands.
Bambara, Robert A. +2 more
core +1 more source