Results 121 to 130 of about 191,259 (287)

The Coronavirus helicase in replication

Virus Research
The coronavirus nonstructural protein (nsp) 13 encodes an RNA helicase (nsp13-HEL) with multiple enzymatic functions, including unwinding and nucleoside phosphatase (NTPase) activities. Attempts for enzymatic inactivation have defined the nsp13-HEL as a critical enzyme for viral replication and a high-priority target for antiviral development ...
Samantha L. Grimes, Mark R. Denison
openaire   +3 more sources

Mammalian DNA helicase

Nucleic Acids Research, 1985
A forked DNA was constructed to serve as a substrate for DNA helicases. It contains features closely resembling a natural replication fork. The DNA was prepared in large amounts and was used to assay displacement activity during isolation from calf thymus DNA polymerases alpha holoenzyme. One form of DNA polymerase alpha holoenzyme is possibly involved
Ulrich Hübscher, Hanspeter Stalder
openaire   +4 more sources

Protein cofactor competition regulates the action of a multifunctional RNA helicase in different pathways

RNA Biology, 2016
A rapidly increasing number of RNA helicases are implicated in several distinct cellular processes, however, the modes of regulation of multifunctional RNA helicases and their recruitment to different target complexes have remained unknown. Here, we show
A. Heininger, Philipp Hackert, Alexandra Z. Andreou, Kum-Loong Boon, Indira Memet, Mira Prior, A. Clancy, Bernhard Schmidt, H. Urlaub, E. Schleiff, K. Sloan, Markus Deckers, R. Lührmann, J. Enderlein, D. Klostermeier, P. Rehling, M. Bohnsack   +16 more
semanticscholar   +1 more source

In the absence of ATPase activity, pre-RC formation is blocked prior to MCM2-7 hexamer dimerization [PDF]

, 2013
The origin recognition complex (ORC) of Saccharomyces cerevisiae binds origin DNA and cooperates with Cdc6 and Cdt1 to load the replicative helicase MCM2–7 onto DNA. Helicase loading involves two MCM2–7 hexamers that assemble into a double hexamer around
A. Fernandez-Cid, A. Riera, Bell, Bowers, Bray, C. Evrin, C. Speck, Casas-Terradellas, Chen, Cocker, Coleman, Costa, Donovan, Dueber, Gillespie, Herbig, Holm, J. Zech, Klemm, Krupinski, Laemmli, Liang, Liang, Liu, M. C. Herrera, Mizushima, Neuwald, P. Clarke, Perkins, R. Lurz, Randell, Remus, Remus, Roy, S. Brill, Schepers, Seki, Singleton, Steven, Stoeber, Takara, Tanaka, Watanabe, Weinreich, Wendler, Williams, Ying, Zhang   +47 more
core   +1 more source

BLM helicase facilitates telomere replication during leading strand synthesis of telomeres

Journal of Cell Biology, 2015
BLM helicase facilitates telomere replication by resolving G-quadruplex structures that can form in the G-rich repeats during leading strand synthesis.
W. C. Drosopoulos, Settapong T. Kosiyatrakul, C. Schildkraut   +2 more
semanticscholar   +1 more source

DNA end resection by Dna2–Sgs1–RPA and its stimulation by Top3–Rmi1 and Mre11–Rad50–Xrs2 [PDF]

, 2010
The repair of DNA double-strand breaks (DSBs) by homologous recombination requires processing of broken ends. For repair to start, the DSB must first be resected to generate a 3′-single-stranded DNA (ssDNA) overhang, which becomes a substrate for the DNA
AV Nimonkar, B Llorente, BO Krogh, C Zierhut, DG Anderson, E Alani, E Hartsuiker, Elda Cannavo, EP Mimitou, FG Harmon, HI Kao, I Chiolo, J LeBowitz, JA Cobb, JA Solinger, JR Mullen, Judith L. Campbell, KH Bae, L Wu, M Chang, ME Budd, ME Budd, ME Budd, MJ Neale, MS Dillingham, N Handa, P Cejka, P Szankasi, Petr Cejka, Piotr Polaczek, S Gangloff, S Gravel, SH Bae, SH Bae, Stephen C. Kowalczykowski, Subhash Pokharel, T Masuda-Sasa, Taro Masuda-Sasa, TH Stracker, X Li, Z Zhu   +40 more
core   +4 more sources

Expression of catalytic mutants of the mtDNA helicase Twinkle and polymerase POLG causes distinct replication stalling phenotypes. [PDF]

, 2007
The mechanism of mitochondrial DNA replication is a subject of intense debate. One model proposes a strand-asynchronous replication in which both strands of the circular genome are replicated semi-independently while the other model proposes both a ...
Goffart, S, Pohjoismäki, JLO, Spelbrink, JN, Wanrooij, S, Yasukawa, T   +4 more
core   +1 more source

The crystal structure of Zika virus helicase: basis for antiviral drug design

Protein & Cell, 2016
The genus of Flavivirus contains important human pathogens, including dengue (DENV), yellow fever (YFV), West Nile (WNV), Japanese encephalitis (JEV), and tick-borne encephalitis (TBEV) viruses, which cause a number of serious human diseases throughout ...
H. Tian, X. Ji, Xiaoyun Yang, W. Xie, Kailin Yang, Cheng Chen, Chen Wu, H. Chi, Z. Mu, Zefang Wang, Haitao Yang   +10 more
semanticscholar   +1 more source

Structural basis of TFIIH activation for nucleotide excision repair. [PDF]

, 2019
Nucleotide excision repair (NER) is the major DNA repair pathway that removes UV-induced and bulky DNA lesions. There is currently no structure of NER intermediates, which form around the large multisubunit transcription factor IIH (TFIIH).
Chernev, A., Cramer, P., Dienemann, C., Kokic, G., Tegunov, D., Urlaub, H.   +5 more
core   +2 more sources

Crystal structure of the Bloom's syndrome helicase indicates a role for the HRDC domain in conformational changes

Nucleic Acids Research, 2015
Bloom's syndrome helicase (BLM) is a member of the RecQ family of DNA helicases, which play key roles in the maintenance of genome integrity in all organism groups. We describe crystal structures of the BLM helicase domain in complex with DNA and with an
J. Newman, P. Savitsky, C. Allerston, Anna H. Bizard, Özgün Özer, Kata Sarlós, Y. Liu, E. Pardon, J. Steyaert, I. Hickson, O. Gileadi   +10 more
semanticscholar   +1 more source