Results 131 to 140 of about 194,742 (384)

Transcription as a Threat to Genome Integrity [PDF]

, 2016
Genomes undergo different types of sporadic alterations, including DNA damage, point mutations, and genome rearrangements, that constitute the basis for evolution.
Aguilera López, Andrés, Gaillard, Hélène   +1 more
core   +1 more source

diPaRIS: Dynamic and Interpretable Protein‐RNA Interactions Prediction With U‐Shaped Network and Novel Structure Encoding

Advanced Science, EarlyView.
This study presents diPaRIS, a deep learning method designed to predict dynamic protein‐ RNA interactions with enhanced accuracy. By integrating RNA structures in vivo through a novel encoding scheme for nucleotide correlations, diPaRIS improves the interpretability of binding motifs and outperforms current state‐of‐the‐art methods across multiple ...
Lishen Zhang, Chengqian Lu, Xiaoqing Peng, Fei Guo, Hongdong Li, Jianxin Wang   +5 more
wiley   +1 more source

The Coronavirus helicase in replication

Virus Research
The coronavirus nonstructural protein (nsp) 13 encodes an RNA helicase (nsp13-HEL) with multiple enzymatic functions, including unwinding and nucleoside phosphatase (NTPase) activities. Attempts for enzymatic inactivation have defined the nsp13-HEL as a critical enzyme for viral replication and a high-priority target for antiviral development ...
Samantha L. Grimes, Mark R. Denison
openaire   +3 more sources

Opening of DNA double strands by helicases. Active versus passive opening [PDF]

, 2002
Helicase opening of double-stranded nucleic acids may be "active" (the helicase directly destabilizes the dsNA to promote opening) or "passive" (the helicase binds ssNA available due to a thermal fluctuation which opens part of the dsNA).
A. W. Hunter, B. Alberts, C. S. Peskin, Frank Jülicher, J. Howard, J. L. Kim, M. D. Betterton, M. R. Singleton, M. R. Singleton, M. S. Dillingham, M. S. Dillingham, P. H. von Hippel, S. Korolev, S. Korolev, S. S. Velankar   +14 more
core   +2 more sources

One‐Pot Isothermal Nucleic Acid Amplification Assisted CRISPR/Cas Detection Technology: Challenges, Strategies, and Perspectives

Advanced Science, EarlyView.
This review combines the current status of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat)‐based molecular diagnostic technology to expound the necessity and challenges faced in promoting the development of one‐pot detection. Besides, the focus is on a comprehensive summary and incisive analysis of emerging strategies for one‐pot ...
Hongyan Liu, Hui Yin, Leshan Xiu, Wenyu Wu, Qinqin Hu, Yingchen Xia, Brianna Garcia, Shamsuttiyeba Shifa, Hui Chen, Min Li, Kun Yin   +10 more
wiley   +1 more source

Joint Efforts of Replicative Helicase and SSB Ensure Inherent Replicative Tolerance of G‐Quadruplex

Advanced Science
G‐quadruplex (G4) is a four‐stranded noncanonical DNA structure that has long been recognized as a potential hindrance to DNA replication. However, how replisomes effectively deal with G4s to avoid replication failure is still obscure. Here, using single‐
Lijuan Guo, Yanling Bao, Yilin Zhao, Zhiyun Ren, Lulu Bi, Xia Zhang, Cong Liu, Xi‐Miao Hou, Michelle D. Wang, Bo Sun   +9 more
doaj   +1 more source

Protein cofactor competition regulates the action of a multifunctional RNA helicase in different pathways

RNA Biology, 2016
A rapidly increasing number of RNA helicases are implicated in several distinct cellular processes, however, the modes of regulation of multifunctional RNA helicases and their recruitment to different target complexes have remained unknown. Here, we show
A. Heininger, Philipp Hackert, Alexandra Z. Andreou, Kum-Loong Boon, Indira Memet, Mira Prior, A. Clancy, Bernhard Schmidt, H. Urlaub, E. Schleiff, K. Sloan, Markus Deckers, R. Lührmann, J. Enderlein, D. Klostermeier, P. Rehling, M. Bohnsack   +16 more
semanticscholar   +1 more source

Identification and functional analysis of novel phosphorylation sites in the RNA surveillance protein Upf1. [PDF]

, 2013
One third of inherited genetic diseases are caused by mRNAs harboring premature termination codons as a result of nonsense mutations. These aberrant mRNAs are degraded by the Nonsense-Mediated mRNA Decay (NMD) pathway.
Bracho, Dina P, Cajigas, Iván J, Correa, María E, Estrella, Luis A, González, Carlos I, González-Feliciano, José A, Lasalde, Clarivel, León, Alfredo J, Rivera, Andrea V, Rodríguez-Cruz, Eva N, Vega, Irving E, Wilkinson, Miles F   +11 more
core   +1 more source

K29‐Linked Ubiquitination of Transcription Regulators Controls Cell Proliferation in the Unfolded Protein Response

Advanced Science, EarlyView.
Under endoplasmic reticulum stress, cells activate the unfolded protein response (UPR). During the UPR, the cohesin complex at the promoter of cell proliferation‐related genes undergoes K29‐linked ubiquitination, recruiting the cohesin release factor WAPL to release cohesin, leading to transcriptional downregulation and inhibition of cell growth ...
Qiushuang Zhang, Xucong Teng, Yicong Dai, Yuncong Wu, Hongwei Hou, Jinghong Li   +5 more
wiley   +1 more source

Amidst multiple binding orientations on fork DNA, Saccharolobus MCM helicase proceeds N-first for unwinding

eLife, 2019
DNA replication requires that the duplex genomic DNA strands be separated; a function that is implemented by ring-shaped hexameric helicases in all Domains.
Himasha M Perera, Michael A Trakselis
doaj   +1 more source