Results 51 to 60 of about 194,742 (384)

A conserved Mcm4 motif is required for Mcm2-7 double-hexamer formation and origin DNA unwinding

eLife, 2019
Licensing of eukaryotic origins of replication requires DNA loading of two copies of the Mcm2-7 replicative helicase to form a head-to-head double-hexamer, ensuring activated helicases depart the origin bidirectionally.
Kanokwan Champasa, Caitlin Blank, Larry J Friedman, Jeff Gelles, Stephen P Bell   +4 more
doaj   +1 more source

Dynamics and Conformations of a Full-Length CRESS-DNA Replicase

Viruses, 2023
Circular Rep-encoding single-stranded DNA (CRESS-DNA) viruses encode for a Replicase (Rep) that is essential for viral replication. Rep is a helicase with three domains: an endonuclease, an oligomeric, and an ATPase domain (ED, OD, and AD).
Elvira Tarasova, Reza Khayat
doaj   +1 more source

Monomeric PcrA helicase processively unwinds plasmid lengths of DNA in the presence of the initiator protein RepD [PDF]

, 2013
The helicase PcrA unwinds DNA during asymmetric replication of plasmids, acting with an initiator protein, in our case RepD. Detailed kinetics of PcrA activity were measured using bulk solution and a single-molecule imaging technique to investigate the ...
Arbore, Arthur, Atkinson, Bird, Brune, Byrd, Byrd, Chao, Cheng, Christopher P. Toseland, Corrie, Dillingham, Dillingham, Dillingham, Dillingham, Dillingham, Fili, Fili, Gorbalenya, Greenstein, Gregory I. Mashanov, Iordanescu, Justin E. Molloy, Kunzelmann, Leatherbarrow, Lee, Levin, Liisa T. Chisty, Lohman, Lohman, Manosas, Mark S. Dillingham, Martin R. Webb, Mashanov, Natalia Fili, Niedziela-Majka, Noirot, Sellers, Singleton, Slatter, Soultanas, Soultanas, Soultanas, Subramanya, Thomas, Thomas, Toseland, Veaute, Velankar, Webb, Yamaguchi, Yang, Yarranton, Yeeles, Zhang   +54 more
core   +1 more source

Viral hijacking of a replicative helicase loader and its implications for helicase loading control and phage replication

eLife, 2016
Replisome assembly requires the loading of replicative hexameric helicases onto origins by AAA+ ATPases. How loader activity is appropriately controlled remains unclear.
Iris V Hood, James M Berger
doaj   +1 more source

Interaction and assembly of the DNA replication core proteins of Kaposi’s sarcoma-associated herpesvirus

Microbiology Spectrum, 2023
Kaposi’s sarcoma-associated herpesvirus (KSHV) encodes six highly conserved core replication proteins essential for the viral lytic DNA synthesis, including ORF6 (single-stranded DNA-binding protein), ORF9 (DNA polymerase), ORF40/41 (primase-associated ...
Lee-Wen Chen, Shie-Shan Wang, Li-Yu Chen, Hsiao-Yun Huang, Si-min He, Chien-Hui Hung, Chun-Liang Lin, Pey-Jium Chang   +7 more
doaj   +1 more source

Snf2 family ATPases and DExx box helicases:differences and unifying concepts from high-resolution crystal structures [PDF]

, 2006
Proteins with sequence similarity to the yeast Snf2 protein form a large family of ATPases that act to alter the structure of a diverse range of DNA–protein structures including chromatin. Snf2 family enzymes are related in sequence to DExx box helicases,
Aasland, Ahne, Akhtar, Andrew Flaus, Asturias, Auble, Auble, Auble, Bazett-Jones, Becker, Beerens, Bouazoune, Boyer, Boyer, Briggs, Caruthers, Corona, Cote, Dasgupta, Davis, Deaconescu, Dhalluin, Dumont, Durr, Eisen, Fan, Fitzgerald, Flaus, Flaus, Goldmark, Gorbalenya, Grune, Harald Dürr, Havas, Hishida, Ito, Jaskelioff, Kagalwala, Karl-Peter Hopfner, Kawaoka, Kim, Laurent, Laurent, Levin, Levin, Lia, Mahdi, Mannhaupt, Mizuguchi, Morillon, Myong, Peterson, Petukhova, Petukhova, Phelan, Pray-Grant, Quinn, Richmond, Ristic, Saha, Saha, Schild, Sengoku, Serebrov, Shen, Shinohara, Simic, Sims, Singleton, Singleton, Singleton, Smith, Soultanas, Sprouse, Stanley, Studitsky, Subramanya, Sukhodolets, Svejstrup, Swagemakers, Tai, Thoma, Tom Owen-Hughes, Tran, Tsukiyama, van den Boom, van Gool, Van Komen, Velankar, Wang, Whitehouse, Woudstra, Yu, Yu, Zhang, Zofall   +95 more
core   +3 more sources

Free-energy simulations reveal molecular mechanism for functional switch of a DNA helicase

eLife, 2018
Helicases play key roles in genome maintenance, yet it remains elusive how these enzymes change conformations and how transitions between different conformational states regulate nucleic acid reshaping.
Wen Ma, Kevin D Whitley, Yann R Chemla, Zaida Luthey-Schulten, Klaus Schulten   +4 more
doaj   +1 more source

RNA Unwinding by the Trf4/Air2/Mtr4 Polyadenylation (TRAMP) Complex [PDF]

, 2012
Many RNA-processing events in the cell nucleus involve the Trf4/Air2/Mtr4 polyadenylation (TRAMP) complex, which contains the poly(A) polymerase Trf4p, the Zn-knuckle protein Air2p, and the RNA helicase Mtr4p.
Anderson, James T., Jankowsky, Eckhard, Jia, Huijue, Wang, Xuying   +3 more
core   +2 more sources

Role of the Schizosaccharomyces pombe F-box DNA helicase in processing recombination intermediates. [PDF]

, 2005
In an effort to identify novel genes involved in recombination repair, we isolated fission yeast Schizosaccharomyces pombe mutants sensitive to methyl methanesulfonate (MMS) and a synthetic lethal with rad2.
Carr, Antony M, Furukawa, Fumiko, Iwasaki, Hiroshi, Morishita, Takashi, Sakaguchi, Chikako, Shinagawa, Hideo, Toda, Takashi   +6 more
core   +2 more sources

SUMOylated Senataxin functions in genome stability, RNA degradation, and stress granule disassembly, and is linked with inherited ataxia and motor neuron disease

Molecular Genetics & Genomic Medicine, 2021
Background Senataxin (SETX) is a DNA/RNA helicase critical for neuron survival. SETX mutations underlie two inherited neurodegenerative diseases: Ataxia with Oculomotor Apraxia type 2 (AOA2) and Amyotrophic Lateral Sclerosis type 4 (ALS4).
Craig L. Bennett, Albert R. La Spada
doaj   +1 more source