Results 51 to 60 of about 109,831 (197)

Novel Tetrazolium-Based Colorimetric Assay for Helicase nsp13 in SARS-CoV-2

BioChem
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is a human pathogenic virus that encodes for a helicase (SC2Hel) that is essential for viral replication. SC2Hel has the ability to unravel dsRNA or dsDNA in an NTP-dependent manner from the 5′
Triet M. Pham, Morgan G. Howard, Shane M. Carey, Lindsey R. Baker, Edward L. D’Antonio   +4 more
doaj   +1 more source

Structural basis of Zika virus helicase in recognizing its substrates

Protein & Cell, 2016
The recent explosive outbreak of Zika virus (ZIKV) infection has been reported in South and Central America and the Caribbean. Neonatal microcephaly associated with ZIKV infection has already caused a public health emergency of international concern.
Hongliang Tian, Xiaoyun Ji, Xiaoyun Yang, Zhongxin Zhang, Zuokun Lu, Kailin Yang, Cheng Chen, Qi Zhao, Heng Chi, Zhongyu Mu, Wei Xie, Zefang Wang, Huiqiang Lou, Haitao Yang, Zihe Rao   +14 more
doaj   +1 more source

The Role of miRNAs in Chicken Immune Regulation and Prospects for Disease‐Resistant Breeding

Animal Research and One Health, EarlyView.
A schematic workflow illustrating the screening of disease‐resistant miRNAs and the generation of miRNA‐based disease‐resistant chickens via PGC‐mediated germline genome editing. ABSTRACT MicroRNAs (miRNAs) are emerging as pivotal regulators of the immune system, playing a decisive role in shaping disease resistance in chicken.
Qiangzhou Wang, Weiran Wu, Qingqing Cai, Jiaxing Wang, Qi Gao, Muhammad Iftikhar, Yulin Bi, Hao Bai, Lihong Gu, Guobin Chang, Shihao Chen   +10 more
wiley   +1 more source

Extreme Kinetic Stability and RNase Resistance of Human Telomerase RNA G‐Quadruplexes Overcome by DHX36 Helicase

Advanced Science
RNA G‐quadruplexes (G4s) formed at the 5′‐end of the RNA component of human telomerase (hTR) are known to directly affect telomerase activity. However, the unfolding kinetics of hTR1–18 G4s at physiological K+ conditions have not been analyzed due to ...
Qun Luo, Yashuo Zhang, Yingxian Qian, Huiying Zhan, Wenqiang Wu, Huijuan You   +5 more
doaj   +1 more source

Dissociation of Rad51 Presynaptic Complexes and Heteroduplex DNA Joints by Tandem Assemblies of Srs2

Cell Reports, 2017
Srs2 is a superfamily 1 (SF1) helicase and antirecombinase that is required for genome integrity. However, the mechanisms that regulate Srs2 remain poorly understood.
Kyle Kaniecki, Luisina De Tullio, Bryan Gibb, Youngho Kwon, Patrick Sung, Eric C. Greene   +5 more
doaj   +1 more source

Duck Orbivirus and Egg Production: A Newly Identified Duck Orbivirus Is the Etiological Agent of Egg Production Decline in Chinese Breeder Ducks

Animal Research and One Health, EarlyView.
Field samples from breeder duck farms with egg‐production decline were subjected to viral detection, genome sequencing, and virus isolation. Experimental infection demonstrated that duck orbivirus (DORV) causes a significant and persistent reduction in egg production in breeder ducks.
Bing Li, Jianhua Wang, Xuezhi Cui, Xinxing Chu, You Wang, Dongmin Hao, Mingqing Xu, Haixia Chang, Huihui Li, Mingtian Mao, Mian Wu, Chengguang Lu, Jing Tang, Suyun Liang, Zhanbao Guo, Zhengkui Zhou, Youxiang Diao, Shuisheng Hou, Yi Tang   +18 more
wiley   +1 more source

Helicases at Work: The Importance of Nucleic Acids Unwinding Under Cold Stress

DNA
Separation of duplex strands of nucleic acids is a vital process in the nucleic acid metabolism and survival of all living organisms. Helicases are defined as enzymes that are intended to unwind the double-stranded nucleic acids.
Theetha L. Pavankumar, Navneet Rai, Pramod K. Pandey, Nishanth Vincent   +3 more
doaj   +1 more source

Recent Advances in Virus–Host Interactions, Antiviral Bioactive Compounds, and Breeding for Disease Resistance of Porcine Epidemic Diarrhea Virus

Animal Research and One Health, EarlyView.
Basic research on the PEDV infection cycle and virus–host interactions advances the development of anti‐PEDV drugs and disease‐resistant breeding and helps strengthen disease prevention and control while reducing economic losses in the swine industry.
Heyong Wu, Aosi Qi, Shuyu Zhong, Haozhen Du, Shimeng Guo, Hongsheng Ouyang, Hongming Yuan, Daxin Pang, Zicong Xie   +8 more
wiley   +1 more source

Increased plasma microbial tDR‐1 in at‐risk individuals is associated with decreased conversion to clinical rheumatoid arthritis and reduces an in vitro macrophage type 1 interferon response

Arthritis &Rheumatology, Accepted Article.
Background Microbial small RNAs (sRNAs) can regulate human genes. Higher plasma concentrations of microbial tRNA‐derived RNA‐1 (tDR‐1) were previously associated with lower rheumatoid arthritis (RA) disease activity. This study examined whether tDR‐1 concentrations differ in anti‐cyclic citrullinated peptide‐3 positive (CCP3+) at‐risk individuals (ARI)
Anastasiia Phothisane, Tulsi K Joishy, Carolina Ramirez‐Becerra, Qiong Wu, Jennifer Seifert, Marie L Feser, Jill M Norris, M Kristen Demoruelle, LauraKay Moss, Kevin D Deane, V Michael Holers, Michelle J Ormseth   +11 more
wiley   +1 more source

Amidst multiple binding orientations on fork DNA, Saccharolobus MCM helicase proceeds N-first for unwinding

eLife, 2019
DNA replication requires that the duplex genomic DNA strands be separated; a function that is implemented by ring-shaped hexameric helicases in all Domains.
Himasha M Perera, Michael A Trakselis
doaj   +1 more source