Results 61 to 70 of about 191,259 (287)

The mechanism of RNA duplex recognition and unwinding by DEAD-box helicase DDX3X

Nature Communications, 2019
DEAD-box helicases (DDXs) regulate RNA processing and metabolism by unwinding short double-stranded (ds) RNAs. Sharing a helicase core composed of two RecA-like domains (D1D2), DDXs function in an ATP-dependent, non-processive manner.
He Song, X. Ji
semanticscholar   +1 more source

Monomeric PcrA helicase processively unwinds plasmid lengths of DNA in the presence of the initiator protein RepD [PDF]

, 2013
The helicase PcrA unwinds DNA during asymmetric replication of plasmids, acting with an initiator protein, in our case RepD. Detailed kinetics of PcrA activity were measured using bulk solution and a single-molecule imaging technique to investigate the ...
Arbore, Arthur, Atkinson, Bird, Brune, Byrd, Byrd, Chao, Cheng, Christopher P. Toseland, Corrie, Dillingham, Dillingham, Dillingham, Dillingham, Dillingham, Fili, Fili, Gorbalenya, Greenstein, Gregory I. Mashanov, Iordanescu, Justin E. Molloy, Kunzelmann, Leatherbarrow, Lee, Levin, Liisa T. Chisty, Lohman, Lohman, Manosas, Mark S. Dillingham, Martin R. Webb, Mashanov, Natalia Fili, Niedziela-Majka, Noirot, Sellers, Singleton, Slatter, Soultanas, Soultanas, Soultanas, Subramanya, Thomas, Thomas, Toseland, Veaute, Velankar, Webb, Yamaguchi, Yang, Yarranton, Yeeles, Zhang   +54 more
core   +1 more source

Terminating the replication helicase

Nature Cell Biology, 2017
A feature of the cell cycle is that the events of one cycle must be reset before the next one begins. A study now shows that the replication machinery is removed from fully replicated DNA by a conserved ubiquitin- and CDC48 (also known as p97)-dependent pathway. This explains how eukaryotic chromosomes are returned to the unreplicated state.
Gaggioli, Vincent, Zegerman, Philip
openaire   +4 more sources

Structure Prediction and Analysis of Hepatitis E Virus Non-Structural Proteins from the Replication and Transcription Machinery by AlphaFold2

Viruses, 2022
Hepatitis E virus (HEV) is a major cause of acute viral hepatitis in humans globally. Considered for a long while a public health issue only in developing countries, the HEV infection is now a global public health concern.
Adeline Goulet, Christian Cambillau, Alain Roussel, Isabelle Imbert   +3 more
doaj   +1 more source

Velocity and processivity of helicase unwinding of double-stranded nucleic acids

, 2005
Helicases are molecular motors which unwind double-stranded nucleic acids (dsNA) in cells. Many helicases move with directional bias on single-stranded (ss) nucleic acids, and couple their directional translocation to strand separation.
Bhattacharjee S M, Doering C, F Jülicher, Klein G A, Korolev S, Lohman T M, M D Betterton, Perkins T T, Peskin C S, Stukalin E B   +9 more
core   +1 more source

Interfacial instability and DNA fork reversal by repair proteins [PDF]

, 2009
A repair protein like RecG moves the stalled replication fork in the direction from the zipped to the unzipped state of DNA. It is proposed here that a softening of the zipped-unzipped interface at the fork results in the front propagating towards the ...
Bhattacharjee, Somendra M.
core   +2 more sources

SIRT7 and the DEAD-box helicase DDX21 cooperate to resolve genomic R loops and safeguard genome stability

Genes & Development, 2017
Here, Song et al. show that the DEAD-box RNA helicase DDX21 efficiently unwinds RNA/DNA hybrids (R loops) in vivo and in vitro and prevents DNA damage. Their findings reveal a novel mechanism by which accumulation of R loops by DDX21 depletion leads to ...
Chenlin Song, A. Hotz-Wagenblatt, R. Voit, I. Grummt   +3 more
semanticscholar   +1 more source

Structure of a translocation signal domain mediating conjugative transfer by Type IV secretion systems [PDF]

, 2013
Relaxases are proteins responsible for the transfer of plasmid and chromosomal DNA from one bacterium to another during conjugation. They covalently react with a specific phosphodiester bond within DNA origin of transfer sequences, forming a nucleo ...
Gruber, C.J., Ilangovan, Aravindan, Lang, S., Redzej, Adam, Topf, Maya, Waksman, Gabriel, Zangger, K., Zechner, E.L.   +7 more
core   +1 more source

Structural insights into RNA recognition by the Chikungunya virus nsP2 helicase

Proceedings of the National Academy of Sciences of the United States of America, 2019
Significance The multifunctional helicase-protease protein nsP2 of Chikungunya virus (CHIKV) plays vital roles in viral RNA replication and virus–host interactions.
Y. Law, Age Utt, Yaw Bia Tan, Jie Zheng, Sainan Wang, M. Chen, P. Griffin, A. Merits, D. Luo   +8 more
semanticscholar   +1 more source

RNA helicases in splicing [PDF]

RNA Biology, 2013
In eukaryotic cells, introns are spliced from pre-mRNAs by the spliceosome. Both the composition and the structure of the spliceosome are highly dynamic, and eight DExD/H RNA helicases play essential roles in controlling conformational rearrangements. There is evidence that the various helicases are functionally and physically connected with each other
Jean D. Beggs, Olivier Cordin
openaire   +3 more sources