Results 11 to 20 of about 2,734 (113)

In vitro stimulation of antibody formation by peritoneal cells: I. Plaque technique of high sensitivity enabling access to the cells

, 1970
An improved method for the short-term culture of mouse peritoneal cells in a medium containing carboxymethylcellulose (CMC), sheep erythrocytes (SRBC), and guinea pig complement is described.
Lewis, H, Nossal, GJV, Bussard, AE, Mazie, JC   +3 more
core   +1 more source

Adaptation of the reverse hemolytic plaque assay to electron microscopy: a study of the individual secretory activity in prolactin cell subpopulations.

, 1994
We used the reverse hemolytic plaque assay (RHPA), a method for detecting secretion by single cells, to demonstrate functional heterogeneity among prolactin (PRL) cells of rat anterior pituitary at the light microscopic (LM) level. We attempted to adapt
R Picart, E Vila-Porcile, C Tougard
core   +1 more source

Comparison of lactotrope subtypes of neonatal and adult male rats: plaque assays and patch-clamp studies

, 1993
We examined the differences in lactotrope number and function between pituitary cultures from neonatal (10-day-old) and adult male rats. Basal hormone release was measured with the reverse hemolytic plaque assay.
J. Horta, R. Felix, G. Cota
core   +1 more source

Identification of individual renocortical cells that secrete renin

, 1990
Successful application of the reverse hemolytic plaque assay was developed to identify individual renocortical cells that secrete renin directly. The plaque assay was validated by a number of established criteria. Using this technique, we demonstrate an
M. K. Hunt, T. Inagami, K. M. Geary, M. J. Peach, D. A. Leong, S. P. Ramos, M. S. Forbes, R. M. Carey   +7 more
core   +1 more source

Membrane currents in identified lactotrophs of rat anterior pituitary

, 1986
Qualitative features of the primary inward and outward current components of identified lactotrophs of the rat anterior pituitary were examined. Identification of lactotrophs in heterogeneous dissociated anterior pituitary cultures was accomplished by ...
CJ Lingle, S Sombati, ME Freeman
core   +1 more source

Multi‐Enzyme Mimetic Molybdenum Nitride Nanozymes Reshape Subgingival Microenvironment for Synergistic Periodontitis Therapy via ROS Regulation and Microbiome Remodeling

Advanced Science, EarlyView.
ABSTRACT Periodontitis, a chronic inflammatory disease initiated and sustained by plaque microorganisms and host immune response, remains an intractable oral disease and a leading cause of tooth loss worldwide. Traditional mechanical debridement and adjunctive antibiotic or antiseptic therapy often shows limited efficacy due to the complex anatomical ...
Weiyu Zhang, Zhongwei Yang, Yunfan Zhang, Longwei Wang, Xiaoyu Zhang, Jiahui Mao, Yizhi Dai, Yifan Yuan, Mingge Wang, Xin Yang, Xin Yu, Jing Liu, Chunying Chen   +12 more
wiley   +1 more source

Brain and Liver Dual‐Targeting Oridonin Nanoparticles to Enhance Aβ Clearance for Alzheimer's Disease Therapy

Advanced Science, EarlyView.
We developed a nanoparticle named OAF, which simultaneously targeted to both the brain and liver via the transferrin receptor 1 (TfR1) receptor, promoting lipoprotein receptor‐related protein 1 (LRP1) expression to enhance amyloid‐beta (Aβ) clearance. In AD mice model, OAF significantly reduced Aβ deposition and cognitive impairment, while a mitigating
Wenshuai Gong, Wenting Hui, Sai Qiao, Qifeng Ji, Miao Liu, Bangle Zhang, Daozhou Liu, Yumei Wu, Siyuan Zhou   +8 more
wiley   +1 more source

Pathological Mechanism‐Inspired Biomimetic Nano‐Senotherapy for Reversing Experimental Atherosclerosis in ApoE−/− Mice

Advanced Science, EarlyView.
The biomimetic self‐assembly nanomedicine reversing atherosclerosis via senotherapy strategy. ABSTRACT The greatest challenge in atherosclerosis (AS) management lies in achieving lesion reversal, not merely slowing progression. Senescent cell accumulation—driven by continuous generation and apoptotic resistance—perpetuates plaque pathology and ...
Yuhan Tian, Yanrui Yang, Qiuyu Li, Ying Hu, Qin Hu, Shasha Ran, Siyu Zhou, Xiujuan Yang, Shanshan Li, Qixiong Zhang   +9 more
wiley   +1 more source

A background-free assay for cells forming antibody to glucose oxidase coupled with an enzyme-linked immunosorbent assay (ELISA) to quantitate antibody secretion.

, 1989
We describe an immunocytochemical assay for cells forming antibody to glucose oxidase (GO). The method is specific in that only cells containing intracytoplasmic antibody capable of binding the immunogen (GO binding cells; GOBC) are stained.
W G Miller, R C Hard, G Romagnoli
core   +1 more source

Research progress on biomarkers of traumatic brain injury

Animal Models and Experimental Medicine, EarlyView.
Traumatic brain injury: From primary insult to secondary neuroinflammation and degeneration. Abstract Traumatic brain injury (TBI) is a common disorder of the nervous system and has become a leading cause of death and disability worldwide, imposing a substantial burden on patients and their social circles. Its main symptoms include dyskinesia, language
Xuting Shen, Si Cheng, Shuyi Chen, Mengyue Wang, Tangying Li, Weicheng Xu, Yifan Zhang, Ping Yuan, Lei Shi   +8 more
wiley   +1 more source