Results 1 to 10 of about 348,405 (166)

A nanowell platform to identify, sort and expand high antibody-producing cells [PDF]

Scientific Reports
Increased use of therapeutic monoclonal antibodies and the relatively high manufacturing costs fuel the need for more efficient production methods. Here we introduce a novel, fast, robust, and safe isolation platform for screening and isolating antibody ...
Fikri Abali, Richard Schasfoort, Sanne Nijland, Jelle Wittenberns, Arjan. G. J. Tibbe, Marcel den Hartog, Louis Boon, Leon W. M. M. Terstappen   +7 more
doaj   +2 more sources

FUNCTIONAL RIBOSOMES IN ANTIBODY-PRODUCING CELLS [PDF]

Proceedings of the National Academy of Sciences of the United States of America, 1964
Kurt H Stenzel, David D Thompson, Phillips William D   +2 more
exaly   +3 more sources

FK506-Binding Protein 11 Is a Novel Plasma Cell-Specific Antibody Folding Catalyst with Increased Expression in Idiopathic Pulmonary Fibrosis

Cells, 2022
Antibodies are central effectors of the adaptive immune response, widespread used therapeutics, but also potentially disease-causing biomolecules. Antibody folding catalysts in the plasma cell are incompletely defined. Idiopathic pulmonary fibrosis (IPF)
Stefan Preisendörfer, Yoshihiro Ishikawa, Elisabeth Hennen, Stephan Winklmeier, Jonas C. Schupp, Larissa Knüppel, Isis E. Fernandez, Leonhard Binzenhöfer, Andrew Flatley, Brenda M. Juan-Guardela, Clemens Ruppert, Andreas Guenther, Marion Frankenberger, Rudolf A. Hatz, Nikolaus Kneidinger, Jürgen Behr, Regina Feederle, Aloys Schepers, Anne Hilgendorff, Naftali Kaminski, Edgar Meinl, Hans Peter Bächinger, Oliver Eickelberg, Claudia A. Staab-Weijnitz   +23 more
doaj   +1 more source

The immunosenescence-related factor DOCK11 is involved in secondary immune responses of B cells

Immunity & Ageing, 2022
Background Memory B cells are an antigen-experienced B-cell population with the ability to rapidly differentiate into antibody-producing cells by recall responses.
Yuma Sugiyama, Mitsuhiro Fujiwara, Akihiko Sakamoto, Hiromichi Tsushima, Akihiko Nishikimi, Mitsuo Maruyama   +5 more
doaj   +1 more source

Detection of circulating B cells producing anti-GPIb autoantibodies in patients with immune thrombocytopenia. [PDF]

PLoS ONE, 2014
BACKGROUND: We previously reported that an enzyme-linked immunospot (ELISPOT) assay for detecting anti-GPIIb/IIIa antibody-secreting B cells is a sensitive method for identifying patients with immune thrombocytopenia (ITP).
Masataka Kuwana, Yuka Okazaki, Yasuo Ikeda   +2 more
doaj   +1 more source

STUDIES ON ANTIBODY-PRODUCING CELLS [PDF]

The Journal of Experimental Medicine, 1970
Efferent lymph of the popliteal lymph nodes of rabbits was collected 4 days after a single footpad injection of SRBC. Thin-layer agar plating was done to isolate plaque-forming cells of the lymph for electron microscope examination, and the numbers of plaque-forming cells (PFC) in cells from the lymph and lymph nodes were determined.
Fred G. Gudat, T. N. Harris, Susanna Harris, Klaus Hummeler   +3 more
openaire   +4 more sources

Introduction of direct-acting antiviral agents alters frequencies of anti-GPIIb/IIIa antibody-producing B cells in chronic hepatitis C patients with thrombocytopenia

Platelets, 2023
The pathogenesis of thrombocytopenia in chronic hepatitis C (CHC) conceivably involves autoimmunity; however, the dynamics of autoantibodies and other autoimmune mechanisms remain unclear.
Takashi Satoh, Haruki Uojima, Naohisa Wada, Hayato Takiguchi, Mei Kaneko, Marina Nakamura, Natsuki Gonda, Michika Homma, Hisashi Hidaka, Chika Kusano, Ryouichi Horie   +10 more
doaj   +1 more source

Intrathymic differentiation of natural antibody-producing plasma cells in human neonates

Nature Communications, 2021
The thymus is known as the organ of T lymphocyte development. Here authors show that terminal B cell differentiation also takes place in the thymus of human neonates, leading to antibody production against commensal and pathogenic bacteria.
Hector Cordero, Rodney G. King, Pranay Dogra, Chloe Dufeu, Sarah B. See, Alexander M. Chong, Anne-Catrin Uhlemann, Siu-Hong Ho, David M. Kalfa, Emile A. Bacha, John F. Kearney, Emmanuel Zorn   +11 more
doaj   +1 more source

High-throughput single-cell antibody secretion quantification and enrichment using droplet microfluidics-based FRET assay

iScience, 2022
Summary: High-throughput screening and enrichment of antibody-producing cells have many important applications. Herein, we present a droplet microfluidic approach for high-throughput screening and sorting of antibody-secreting cells using a Förster ...
Justina Rutkauskaite, Simon Berger, Stavros Stavrakis, Oliver Dressler, John Heyman, Xavier Casadevall i Solvas, Andrew deMello, Linas Mazutis   +7 more
doaj   +1 more source

Immortalisation of Human Antibody Producing Cells

Autoimmunity, 1992
(1992). Immortalisation of Human Antibody Producing Cells. Autoimmunity: Vol. 13, No. 3, pp. 249-258.
J K, Kalsi, D A, Isenberg
openaire   +2 more sources