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Epizootic hemorrhagic disease virus serotype 6 experimentation on adult cattle
Research in Veterinary Science, 2013Epizootic hemorrhagic disease virus (EHDV), an arthropod-borne orbivirus (family Reoviridae), is an emerging pathogen of wild and domestic ruminants closely related to bluetongue virus (BTV). EHDV serotype 6 (EHDV6) has recently caused outbreaks close to Europe in Turkey and Morocco and a recent experimental study performed on calves inoculated with ...
Bréard, Emmanuel +13 more
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Epizootic hemorrhagic disease virus infection of type I interferon receptor deficient mice
Veterinary Microbiology, 2012Type I interferon receptor deficient (IFNAR(-/-)) mice were infected with an Israeli isolate of serotype 7 of epizootic hemorrhagic disease virus (EHDV; Orbivirus, Reoviridae). Two out of two mice that received 5×10(5) 50% tissue culture infectious doses (TCID(50)) by intraperitoneal injection died or were euthanized in a moribund state on day 5 after ...
Michael, Eschbaumer +3 more
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Hemagglutination of epizootic hemorrhagic disease virus
Archives of Virology, 1981Hemagglutination of epizootic hemorrhagic disease virus (EHDV) with a variety of erythrocyte species at 4 degrees C, room temperature and 37 degrees C was dependent on the NaCl molarity and the pH of the diluent. The hemagglutination inhibition test was used to identify EHDV serotypes.
S, Tokuhisa +6 more
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Purification and stability of epizootic hemorrhagic disease virus
Journal of Virological Methods, 1982Purification of epizootic hemorrhagic disease virus (EHDV) from sonicated cell culture supernatant was effected using polyethylene glycol (PEG) precipitation and two CsCl density gradient centrifugations. During purification procedures the pH was maintained at values close to pH 8.0 as pH stability for EHDV was maximal between pH 7.2 and pH 9.0. During
R, Lewanczuk, T, Yamamoto
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Experimental infection of calves with epizootic hemorrhagic disease virus
American Journal of Veterinary Research, 1999Abstract Objective To determine whether experimental inoculation with a field strain of epizootic hemorrhagic disease virus serotype-2 (EHDV-2) suspected of causing clinical disease in naturally infected cattle would cause clinical disease in calves. Animals 8 calves.
M J, Abdy +2 more
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Epizootic Hemorrhagic Disease Virus in Montana: Isolation and Serologic Survey
American Journal of Veterinary Research, 1981SUMMARY Epizootic hemorrhagic disease virus (ehdv) was isolated in Vero cell culture from the spleen and whole blood of a white-tailed deer (Odocoileus virginianus). A 10% spleen suspension caused acute hemorrhagic disease (hd) when inoculated into an experimental white-tailed deer and resulted in the recovery of ehdv from the blood of the experimental
T J, Feldner, M H, Smith
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Epizootic hemorrhagic disease virus of deer: an electron microscopic study
Canadian Journal of Microbiology, 1970A mouse brain adapted strain of epizootic hemorrhagic disease (EHD) virus of deer was studied by ultrathin section and negative contrast electron microscopy. In sections of infected BHK-21 cells and neurons of newborn mouse brains, aggregates of virus particles, some appearing to be lacking their outer coats, were observed within membrane-bound ...
K S, Tsai, L, Karstad
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Clinical disease associated with epizootic hemorrhagic disease virus in cattle in Illinois
Journal of the American Veterinary Medical Association, 2015Abstract Objective—To describe the clinical manifestation of epizootic hemorrhagic disease virus (EHDV) in cattle during an outbreak in northwestern Illinois in the fall of 2013. Design—Outbreak survey. Sample—18 EHDV-affected cattle herds. Procedures—Herds with confirmed EHDV-infected cattle were selected for the survey on the basis of having a ...
Edgar F, Garrett +5 more
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