Results 171 to 180 of about 2,865 (201)
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The Bovine Practitioner, 2018
Epizootic hemorrhagic disease virus is an orbivirus transmitted by Culicoides spp. In North America, it is recognized as a major cause of disease affecting white-tailed deer, but it can impact other wildlife species as well as domestic cattle. Although minimal clinical disease had been noted in Texas, periodic outbreaks in other states caused Texas ...
Hairgrove, Thomas B. +4 more
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Epizootic hemorrhagic disease virus is an orbivirus transmitted by Culicoides spp. In North America, it is recognized as a major cause of disease affecting white-tailed deer, but it can impact other wildlife species as well as domestic cattle. Although minimal clinical disease had been noted in Texas, periodic outbreaks in other states caused Texas ...
Hairgrove, Thomas B. +4 more
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Vaccines, 2020 Epizootic hemorrhagic disease virus (EHDV) is an arthropod-transmitted RNA virus and the causative agent of epizootic hemorrhagic disease (EHD) in wild and domestic ruminants.
Sun-Young Sunwoo +2 more
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Molecular and genetic comparisons of two serotypes of epizootic hemorrhagic disease of deer virus
American Journal of Veterinary Research, 1986SUMMARY The virus-specific double-stranded genome RNA of 2 serotypes of epizootic hemorrhagic disease of deer virus (ehdv) was evaluated by use of coelectrophoresis in polyacrylamide and agarose gel systems. The molecular weights of virion RNA segments were 0.32 to 2.57 × 106 for ehdv-1 and 0.33 to 2.54 × 106 for ehdv-2. Seven of 10 double-stranded RNA
J, Kusari, P, Roy
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TRANSMISSION OF TWO STRAINS OF EPIZOOTIC HEMORRHAGIC DISEASE VIRUS IN DEER BY Culicoides variipennis
Journal of Wildlife Diseases, 1977Two strains of epizootic hemorrhagic disease virus (EHDV), New Jersey (NJ) and Kentucky (KY), of deer were biologically transmitted between white-tailed deer, Odocoileus virginianus, by Culicoides variipennis. The KY strain, isolated from C. variipennis collected during an epizootic in deer, was identified as EHDV by serological tests.
N. M. FOSTER +4 more
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American Journal of Veterinary Research, 1988
SUMMARY Epizootic hemorrhagic disease virus was isolated from cattle and sheep in northeastern Colorado during July and August 1984. The isolates were identified as serotype 2 by plaque-inhibition serotyping, genome electropherotyping, and protein analysis.
L H, Thompson +2 more
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SUMMARY Epizootic hemorrhagic disease virus was isolated from cattle and sheep in northeastern Colorado during July and August 1984. The isolates were identified as serotype 2 by plaque-inhibition serotyping, genome electropherotyping, and protein analysis.
L H, Thompson +2 more
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Canadian Journal of Microbiology, 1970
A plaque assay system for the virus of epizootic hemorrhagic disease of deer in the WI-2 subline of BHK-21 cells is described. The system uses infection of the cells in suspension before growth as a monolayer under supplemented Leibovitz L-15 medium incorporating gum tragacanth. Crude cell lysates were initially used as a source of virus for the assay
N G, Willis +3 more
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A plaque assay system for the virus of epizootic hemorrhagic disease of deer in the WI-2 subline of BHK-21 cells is described. The system uses infection of the cells in suspension before growth as a monolayer under supplemented Leibovitz L-15 medium incorporating gum tragacanth. Crude cell lysates were initially used as a source of virus for the assay
N G, Willis +3 more
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Epizootic Hemorrhagic Disease Virus Titration
The titration of viruses onto susceptible cell lines is an important virological technique used to quantify infectious viral titers. It forms an integral component of epizootic hemorrhagic disease virus (EHDV) research, including estimating infectivity, calculating multiplicity of infection, and confirming virus propagation in cell culture.openaire +2 more sources
Journal of Medical Entomology, 1991
Nucleic acid hybridization was used to detect epizootic hemorrhagic disease (EHD) virus serotype 1 and serotype 2 in Culicoides variipennis (Coquillett). Adult females were inoculated intrathoracically with virus, then were assayed daily for the presence of viral RNA for 2 wk, at which time maximum virus replication is likely to occur. Viral RNA of EHD
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Nucleic acid hybridization was used to detect epizootic hemorrhagic disease (EHD) virus serotype 1 and serotype 2 in Culicoides variipennis (Coquillett). Adult females were inoculated intrathoracically with virus, then were assayed daily for the presence of viral RNA for 2 wk, at which time maximum virus replication is likely to occur. Viral RNA of EHD
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Infection of Culicoides Biting Midges with Epizootic Hemorrhagic Disease Virus
Epizootic hemorrhagic disease virus (EHDV) is transmitted by Culicoides biting midges. Studies aiming to predict the likely spread of EHDV require an understanding of the viral infection and replication kinetics within these insects, including the proportion of the insect population that are able to support virus transmission. Here, we describe methodsChristopher, Sanders, Kerry, Newbrook
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Molecular Serotyping of Epizootic Hemorrhagic Disease Virus
Molecular methods are routinely used for the differential diagnosis and genetic characterization of viral disease of livestock. Real-time, quantitative PCR (qPCR) allows RNA/DNA sequence detection and quantification and is considered the gold standard diagnostic method for most viruses.openaire +2 more sources