Results 181 to 190 of about 5,585 (211)

EXPERIMENTAL INFECTION IN NORTH AMERICAN ELK WITH EPIZOOTIC HEMORRHAGIC DISEASE VIRUS

Journal of Wildlife Diseases, 1973
Two elk (Cervus canadensis) were inoculated intravenously with epizootic hemorrhagic disease virus. No overt signs of disease were observed in the elk although one animal had a slight febrile response. A cell associated viremia was detected in both elk, which persisted in one animal for 30 days post-inoculation.
G L, Hoff, D O, Trainer
openaire   +2 more sources

Plaque Neutralization of Bluetongue Virus and Epizootic Hemorrhagic Disease Virus in BHK21 Cells

American Journal of Veterinary Research, 1976
SUMMARY Plaque assay and plaque neutralization of bluetongue virus and epizootic hemorrhagic disease virus were studied in baby hamster kidney (bhk21) cells grown under an overlay containing gum tragacanth. Tests were done in plastic panels, each with 24 wells, and variables were established for achieving reproducible results.
M M, Jochim, S C, Jones
openaire   +2 more sources

The association of epizootic hemorrhagic disease virus with the cytoskeleton

Proceedings, annual meeting, Electron Microscopy Society of America, 1990
The cells of eukaryotes are characterized by a filamentous network referred to as the cytoskeleton. It is believed that most animal viruses use the cytoplasmic or nuclear skeletal matrix during at least part of their replication cycle.Transmission electron microscopic studies of thin sections of cells infected with epizootic hemorrhagic disease virus ...
R.A. Nunamaker, J.G. Wigington, C.E. Nunamaker   +2 more
openaire   +1 more source

Epizootic Hemorrhagic Disease Virus Titration

The titration of viruses onto susceptible cell lines is an important virological technique used to quantify infectious viral titers. It forms an integral component of epizootic hemorrhagic disease virus (EHDV) research, including estimating infectivity, calculating multiplicity of infection, and confirming virus propagation in cell culture.
openaire   +2 more sources

Detection of Epizootic Hemorrhagic Disease Virus in Culicoides variipennis (Diptera: Ceratopogonidae)

Journal of Medical Entomology, 1991
Nucleic acid hybridization was used to detect epizootic hemorrhagic disease (EHD) virus serotype 1 and serotype 2 in Culicoides variipennis (Coquillett). Adult females were inoculated intrathoracically with virus, then were assayed daily for the presence of viral RNA for 2 wk, at which time maximum virus replication is likely to occur. Viral RNA of EHD
openaire   +2 more sources

Review of epizootic hemorrhagic disease in cattle and a study defining seroprevalence of epizootic hemorrhagic disease virus serotype 2 in Texas cattle

The Bovine Practitioner, 2018
Epizootic hemorrhagic disease virus is an orbivirus transmitted by Culicoides spp. In North America, it is recognized as a major cause of disease affecting white-tailed deer, but it can impact other wildlife species as well as domestic cattle. Although minimal clinical disease had been noted in Texas, periodic outbreaks in other states caused Texas ...
Hairgrove, Thomas B., Rodgers, Sandy M., Cook, Walter M., Budke, Christine M., Smith, William B.   +4 more
openaire   +1 more source

Infection of Culicoides Biting Midges with Epizootic Hemorrhagic Disease Virus

Epizootic hemorrhagic disease virus (EHDV) is transmitted by Culicoides biting midges. Studies aiming to predict the likely spread of EHDV require an understanding of the viral infection and replication kinetics within these insects, including the proportion of the insect population that are able to support virus transmission. Here, we describe methods
Christopher, Sanders, Kerry, Newbrook
openaire   +2 more sources

Molecular Serotyping of Epizootic Hemorrhagic Disease Virus

Molecular methods are routinely used for the differential diagnosis and genetic characterization of viral disease of livestock. Real-time, quantitative PCR (qPCR) allows RNA/DNA sequence detection and quantification and is considered the gold standard diagnostic method for most viruses.
openaire   +2 more sources

Whole Genome Sequencing of Epizootic Hemorrhagic Disease Virus

Next-generation sequencing (NGS) technologies are continuously being developed and are becoming a more cost-effective tool for the characterization of viral genomes. Whole genome sequencing of segmented viruses, such as epizootic hemorrhagic disease virus (EHDV), provides insights into the molecular epidemiology as well as such viral evolutionary ...
openaire   +2 more sources

TRANSMISSION OF TWO STRAINS OF EPIZOOTIC HEMORRHAGIC DISEASE VIRUS IN DEER BY Culicoides variipennis

Journal of Wildlife Diseases, 1977
Two strains of epizootic hemorrhagic disease virus (EHDV), New Jersey (NJ) and Kentucky (KY), of deer were biologically transmitted between white-tailed deer, Odocoileus virginianus, by Culicoides variipennis. The KY strain, isolated from C. variipennis collected during an epizootic in deer, was identified as EHDV by serological tests.
N. M. FOSTER, R. D. BRECKON, A. J. LUEDKE, R. H. JONES, H. E. METCALF   +4 more
openaire   +2 more sources