Integrated one‐pot CRISPR‐Cas detection: Addressing strategies and future prospects
Integrated one‐pot CRISPR‐Cas detection significantly simplifies workflows, reduces cross‐contamination risks, and shortens turnaround times, making it highly suitable for point‐of‐care testing (POCT) and resource‐limited settings. Key challenges include system compatibility issues between Cas proteins and isothermal amplification, limited multiplexing
Jian Zhou +6 more
wiley +1 more source
Frequency of Hepatitis B and Hepatitis C Infections Among Patients With Chronic Kidney Disease: A Retrospective Data-Based Study From Lahore, Pakistan. [PDF]
Sami MT +5 more
europepmc +1 more source
Enhanced CRISPR‐cas systems for genome editing and molecular diagnostics
This review systematically summarizes the major optimization strategies for CRISPR‐Cas systems, focusing on the engineering of Cas proteins, guide RNAs, reporter probes, and the incorporation of chemical additives. The aim is to enhance editing precision and diagnostic capability, thereby expanding the CRISPR system's potential for broader biomedical ...
Sheng Li +7 more
wiley +1 more source
Performance evaluation of methods for detecting enteric viruses in meat products. [PDF]
Zanchetta L +7 more
europepmc +1 more source
Schematic overview of the human microbiome and major microbiota‐derived metabolites across body sites, highlighting the gut–brain, gut–heart, and gut–kidney axes in host physiology and disease. ABSTRACT Background The human microbiome is a dynamic and diverse community of microorganisms that affects susceptibility to illness and promotes wellness ...
Awadh Alanazi
wiley +1 more source
The Role of Human Viral Entry Receptor Mouse Models in Advancing Antiviral Antibodies and Vaccines. [PDF]
Zuo N, Zheng X, Ishaq R, Ren D, Hu A.
europepmc +1 more source
Student Anxiety and Stress Related to Needlestick/Sharps Injuries in Dental Clinics
ABSTRACT Purpose/Objective This study explored how the threat of needlestick/sharps injuries affects stress and anxiety among dental, dental hygiene, and dental assisting students in accredited US programs. It also aimed to identify contributing factors and assess potential educational interventions.
Lindsey C. Billing, Marisa A. Cornewell
wiley +1 more source
Outbreak of FAdV-4 infection in blue-breasted quail (Excalfactoria chinensis) reveals novel cross-species transmission of virus in domestic poultry and ornamental pet birds. [PDF]
Teng M +6 more
europepmc +1 more source
Abstract Background Metabolic dysfunction‐associated steatohepatitis (MASH) is associated with altered intestinal permeability, allowing microbial products to translocate to the liver via the enterohepatic circulation and contributes to disease progression. The aim of our study was to evaluate the association between probiotic supplementation and serum
Bruna Concheski de Moura +10 more
wiley +1 more source

