Results 101 to 110 of about 196,489 (314)

High‐Throughput Engineering and Modification of Non‐Ribosomal Peptide Synthetases Based on Golden Gate Assembly

Angewandte Chemie, EarlyView.
A Golden Gate Assembly (GGA)‐based method was developed for the efficient assembly of natural and engineered non‐ribosomal peptide synthetases (NRPS). This method has enabled the creation of NRPS libraries to generate novel peptides in high‐throughput as well as the targeted derivatisation of natural products (NPs).
Adrian Podolski, Timon A. Lindeboom, Leonard Präve, Maryam Dehghan, Hannah A. Minas, Janik Kranz, Daniel Schindler, Helge B. Bode   +7 more
wiley   +2 more sources

The use of heterologous antilymphoid agents in canine renal and liver homotransplantation and in human renal homotransplantation [PDF]

, 1967
TE Starzl, Thomas L. Marchioro, K. A. Porter, Y. Iwasaki, G J Cirelli   +4 more
openalex   +1 more source

Parabiosis, Assembloids, Organoids (PAO)

Advanced Science, EarlyView.
This review evaluates parabiosis, organoids, and assembloids as complementary disease models spanning systemic, organ, and multi‐organ levels. It highlights their construction strategies, applications, and current limitations, while emphasizing their integration with frontier technologies such as artificial intelligence, organ‐on‐a‐chip, CRISPR, and ...
Yang Hong, Lavonda Li, Lijie Yan, Long Bai, Jiacan Su, Xingcai Zhang   +5 more
wiley   +1 more source

Studies of α′,β′‐Epoxyketone Synthesis by Small‐Molecule Flavins and Flavoenzymes

Angewandte Chemie, EarlyView.
A combined synthetic and biochemical approach links the flavoenzyme EpxF to three key steps in epoxomicin biosynthesis. Crystallography, mutagenesis, and 13C‐labeling complete the mechanistic picture of α′,β′‐epoxyketone formation and illustrate the potential of flavin‐mediated transformations.
Alexandra Walter, Wolfgang Kuttenlochner, Wolfgang Eisenreich, Chengyang Yao, Michael Groll, Golo Storch   +5 more
wiley   +2 more sources

An Inflammation‐Targeting Engineered Probiotic Escherichia coli Nissle 1917 with High Anti‐TNF‐α Nanobody Secretion Efficacy Alleviates Ulcerative Colitis

Advanced Science, EarlyView.
Ulcerative colitis therapy requires precise drug targeting. This study engineers a probiotic E. coli Nissle 1917 to surface‐display ANXA5, enabling specific binding to phosphatidylserine (PS) exposed on inflamed colonic epithelium for enhanced targeting and colonization.
Siqi Hua, Kaiqiang Li, Pengyou Shang, Lina Liu, Jiayu Pan, Bo Zhu, Zichun Hua   +6 more
wiley   +1 more source

Engineering Metal‐Organic‐Framework‐Based STING Nanoagonists for PROTAC‐Enhanced Cancer Chemo‐Metalloimmunotherapy

Advanced Science, EarlyView.
Schematic overview of PROTAC‐enhanced chemo‐metalloimmunotherapy, integrating senescence induction, multi‐modal STING pathway activation, PD‐L1 blockade, and immune amplification via coordinated PROTACs, metal ions, and chemotherapy to elicit robust antitumor immunity and circumvent immune evasion.
Zhenzhen Chen, Zhe Feng, Siyuan Wang, Jingjing Zhang   +3 more
wiley   +1 more source

Patient‐Specific Coculture of S. aureus and P. aeruginosa Enhances Epithelial Barrier Disruption and Virulence in CRS

International Forum of Allergy &Rhinology, EarlyView.
ABSTRACT Background Chronic rhinosinusitis (CRS) is a chronic inflammatory disease that is associated with polymicrobial infections, often involving S. aureus and P. aeruginosa. It is unclear whether the polymicrobial context plays a role in exacerbating epithelial damage, inflammation, and resistance to therapy. Methods S. aureus and P. aeruginosa (n =
Xiaohan Sun MMed, Mahnaz Ramezanpour, Jordan Hall, Emma Barry, Alkis J. Psaltis, Peter‐John Wormald, Sarah Vreugde   +6 more
wiley   +1 more source

Experimental nodular ?diabetic-like? glomerulosclerosis in guinea pigs following longacting, heterologous insulin immunization [PDF]

, 1969
A.M. Mancini, G. A. Zampa, G. D. Geminiani, A. De Vecchi   +3 more
openalex   +1 more source

Resilience to Endoplasmic Reticulum Stress Mitigates Membrane Hyperexcitability Underlying Late Disease Onset in a Murine Model of SCA6

Annals of Neurology, EarlyView.
Objective An enduring puzzle in many inherited neurological disorders is the late onset of symptoms despite expression of function‐impairing mutant protein early in life. We examined the basis for onset of impairment in spinocerebellar ataxia type 6 (SCA6), a canonical late‐onset neurodegenerative ataxia which results from a polyglutamine expansion in ...
Haoran Huang, Taylor L. Charron, Min Fu, Miranda Dunn, Deborah M. Jones, Praveen Kumar, Satoshi Ishishita, Allan‐Hermann Pool, Ashwinikumar Kulkarni, Genevieve Konopka, Vikram G. Shakkottai   +10 more
wiley   +1 more source

Reactions of the lactate dehydrogenase X-band in human sperm with homologous and heterologous antisera [PDF]

, 1967
N Ressler, Kent Stitzer, D. R. ACKERMAN
openalex   +1 more source