Results 231 to 240 of about 261,803 (304)

Engineering Streptomyces coelicolor for heterologous expression of the thiopeptide GE2270A-A cautionary tale. [PDF]

J Ind Microbiol Biotechnol
Del Carratore F, Hanko EKR, Schmidt K, Bilyk O, Ye Huang S, Iorio M, Pérez-Bonilla M, Pérez-Redondo R, Rudden M, Severi E, Tocchetti A, Sosio M, Johnson EJ, Kirkwood T, Whittall DR, Manousaki A, Genilloud O, Rodríguez-García A, Thomas GH, Donadio S, Breitling R, Takano E.   +21 more
europepmc   +1 more source

Expanding the substrate scope of a bacterial monoterpene synthase for the production of sesquiterpenoid and diterpenoid products

The FEBS Journal, EarlyView.
We have converted the only known true bacterial monoterpene synthase, cineole synthase from Streptomyces clavuligerus (bCinS, C10 substrate), to a highly competent sesquiterpene synthase (C15) with a minimum number of rational mutations. By comparison with diterpene synthases (C20), we were then able to bestow diterpene synthase activity on bCinS. This
Nicole G. H. Leferink, Joshua N. Whitehead, Linus O. Johannissen, Nigel S. Scrutton   +3 more
wiley   +1 more source

Heterologous expression of the <i>Stellaria media</i> plant defensin SmD1 in <i>Escherichia coli</i>. [PDF]

PeerJ
Qiu Y, Song Q.
europepmc   +1 more source

Plant organelle C‐to‐U RNA editing factors can operate successfully in yeast (Saccharomyces cerevisiae) as an easily amenable eukaryotic system for their functional analysis

The FEBS Journal, EarlyView.
Plant‐type pentatricopeptide repeat proteins capable of C‐to‐U RNA editing perform faithfully when expressed in a new heterologous system, the yeast Saccharomyces cerevisiae. They were tested with constitutive and inducible expression and with a set of different solubility tags. PPR56, PPR65, and PPR78 from P.
Shyam Ramanathan, Annika Hanraths, Jingchan Xie, Mareike Schallenberg‐Rüdinger, Volker Knoop   +4 more
wiley   +1 more source

Heterologous Expression of Recombinant Ginseng Tetradecapeptide in <i>Saccharomyces cerevisiae</i> and Evaluation of Its Biological Activity. [PDF]

Foods
Qi Y, Ma P, Wang P, Zhu C.
europepmc   +1 more source

Enzymatic synthesis of bioactive quinolones and (thio)coumarins by fungal type III polyketide synthases

The FEBS Journal, EarlyView.
Quinolones are valuable scaffolds for drug discovery but are rare in nature. Here, we show that two fungal enzymes, AthePKS and FerePKS, can generate 2‐quinolones and two additional heteroaromatic scaffolds. Using AthePKS, we designed an artificial enzymatic cascade towards an antimicrobial quinolone from a simple precursor and implemented it in E ...
Nika Sokolova, Angelina Osipyan, Lili Zhang, Matthew R. Groves, Sandy Schmidt, Kristina Haslinger   +5 more
wiley   +1 more source

Heterologous expression of an <i>in planta</i>-upregulated gene cluster in the wheat pathogen <i>Parastagonospora nodorum</i> establishes the biosynthesis of methylene-bridged depsides. [PDF]

Chem Sci
Nguyen HT, Sbaraini N, Bracegirdle J, Smithers L, Moggach SA, Vuong D, Lacey E, Solomon PS, Haywood J, Piggott AM, Chooi YH.   +10 more
europepmc   +1 more source

A Hanks‐type bacterial kinase, PknS, directly phosphorylates the alternative sigma factor EcfK to promote resistance to protist predation

The FEBS Journal, EarlyView.
The Xanthomonas citri Hanks‐type kinase PknS autophosphorylates and directly phosphorylates the alternative sigma factor EcfK at five residues. Besides the conserved residue T51 in the σ2 domain, phosphorylation of a residue in the linker between σ2 and σ4 is critical for EcfK activation by promoting its interaction with a positively charged pocket in ...
Lídia dos Passos Lima, Dev Sriranganadane, Daiane Laise da Silva, Natália C. Drebes Dörr, Enzo Breviglieri Sichi Mello, Caio Vinicius dos Reis, Rogério Ferreira Lourenço, José Felipe Teixeira da Silva Santos, Anita Salmazo, Brenno Wendler Miranda, Katlin B. Massirer, Rafael M. Couñago, Cristina E. Alvarez‐Martinez   +12 more
wiley   +1 more source

Identification and heterologous expression of an NRPS biosynthetic gene cluster responsible for the production of the pyrazinones Ichizinone A, B and C. [PDF]

Microb Cell Fact
Oberhäuser P, Myronovskyi M, Stierhof M, Gromyko O, Luzhetskyy A.   +4 more
europepmc   +1 more source

Impact of heterologous expression of Cannabis sativa tetraketide synthase on Phaeodactylum tricornutum metabolic profile. [PDF]

Biotechnol Biofuels Bioprod
Sene N, Gonçalves Dos Santos KC, Merindol N, Gélinas SE, Custeau A, Awwad F, Fantino E, Meddeb-Mouelhi F, Germain H, Desgagné-Penix I.   +9 more
europepmc   +1 more source