Robust expression and secretion of Xylanase1 in Chlamydomonas reinhardtii by fusion to a selection gene and processing with the FMDV 2A peptide. [PDF]
PLoS ONE, 2012 Microalgae have recently received attention as a potential low-cost host for the production of recombinant proteins and novel metabolites. However, a major obstacle to the development of algae as an industrial platform has been the poor expression of ...
Beth A Rasala +5 more
doaj +1 more source
Journal of Fungi, 2023 Aspergillus niger is a primary cell factory for food-grade protein (enzyme) production due to its strong protein secretion capacity and unique safety characteristics. The bottleneck issue for the current A.
Ke Li +4 more
doaj +1 more source
Secretion and assembly of functional mini-cellulosomes from synthetic chromosomal operons in Clostridium acetobutylicum ATCC 824. [PDF]
, 2013 Background: Consolidated bioprocessing (CBP) is reliant on the simultaneous enzyme production, saccharification of biomass, and fermentation of released sugars into valuable products such as butanol. Clostridial species that produce butanol are, however,
Bolam, DN +7 more
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18-Hydroxydolabella-3,7-diene synthase – a diterpene synthase from Chitinophaga pinensis
Beilstein Journal of Organic Chemistry, 2017 The product obtained in vitro from a diterpene synthase encoded in the genome of the bacterium Chitinophaga pinensis, an enzyme previously reported to have germacrene A synthase activity during heterologous expression in Escherichia coli, was identified ...
Jeroen S. Dickschat +4 more
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Production of Recombinant Biopharmaceuticals in Chlamydomonas reinhardtii
International Journal of Plant Biology, 2022 This review aimed to present Chlamydomonas reinhardtii as an alternative for heterologous protein production, especially for biopharmaceuticals, and its general characteristics when compared with other expression systems. The need to produce heterologous
Cesar Andres Diaz Arias +5 more
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Characterization of the Arsenate Respiratory Reductase from Shewanella sp. Strain ANA-3 [PDF]
, 2008 Microbial arsenate respiration contributes to the mobilization of arsenic from the solid to the soluble phase in various locales worldwide. To begin to predict the extent to which As(V) respiration impacts arsenic geochemical cycling, we characterized ...
Keeffe, Jennifer R. +2 more
core +2 more sources
mBio, 2017 Heterologous expression has become a powerful tool for studying microbial biosynthetic gene clusters (BGCs). Here, we extend the transformation-associated recombination cloning and heterologous expression platform for microbial BGCs to include Gram ...
Jia Jia Zhang +4 more
doaj +1 more source
Pass-back chain extension expands multimodular assembly line biosynthesis. [PDF]
, 2020 Modular nonribosomal peptide synthetase (NRPS) and polyketide synthase (PKS) enzymatic assembly lines are large and dynamic protein machines that generally effect a linear sequence of catalytic cycles.
Huan, Tao +5 more
core
Spores of Clostridium engineered for clinical efficacy and safety cause regression and cure of tumors in vivo. [PDF]
, 2014 Spores of some species of the strictly anaerobic bacteria Clostridium naturally target and partially lyse the hypoxic cores of tumors, which tend to be refractory to conventional therapies. The anti-tumor effect can be augmented by engineering strains to
Dubois, L +10 more
core +7 more sources
Expression systems for industrial Gram-positive bacteria with low guanine and cytosine content [PDF]
, 1997 Recent years have seen an increase in the development of gene expression systems for industrial Gram-positive bacteria with low guanine and cytosine content that belong to the genera Bacillus, Clostridium, Lactococcus, Lactobacillus, Staphylococcus and ...
Kleerebezem, Michiel, +2 more
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