Results 41 to 50 of about 145,939 (263)

Plant‐Produced Viral Nanoparticles Decorated with Nanobodies Against HER2 Improve Retention and Recruitment of Immune Cells in Solid Tumors

Advanced Healthcare Materials, EarlyView.
Potato virus X is a filamentous RNA plant virus that can be engineered into a molecular tool for cancer therapy. We produced genetically‐encoded virus‐derived nanoparticles decorated with nanobodies targeting cancer cell receptors, epidermal growth factor receptor (EGFR) and human epidermal growth factor 2 (HER2).
Enrique Lozano‐Sanchez, Miguel A. Moreno‐Gonzalez, Fernando Merwaiss, Nicole F. Steinmetz, José‐Antonio Daròs   +4 more
wiley   +1 more source

Integrating Vibrio natriegens for Photon Manipulation in Living Lighting Devices

Advanced Materials, EarlyView.
Rationalization of genetic and material engineering tools has enabled the engineering of fluorescent Vibrio natriegens (V. natriegens) to fabricate straightforward, highly emissive and stable V. natriegens‐silicones color filters for the first red‐emitting bacteria‐hybrid light‐emitting diodes. This pinpoints V. natriegens as a high‐performance protein
Stephanie Willeit, Maurice Hädrich, Philippe‐Quentin Liss, Valeria Rodriguez Espinoza, Nicklas Maximilian Foerster, Bastian Blombach, Rubén D. Costa   +6 more
wiley   +1 more source

Bacteria and Fungi Synergistically Reprogram Flavonoid Metabolites in the Pericarp of Citrus Reticulata 'Chachi' During Storage

Advanced Science, EarlyView.
The authors employed widely targeted metabolomics, microbial amplicon sequencing, and fermentation assays to investigate the microbiome 's influence on PCRC 's (pericarp of Citrus reticulata ' Chachi ') flavonoid profile over 0–19 years of storage. Based on the correlation analysis, solid‐state and liquid‐state fermentation, and catalytic activity ...
Jianmu Su, Sisheng Zhang, Lidi Liang, Haiyi Bai, Mei Bai, Hanjun He, Yayu Wang, Huan Liu, Xiangxiu Liang, Yu Sun, Hong Wu   +10 more
wiley   +1 more source

Use of the mCherry fluorescent protein to optimize the expression of class I lanthipeptides in Escherichia coli

Microbial Cell Factories, 2023
Background Lanthipeptides are a rapidly expanding family of ribosomally synthesized and post-translationally modified natural compounds with diverse biological functions.
Winschau F Van Zyl, Anton D. Van Staden, Leon M T. Dicks, Marla Trindade   +3 more
doaj   +1 more source

Construction and testing of Yarrowia lipolytica recombinant protein expression chassis cells based on the high-throughput screening and secretome

Microbial Cell Factories, 2023
Background In the recombinant protein market with broad economic value, the rapid development of synthetic biology has made it necessary to construct an efficient exocrine expression system for the different heterologous proteins.
Siqian Yu, Ge Zhang, Qi Liu, Yingping Zhuang, Zongjie Dai, Jianye Xia   +5 more
doaj   +1 more source

Heterologous expression of heterotrophic nitrification genes [PDF]

Microbiology, 1997
Paracoccus denitrificans is a heterotrophic organism capable of oxidizing ammonia to nitrite during growth on an organic carbon and energy source. This pathway, termed heterotrophic nitrification, requires the concerted action of an ammonia monooxygenase (AMO) and hydroxylamine oxidase (HAO).
Crossman, Lisa C., Moir, James W.B., Enticknap, Julie J., Richardson, David J., Spiro, Stephen   +4 more
openaire   +3 more sources

Pre‐Encoded IFN‐I Sensitivity Exacerbates Memory T Cell Senescence in Solid Tumors

Advanced Science, EarlyView.
Type I interferon (IFN‐I) signaling promotes p21‐dependent cell cycle arrest in senescent tumor‐specific memory T cells, resulting in poor proliferative responses and solid tumor regression during cancer vaccination. Conversely, IFNα/β receptor blockade reinvigorates T cell proliferation to regress solid tumors and is more effective with increasing ...
Andrew Nguyen, Scott R Walsh, Li Deng, Lan Chen, Yonghong Wan   +4 more
wiley   +1 more source

Heterologous protein expression is enhanced by harmonizing the codon usage frequencies of the target gene with those of the expression host. [PDF]

PLoS ONE, 2008
Synonymous codon replacement can change protein structure and function, indicating that protein structure depends on DNA sequence. During heterologous protein expression, low expression or formation of insoluble aggregates may be attributable to ...
Evelina Angov, Collette J Hillier, Randall L Kincaid, Jeffrey A Lyon   +3 more
doaj   +1 more source

Expression of Lectins in Heterologous Systems [PDF]

International Journal of Molecular Sciences, 2018
Lectins are proteins that have the ability to recognize and bind in a reversible and specific way to free carbohydrates or glycoconjugates of cell membranes. For these reasons, they have been extensively used in a wide range of industrial and pharmacological applications.
Dania Martínez-Alarcón, Alejandro Blanco-Labra, Teresa García-Gasca   +2 more
openaire   +2 more sources

Attenuation of Influenza a Virus into Live Vaccines Through C‐End Degrons

Advanced Science, EarlyView.
Naturally occurring C‐end degrons are exploited to design proteolysis‐targeting live attenuated influenza vaccines. C‐end degron tagging of viral M1 protein promotes proteasome‐dependent degradation, resulting in robust attenuation in host cells while permitting scalable production in engineered cells.
Ping Wang, Le Li, Yunfang Chen, Le Tong, Zhen Li, Rong Yu, Quan Shen, Qikai Wang, Jihuan Hou, Qisi Zhang, Xu Si, Ning Wang, Demin Zhou, Wen‐xia Tian, Longlong Si   +14 more
wiley   +1 more source