Results 51 to 60 of about 261,803 (304)

Potential therapeutic targeting of BKCa channels in glioblastoma treatment

Molecular Oncology, EarlyView.
This review summarizes current insights into the role of BKCa and mitoBKCa channels in glioblastoma biology, their potential classification as oncochannels, and the emerging pharmacological strategies targeting these channels, emphasizing the translational challenges in developing BKCa‐directed therapies for glioblastoma treatment.
Kamila Maliszewska‐Olejniczak, Karolina Pytlak, Sandra Jaworowska, Bogusz Kulawiak, Piotr Bednarczyk   +4 more
wiley   +1 more source

Understanding the Wolbachia-mediated inhibition of arboviruses in mosquitoes: progress and challenges [PDF]

, 2014
Arthropod-borne viruses (arboviruses) pose a considerable threat to human and animal health, yet effective control measures have proven difficult to implement, and novel means of controlling their replication in arthropod vectors, such as mosquitoes, are
Dietrich, Isabelle, Kohl, Alain, Rainey, Stephanie M., Shah, Pranav   +3 more
core   +1 more source

Dammarenediol II enhances etoposide‐induced apoptosis by targeting O‐GlcNAc transferase and Akt/GSK3β/mTOR signaling in liver cancer

Molecular Oncology, EarlyView.
Etoposide induces DNA damage, activating p53‐dependent apoptosis via caspase‐3/7, which cleaves PARP1. Dammarenediol II enhances this apoptotic pathway by suppressing O‐GlcNAc transferase activity, further decreasing O‐GlcNAcylation. The reduction in O‐GlcNAc levels boosts p53‐driven apoptosis and influences the Akt/GSK3β/mTOR signaling pathway ...
Jaehoon Lee, Byung‐Cheol Han, Gi‐Bang Koo, Jihye Park, Mijin Kwon, Young Bin Park, Jae‐Mun Choi, Seung‐Ho Lee, Sangho Roh   +8 more
wiley   +1 more source

A novel platform for the production of nonhydroxylated gelatins based on the methylotrophic yeast Hansenula polymorpha [PDF]

, 2007
The use of yeast as a host for heterologous expression of proteins that are normally derived from animal tissue is a promising way to ensure defined products that are devoid of potential harmful animal side products.
Boer, Arjo L. de,, Geerlings, Torsten H.,, Klei, Ida J. van der,, Lunenborg, Marcel G.J.,, Veenhuis, Marten,   +4 more
core   +2 more sources

Combinatorial engineering for heterologous gene expression [PDF]

Bioengineered, 2013
Tools for strain engineering with predictable outcome are of crucial importance for the nascent field of synthetic biology. The success of combining different DNA biological parts is often restricted by poorly understood factors deriving from the complexity of the systems.
Friederike, Zwick, Rahmi, Lale, Svein, Valla   +2 more
openaire   +2 more sources

Rethinking plastic waste: innovations in enzymatic breakdown of oil‐based polyesters and bioplastics

FEBS Open Bio, EarlyView.
Plastic pollution remains a critical environmental challenge, and current mechanical and chemical recycling methods are insufficient to achieve a fully circular economy. This review highlights recent breakthroughs in the enzymatic depolymerization of both oil‐derived polyesters and bioplastics, including high‐throughput protein engineering, de novo ...
Elena Rosini, Nicolò Antonelli, Gianluca Molla   +2 more
wiley   +1 more source

Heterologous Expression and Transglycosylation of Acarviosyltransferase [PDF]

Shipin Kexue
In order to further investigate the structural properties and catalytic function of acarviosyltransferase (ATase), a key enzyme in the biosynthesis of acarbose, its gene (acbD) was cloned from Actinoplanes sp.
XUE Zhenglian, WANG Yuqing, LI Chuang, LI Dandan, ZHU Sibao, LI Xiangfei
doaj   +1 more source

The Generation of Successive Unmarked Mutations and Chromosomal Insertion of Heterologous Genes in Actinobacillus pleuropneumoniae Using Natural Transformation [PDF]

, 2014
We have developed a simple method of generating scarless, unmarked mutations in Actinobacillus pleuropneumoniae by exploiting the ability of this bacterium to undergo natural transformation, and with no need to introduce plasmids encoding recombinases or
A Maas, A Maas, Alexander W. Tucker, Andrew N. Rycroft, B Zhan, Brendan W. Wren, C O'Neill, Denise M. Soares-Bazzolli, Duncan J. Maskell, EA Izano, FF Buettner, G Li, Glenn Francis Browning, H Maughan, I Jacobsen, I Jacobsen, Janine T. Bossé, JT Bossé, JT Bossé, JT Bossé, JT Bossé, JT Bossé, JT Bossé, L Lin, LM Mullen, M Copass, M Ramjeet, N Baltes, N Baltes, N Baltes, N Baltes, N Baltes, Paul R. Langford, PJ Blackall, R Jansen, RJ Redfield, RM Jones, RV Joshi, SJ Foote, T Ali, TJ Inzana, W Oswald, W Tonpitak, W Tonpitak, WW Fung, Yanwen Li, Z Xu   +46 more
core   +5 more sources

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

FEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq, Nestor G. Casillas‐Vega, Alma Gomez‐Loredo, Xristo Zarate   +3 more
wiley   +1 more source

Cloning and expression of Burkholderia polyyne biosynthetic gene clusters in Paraburkholderia hosts provides a strategy for biopesticide development

Microbial Biotechnology, 2022
Burkholderia have potential as biocontrol agents because they encode diverse biosynthetic gene clusters (BGCs) for a range of antimicrobial metabolites.
Yoana D. Petrova, Jinlian Zhao, Gordon Webster, Alex J. Mullins, Katherine Williams, Amal S. Alswat, Gregory L. Challis, Andy M. Bailey, Eshwar Mahenthiralingam   +8 more
doaj   +1 more source