Results 11 to 20 of about 7,225 (221)

Identification and characterization of mature β-hexosaminidases associated with human placenta lysosomal membrane

Bioscience Reports, 2008
International audienceβ-Hexosaminidase is a soluble glycohydrolase involved in glycoconjugate degradation into lysosomes, nevertheless its localization has also been described in cytosol and plasma membrane.
Ahn, Alessandro Magini, Andrej Hasilik, Bordier, Bradford, Brunella Tancini, Carla Emiliani, Cattaneo, Chen, Chen, Cordero, De Duve, Diettrich, Emiliani, Emiliani, Eskelinen, Fujiki, Gao, Goi, Gravel, Hakamori, Hakomori, Hakomori, Hakomori, Hasilik, Laemmli, Lambert, Lemieux, Leonova, Liang, Lorena Urbanelli, Lukong, Mahuran, Mahuran, Mark, Martino, Massaccesi, Mencarelli, Orlacchio, Papini, Pasini, Proia, Proia, Reczek, Schröder, Simona Mencarelli, Valaperta, Virginia Ciccarone, Wang   +48 more
core   +3 more sources

Posttranslational modifications of heterologous proteins expressed in Nicotiana benthamiana. [PDF]

Plant Biotechnol J
Summary The success of Nicotiana benthamiana as a workhorse for heterologous protein production is closely linked to its accessibility and tolerance to genetic manipulation, allowing efficient engineering of posttranslational protein modifications (PTMs) that are critical for the function and stability of heterologous proteins.
Göritzer K, Kallolimath S, Strasser R.
europepmc   +2 more sources

Highly efficient transgene-free ErCas12a RNP-protoplast genome editing and single-cell regeneration in Nicotiana benthamiana for glyco-engineering. [PDF]

Plant Biotechnol J
Summary Nicotiana benthamiana serves as a unique platform for biopharmaceutical production, offering advantages such as efficient and scalable protein synthesis. In addition, custom N‐glycans can be engineered on biopharmaceutical glycoproteins. Yet, plant‐native glycosyltransferases and glycoside hydrolases need to be removed to prevent undesired ...
Blumberg LC, Bakker GM, van der Kaaij A, Gariépy É, van de Geest H, Slootweg EJ, Los FCO, Westerhof LB, Wilbers RHP.   +8 more
europepmc   +2 more sources

Damage of tubule cells in diabetic nephropathy type 2: Urinary N-acetyl-β-D-glucosaminidasis and γ-glutamil-transferasis [PDF]

Vojnosanitetski Pregled, 2007
Background/Aim. A damage of tubular epithelial cells is followed by the release of cell enzymes and production of proinflammatory compounds, which lead to the tubulointerstitial damage.
Vlatković Vlastimir, Stojimirović Biljana, Obrenović Radmila   +2 more
doaj   +1 more source

Chitin Degradation Machinery and Secondary Metabolite Profiles in the Marine Bacterium Pseudoalteromonas rubra S4059

Marine Drugs, 2021
Genome mining of pigmented Pseudoalteromonas has revealed a large potential for the production of bioactive compounds and hydrolytic enzymes. The purpose of the present study was to explore this bioactivity potential in a potent antibiotic and enzyme ...
Xiyan Wang, Thomas Isbrandt, Mikael Lenz Strube, Sara Skøtt Paulsen, Maike Wennekers Nielsen, Yannick Buijs, Erwin M. Schoof, Thomas Ostenfeld Larsen, Lone Gram, Sheng-Da Zhang   +9 more
doaj   +1 more source

Automated assay of hexosaminidases in serum.

Clinical Chemistry, 1973
Abstract In serum there are two classes of hexosaminidase. One of these is heat-labile and is inactive in children with Tay—Sachs disease. Serum from heterozygotes for Tay—Sachs disease has half the normal activity of the heat-labile enzyme.
J. Lowden, M. Skomorowski, F. Henderson, M. Kaback   +3 more
semanticscholar   +3 more sources

Characterization of inducible models of Tay-Sachs and related disease. [PDF]

PLoS Genetics, 2012
Tay-Sachs and Sandhoff diseases are lethal inborn errors of acid β-N-acetylhexosaminidase activity, characterized by lysosomal storage of GM2 ganglioside and related glycoconjugates in the nervous system.
Timothy J Sargeant, Deborah J Drage, Susan Wang, Apostolos A Apostolakis, Timothy M Cox, M Begoña Cachón-González   +5 more
doaj   +1 more source

Human kidney hexosaminidase A and hexosaminidase B form a complex [PDF]

European Journal of Biochemistry, 1991
The isolation and purification of human kidney hexosaminidases A and B was carried out. Regulation of the supramolecular organization and catalytic activity of hexosaminidases was investigated in the bis(2‐ethylhexyl)sulphosuccinate reversed micellar system modeling the enzyme microenvironment in the lysosomes. It was shown that hexosaminidases A and B
A V, Pshezhetsky, O A, Buneeva, V M, Dvorkin   +2 more
openaire   +2 more sources

Bicistronic lentiviral vector corrects β-hexosaminidase deficiency in transduced and cross-corrected human Sandhoff fibroblasts

Neurobiology of Disease, 2005
Sandhoff disease is an autosomal recessive neurodegenerative disease characterized by a GM2 ganglioside intralysosomal accumulation. It is due to mutations in the β-hexosaminidases β-chain gene, resulting in a β-hexosaminidases A (αβ) and B (ββ ...
Audrey Arfi, Christophe Bourgoin, Luisa Basso, Carla Emiliani, Brunella Tancini, Vanna Chigorno, Yu-Teh Li, Aldo Orlacchio, Livia Poenaru, Sandro Sonnino, Catherine Caillaud   +10 more
doaj   +1 more source

O-GlcNAcase:promiscuous hexosaminidase or key regulator of O-GlcNAc signalling? [PDF]

, 2014
O-GlcNAc signaling is regulated by an opposing pair of enzymes: O-GlcNAc transferase installs and O-GlcNAcase (OGA) removes the modification from proteins.
Banerjee, Butkinaree, Cantarel, Cecioni, Cetinba, Comtesse, Copeland, Dennis, Dong, Dorfmueller, Dorfmueller, Drougat, Forsythe, Francisco, Gao, Gong, Graham, Guinez, Hanover, Hart, Hayakawa, He, He, Heckel, Hilgers, Housley, Howerton, Hu, Itkonen, Keembiyehetty, Knapp, Laczy, Lefebvre, Lepage, Libina, Liu, Macauley, Macauley, Macauley, Macauley, Macauley, Mahuran, Martin, Minami, Müller, Nagel, Olszewski, Ostrowski, Pathak, Penton, Radermacher, Rahman, Rao, Rao, Robinson, Ruan, Ruan, Schimpl, Schimpl, Schultz, Sekine, Shen, Slawson, Speakman, Stryke, Swallow, Tews, Toleman, Toleman, Torres, Wang, Wang, Webster, Wells, Whisenhunt, Whitworth, Yang, Yu, Yuzwa, Yuzwa, Zachara, Zachara, Zhu   +82 more
core   +3 more sources