Results 1 to 10 of about 1,401 (83)

Structural analysis and functional evaluation of the disordered ß–hexosyltransferase region from Hamamotoa (Sporobolomyces) singularis [PDF]

Frontiers in Bioengineering and Biotechnology, 2023
Hamamotoa (Sporobolomyces) singularis codes for an industrially important membrane bound ß-hexosyltransferase (BHT), (BglA, UniprotKB: Q564N5) that has applications in the production of natural fibers such as galacto-oligosaccharides (GOS) and natural sugars found in human milk.
Suzanne F. Dagher, Asmita Vaishnav, Christopher B. Stanley, Flora Meilleur, Flora Meilleur, Brian F. P. Edwards, José M. Bruno-Bárcena   +6 more
semanticscholar   +5 more sources

Biochemical and Biophysical Characterization of the Sialyl-/Hexosyltransferase Synthesizing the Meningococcal Serogroup W135 Heteropolysaccharide Capsule [PDF]

Journal of Biological Chemistry, 2013
Neisseria meningitidis (Nm) is a leading cause of bacterial meningitis and sepsis. Crucial virulence determinants of pathogenic Nm strains are the polysaccharide capsules that support invasion by hindering complement attack. In NmW-135 and NmY the capsules are built from the repeating units (→ 6)-α-D-Gal-(1 → 4)-α-Neu5Ac-(2 →)n and (→ 6)-α-D-Glc-(1 → 4)
Romanow, Angela, Haselhorst, Thomas, Stummeyer, Katharina, Claus, Heike, Bethe, Andrea, Muehlenhoff, Martina, Vogel, Ulrich, von Itzstein, Mark, Gerardy-Schahn, Rita   +8 more
semanticscholar   +6 more sources

Heterologous Expression of a Bioactive β-Hexosyltransferase, an Enzyme Producer of Prebiotics, from Sporobolomyces singularis [PDF]

Applied and Environmental Microbiology, 2013
ABSTRACTGalacto-oligosaccharides (GOS) are indigestible dietary fibers that are able to reach the lower gastrointestinal tract to be selectively fermented by health-promoting bacteria. In this report, we describe the heterologous expression of an optimized synthetically produced version of the β-hexosyltransferase gene (Bht) fromSporobolomyces ...
Suzanne F, Dagher, M Andrea, Azcarate-Peril, José M, Bruno-Bárcena   +2 more
semanticscholar   +4 more sources

A novel N-terminal region of the membrane β-hexosyltransferase: its role in secretion of soluble protein by Pichia pastoris

Microbiology, 2016
The β-hexosyltransferase (BHT) from Sporobolomyces singularis is a membrane-bound enzyme that catalyses transgalactosylation reactions to synthesize galacto-oligosaccharides (GOSs). To increase the secretion of the active soluble version of this protein, we examined the uncharacterized novel N-terminal region (amino acids 1-110), which included two ...
Suzanne F, Dagher, José M, Bruno-Bárcena   +1 more
semanticscholar   +5 more sources

A conserved motif in the hexosyltransferases [PDF]

Molecular Microbiology, 1999
Henderson, Ian R., Nataro, James P.
semanticscholar   +5 more sources

In silico Approach for Unveiling the Glycoside Hydrolase Activities in Faecalibacterium prausnitzii Through a Systematic and Integrative Large-Scale Analysis

Frontiers in Microbiology, 2019
This work presents a novel in silico approach to the prediction and characterization of the glycolytic capacities of the beneficial intestinal bacterium Faecalibacterium prausnitzii. Available F.
Guillermo Blanco, Guillermo Blanco, Guillermo Blanco, Borja Sánchez, Florentino Fdez-Riverola, Florentino Fdez-Riverola, Florentino Fdez-Riverola, Abelardo Margolles, Anália Lourenço, Anália Lourenço, Anália Lourenço, Anália Lourenço   +11 more
doaj   +2 more sources

Morpho-biochemical characterization of a RIL population for seed parameters and identification of candidate genes regulating seed size trait in lentil (Lens culinaris Medik.)

Frontiers in Plant Science, 2023
The seed size and shape in lentil (Lens culinaris Medik.) are important quality traits as these influences the milled grain yield, cooking time, and market class of the grains. Linkage analysis was done for seed size in a RIL (F5:6) population derived by
Haragopal Dutta, Shivaprasad K. M., M. Aski, Gyan P Mishra, S. K. Sinha, D. Vijay, M. C T, Shouvik Das, P. Pawar, D. Mishra, Ashutosh Kumar Singh, Atul Kumar, Kuldeep Tripathi, R. Kumar, Sanjeev Gupta, Shiv Kumar, H. K. Dikshit   +16 more
semanticscholar   +1 more source

Convergent copy number increase of genes associated with freshwater colonization in fishes

Philosophical Transactions of the Royal Society of London. Biological Sciences, 2022
Copy number variation (CNV) can cause phenotypic changes. However, in contrast to amino acid substitutions and cis-regulatory changes, little is known about the functional categories of genes in which CNV is important for adaptation to novel environments.
Asano Ishikawa, Shun Yamanouchi, W. Iwasaki, J. Kitano   +3 more
semanticscholar   +1 more source

Identification of Main-Effect and Environmental Interaction QTL and Their Candidate Genes for Drought Tolerance in a Wheat RIL Population Between Two Elite Spring Cultivars

Frontiers in Genetics, 2021
Understanding the genetics of drought tolerance can expedite the development of drought-tolerant cultivars in wheat. In this study, we dissected the genetics of drought tolerance in spring wheat using a recombinant inbred line (RIL) population derived ...
S. Rabbi, Ajay Kumar, Sepehr Mohajeri Naraghi, S. Sapkota, M. Alamri, E. Elias, S. Kianian, Raed I. Seetan, A. Missaoui, S. Solanki, M. Mergoum   +10 more
semanticscholar   +1 more source

Identification of Subsets of Enteroaggregative Escherichia coli Associated with Diarrheal Disease among Under 5 Years of Age Children from Rural Gambia

American Journal of Tropical Medicine and Hygiene, 2017
. Enteroaggregative Escherichia coli (EAEC) cause acute and persistent diarrhea, mostly in children worldwide. Outbreaks of diarrhea caused by EAEC have been described, including a large outbreak caused by a Shiga toxin expressing strain.
U. Ikumapayi, N. Boisen, M. J. Hossain, Modupeh Betts, Modou Lamin, D. Saha, B. Kwambana-Adams, M. Dione, R. Adegbola, A. Roca, J. Nataro, M. Antonio   +11 more
semanticscholar   +1 more source