Results 21 to 30 of about 23,384,570 (255)

Development and validation of an HPLC-UV method for purity determination of DNA

Medicine Science, 2023
Determination of the concentration and the purity of DNAs is crucial for measuring the DNA copy number since it will influence further DNA analysis such as digital PCR (dPCR) and next-generation sequencing (NGS).
Merve Oztug, Burhanettin Yalcinkaya
doaj   +1 more source

Measurement of stable isotopic enrichment and concentration of long-chain fatty acyl-carnitines in tissue by HPLC-MS

Journal of Lipid Research, 2006
We have developed a new method for the simultaneous measurements of stable isotopic tracer enrichments and concentrations of individual long-chain fatty acyl-carnitines in muscle tissue using ion-pairing high-performance liquid chromatography ...
Dayong Sun, Melanie G. Cree, Xiao-jun Zhang, Elisabet Bøersheim, Robert R. Wolfe   +4 more
doaj   +1 more source

New HPLC method for determination of fluvastatin anti-hyperlipidemic drug in bulk and pharmaceutical dosage using simvastatin as internal standard

Drug Analytical Research, 2023
A new, and reliable RP–HPLC method has been investigated and validated for the assay of fluvastatin sodium (FVS) in pharmaceutical capsules. The chromatographic method was carried out using an ODS Hypersil C18 column (250×4.6 mm i.d., 5 µm particle size)
Safwan Ashour, Mouhammed Khateeb
doaj   +1 more source

Quantification of voriconazole in plasma by liquid chromatography-tandem mass spectrometry [PDF]

, 2005
A convenient liquid chromatography-tandem mass spectrometry method for the quantification of the triazole antifungal agent voriconazole in plasma samples is described. Fenbuconazole is used as an internal standard.
Spöhrer, Ute, Schiel, Xaver, Vogeser, Michael   +2 more
core   +1 more source

Degradation mechanism of the von Willebrand factor A2 domain by nattokinase

FEBS Letters, EarlyView.
Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto, Masatomo So, Ayako Furukawa, Kenji Sugase   +3 more
wiley   +1 more source

ABL kinase‐dependent phosphorylation of SH proteins promotes their direct interaction with CRK family SH2 domains

FEBS Letters, EarlyView.
CT10 regulator of kinase (CRK) and CRK‐Like (CRKL) are signaling adaptors driving cell adhesion, motility, differentiation, and proliferation. SH2‐domain containing (SH) proteins are enriched in YXXP motifs which when phosphorylated create preferred binding sites for CRK family SH2 domains.
Phoebe M. Cousens, Brendan W. Chandler, Anna M. Schmoker, Jaye L. Weinert, Charlotte A. Kearns, Warren T. Yacawych, Amila Šemić, Alicia M. Ebert, Bryan A. Ballif   +8 more
wiley   +1 more source

Determination of stigmasterol hydroperoxides using high-performance liquid chromatography-mass spectrometry with atmospheric pressure chemical ionization

Czech Journal of Food Sciences, 2004
A new specific method using high-performance liquid chromatography-mass spectrometry (HPLC-MS) for the detection of stigmasterol hydroperoxides was developed.
S. Kemmo, V. Ollilainen, A.-M. Lampi, V. Piironen   +3 more
doaj   +1 more source

Partial depletion of plasminogen activator inhibitor‐1 decreases subcutaneous fat cell hypertrophy and liver cholesterol in high‐fat‐fed female mice

FEBS Letters, EarlyView.
Obesity raises blood levels of PAI‐1, a protein linked to metabolic dysfunction‐associated steatotic liver disease in people with obesity. In female mice fed a high‐fat diet, partially lowering PAI‐1 led to smaller subcutaneous fat cells and lower liver cholesterol, without changing body weight or insulin sensitivity.
Claudia E. Ramirez Bustamante, Robert Sharp, Pradip K. Saha, Elizabeth S. Anaya, Elizabeth L. Lieu, Evelyn L. de Groot, Pooja Patel, Mi Jeong Heo, Aaron R. Cox, Kang Ho Kim, Sean M. Hartig   +10 more
wiley   +1 more source

New trends in liquid chromatography and their utilization in analysis of beer and brewery raw materials. Part 3. Comparison of HPLC and UHPLC determination of α- and β-acids.

Kvasný průmysl, 2012
The traditional method of High Performance Liquid Chromatography (HPLC) was compared with the currently increasingly used Ultra- High Performance Liquid Chromatography (UHPLC) on a representative group of 11 hop samples.
Marie JURKOVÁ, Pavel ČEJKA, Jana OLŠOVSKÁ   +2 more
doaj   +1 more source

Emerging experimental and computational methods for studying redox‐regulated structural transitions

FEBS Letters, EarlyView.
Redox reactions can reshape proteins and alter how they behave in cells, with important consequences for health and disease. This review explores emerging experimental and computational approaches for discovering these redox‐sensitive protein switches, revealing their structural effects, and predicting their behavior, opening new opportunities to ...
Tasneem Rass, Dana Reichmann, Gábor Erdős   +2 more
wiley   +1 more source