Results 31 to 40 of about 483,779 (311)

New trends in liquid chromatography and their utilization in analysis of beer and brewery raw materials. Part 3. Comparison of HPLC and UHPLC determination of α- and β-acids.

open access: yesKvasný průmysl, 2012
The traditional method of High Performance Liquid Chromatography (HPLC) was compared with the currently increasingly used Ultra- High Performance Liquid Chromatography (UHPLC) on a representative group of 11 hop samples.
Marie JURKOVÁ   +2 more
doaj   +1 more source

Integrated Nano Liquid Chromatography System On-a-Chip [PDF]

open access: yes, 2006
Integrated liquid chromatography (LC) chips are valued because of their significant advantages over conventional systems. However, they are very challenging to build due to the high complexity of LC systems and the need for high-level integration of many
He, Qing
core   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

open access: yesFEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa   +5 more
wiley   +1 more source

Analysis of total urinary catecholamines by liquid chromatography: methodology, routine experience and clinical interpretations of results

open access: yes, 1983
A simple routine method is described for simultaneous assay of total urinary adrenaline, noradrenaline and dopamine. The catecholamines are pre-purified on a small ion-exchange column, separated by reversed phase ion-pair liquid chromatography, and are ...
Schleicher, E. D.   +2 more
core   +1 more source

Degradation mechanism of the von Willebrand factor A2 domain by nattokinase

open access: yesFEBS Letters, EarlyView.
Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto   +3 more
wiley   +1 more source

Analysis of sphingoid bases by reversed-phase high performance liquid chromatography.

open access: yesJournal of Lipid Research, 1983
A reversed-phase high performance liquid chromatography (HPLC) technique was developed for the analysis of sphingoid bases as their biphenylcarbonyl derivatives.
F B Jungalwala   +3 more
doaj   +1 more source

Temperature-Controlled Microchip Liquid Chromatography System [PDF]

open access: yes, 2006
High-performance liquid chromatography (HPLC) is one of the most important analytical tools heavily used in the fields of chemistry, biotechnology, pharmaceutics, and the food industry.
Shih, Victor-Chi-Yuan
core   +1 more source

An unexpected alternative viologen electron mediator site in tungsten‐containing formate dehydrogenase

open access: yesFEBS Letters, EarlyView.
An unexpected alternative interaction site for ethyl viologen was identified in formate dehydrogenase 1 from Methylorubrum extorquens. Combined mutagenesis, kinetic analysis, and docking revealed that aromatic residues near an iron–sulfur cluster enable flavin mononucleotide‐independent electron transfer, offering a framework for engineering improved ...
Eleni G. Poloniataki, Yong Hwan Kim
wiley   +1 more source

Analysis of aminophospholipid molecular species by high performance liquid chromatography.

open access: yesJournal of Lipid Research, 1989
A new method is described for the separation of individual molecular species of the aminophospholipids, phosphatidylethanolamine and phosphatidylserine. Trinitrobenzene-sulfonic acid was used to derivatize both aminophospholipids and the derivatives were
F Hullin, H Y Kim, N Salem, Jr
doaj   +1 more source

Quantification of norisoboldine in Linderae Radix by ultra-performance liquid chromatography and high performance liquid chromatography with UV detection

open access: yes, 2012
Norisoboldine is an alkaloid and has been identified as the major active component in Linderae Radix. A novel method for quantitative analysis of norisoboldine in Linderae Radix using ultra performance liquid chromatography (UPLC) with UV detection was ...
Chou, G.-X.   +4 more
core   +1 more source

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