High-speed counter-current chromatography of apple procyanidins
Journal of Chromatography A, 2000Apple procyanidins were separated by high-speed counter-current chromatography using a type-J multilayer coil planet centrifuge. Several two-phase solvent systems with a wide range of hydrophobicities from a non-polar hexane system to polar n-butanol systems were evaluated their performance in terms of the partition coefficient and the retention of the
Y, Shibusawa +5 more
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Axial dispersion coefficient in high-speed counter-current chromatography
Journal of Chromatography A, 2009Experimental measurements of axial dispersion coefficients in high-speed counter-current chromatography have been carried out in the single-phase and two-phase modes. Axial dispersion coefficients were calculated from the residence time distribution curve (or the elution profile).
Zhao, Chun-Xia +2 more
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Sample capacity in preparative high-speed counter-current chromatography
Journal of Chromatography A, 2007High-speed counter-current chromatography (HSCCC) is a versatile technique in preparative separation and purification of pure compounds from complex matrices. As a preparative chromatography, there is a need to maximize the column production. Based on the plate theory of Van Deemter, the effect of the sample load on the separation was investigated in a
Zhao, Chun-Xia, He, Chao-Hong
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Improved spiral disk assembly for high-speed counter-current chromatography
Journal of Chromatography A, 2003The spiral disk for type-J high-speed counter-current chromatography (HSCCC) has been improved by placing short segments of PTFE (tetrafluoroethylene) tubing into the channel at regular intervals. The best results were obtained with a four-channel spiral column using 600 spacers, which significantly improved stationary phase retention and partition ...
Yoichiro, Ito +4 more
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Separation of spiramycin components using high-speed counter-current chromatography
Journal of Chromatography A, 2000High-speed counter-current chromatography was successfully applied to the separation of the main components of spiramycin. A 25-mg quantity of sample was separated using a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (3:6:5:5), and the fractions were analyzed by high-performance liquid chromatography and fast atom ...
H, Oka, K, Harada, M, Suzuki, Y, Ito
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Preparative separation of pyrrolizidine alkaloids by high-speed counter-current chromatography
Journal of Chromatography A, 1996We have applied a high-speed counter-current chromatography (CCC) technique to the separation and purification of pyrrolizidine alkaloids from Amsinckia tessellata, Symphytum spp., Trichodesma incanum (Boraginaceae), and Senecio douglasii var. longilobus (Asteraceae).
R A, Copper +3 more
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Separation of rare earth elements by high-speed counter-current chromatography
Journal of Chromatography A, 1991Besides being widely used in electronic and glass industries, rare earth elements have recently been found to have important biological effects including the ability to stabilize and enhance interferon activity [J.J. Sedmak and S.E. Grossberg, J. Gen. Virol, 52 (1981) 195].
E, Kitazume, M, Bhatnagar, Y, Ito
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Evaluation of different tubing geometries for high-speed counter-current chromatography
Journal of Chromatography A, 2001Different tubing geometries were evaluated for use in high-speed counter-current chromatography. Standard round tubing was compared to rectangular and twisted rectangular tubing. The number of theoretical plates for a standard anthocyanin mixture from black currant was determined for each experiment.
A, Degenhardt +3 more
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Separation of alkaloids from herbs using high‐speed counter‐current chromatography
Journal of Separation Science, 2011AbstractAlkaloids represent a most widespread group of bioactive natural products. Because of their alkalinity and structural diversity, the fractionation and purification of the alkaloids from herbs can often present a number of practical difficulties using the conventional chromatographic techniques.
Lei, Fang +4 more
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Separation of lac dye components by high-speed counter-current chromatography
Journal of Chromatography A, 1998High-speed counter-current chromatography has been successfully applied to the separation of the lac dye components. A 25-mg quantity of the sample was separated using a two-phase solvent system composed of tert.-butyl methyl ether-n-butanol-acetonitrile-water (2:2:1:5).
H, Oka +13 more
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