Results 251 to 260 of about 56,741 (293)

Separation of salidroside from Rhodiola crenulata by high-speed counter-current chromatography

Journal of Chromatography A, 2002
High-speed counter-current chromatography (HSCCC) was used to purify salidroside from an extract of Rhodiola crenulata with two steps using a two-phase solvent system composed of ethyl acetate-n-butanol-water (1:4:5, v/v) in the first run and chloroform-methanol-isopropanol-water (5:6:1:4) in the second run.
Xiao, Han, Tianyou, Zhang, Yun, Wei, Xueli, Cao, Yoichiro, Ito   +4 more
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Improved method for continuous uv monitoring in high-speed counter-current chromatography

Journal of Chromatography A, 1989
Continuous UV monitoring of the effluent in high-speed counter-current chromatography often encounters difficulty mainly due to the thermolabile nature of the mobile phase which tends to develop turbidity in the flow cell under a slight shift of the ambient temperature.
H, Oka, Y, Ito
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Separation of Rare Earth Element Radioisotopes by Reverse-Phase High-Speed Counter-Current Chromatography

Journal of Chromatography A, 2023
Analytical scale purification of rare earth element (REE) radioisotopes is typically accomplished using cation-exchange resins (e.g. AG 50W-X8) and high-performance liquid chromatography (HPLC). Despite the variety of improvements made since the development of this separation process in the 1950s, nearest neighbor separations remain a challenge, as ...
Mateusz Dembowski, Hope E. Rasmussen, John E. Rowley, Janelle E. Droessler, George S. Goff, Iain May   +5 more
openaire   +2 more sources

Isolation of bacitracins A and F by high-speed counter-current chromatography

Journal of Chromatography A, 1991
The major components of bacitracin were separated and purified using high-speed counter-current chromatography (HSCCC). A systematic search for optimum two-phase solvent systems resulted in two systems: chloroform-ethanol-methanol-water (5:3:3:4) and chloroform-ethanol-water (5:4:3).
K, Harada, Y, Ikai, Y, Yamazaki, H, Oka, M, Suzuki, H, Nakazawa, Y, Ito   +6 more
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Preparative separation of grape skin polyphenols by high-speed counter-current chromatography

Food Chemistry, 2016
To develop an efficient method for large preparation of various individual polyphenols from white grape skins (Fernão Pires; Vitis vinifera) by preparative high-speed counter-current chromatography (HSCCC) and preparative-HPLC, an optimized preparative HSCCC condition with two-phase solvent system composed of Hex-EtOAc-H2O (1:50:50, v/v) was used to ...
Lanxin, Luo, Yan, Cui, Shuting, Zhang, Lingxi, Li, Yuanyuan, Li, Peiyu, Zhou, Baoshan, Sun   +6 more
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Dual-mode high-speed counter-current chromatography: retention, resolution and examples

Journal of Chromatography A, 1997
Counter-current chromatography (CCC) is a very versatile technique offering high resolution power in recovering very pure compounds from complex matrices. Dual-mode CCC where the phase role is reversed during the separation is investigated here; it ensures elution of all the injected species from the column while, unlike backflush, the separation is ...
M Agnely, D Thiébaut
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High-speed preparative counter-current chromatography with a coil planet centrifuge

Journal of Chromatography A, 1982
Abstract A compact table-top model of a flow-through coil planet centrifuge produces and efficient chromatagraphic separation of solutes on a preparative scale within 2–5 h. The use of a multi-layer coiled column promotes retention of the stationary phase with a high flow-rate of the mobile phase and permits almost universal application of ...
Yoichiro Ito, Jesse Sandlin, William G. Bowers   +2 more
openaire   +1 more source

A review of the hydrodynamics of high-speed counter current chromatography

2023
This review outlines the current understanding of the flow behavior in high-speed counter-current chromatography (HSCCC). HSCCC has seen huge progress in modeling and predicting flow behavior, yet it still is not fully understood from a hydrodynamic perspective.
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Purification of paeoniflorin from Paeonia lactiflora Pall. by high-speed counter-current chromatography

Journal of Chromatography A, 2004
High-speed counter-current chromatography was successfully used for the first time for the preparative separation and purification of paeoniflorin from the Chinese medicinal plant Paeonia lactiflora Pall. using a two-phase solvent system composed of n-butanol-ethyl acetate-water (1:4:5, v/v) in a single run.
Chen, F, Lu, HT, Jiang, Y
openaire   +4 more sources

Isolation of hydroxycinnamoyltartaric acids from grape pomace by high-speed counter-current chromatography

Journal of Chromatography A, 2006
A method for the isolation of caftaric, coutaric and fertaric acids from grape pomace by high-speed counter-current chromatography (HSCCC) was developed. Using a system of hexane/ethyl acetate/methanol/water 3:7:3:7 (v/v/v/v) and 0.5% trifluoroacetic acid (TFA) in the head-to-tail elution mode, the target compounds were separated from co-extracted ...
Thorsten, Maier, Solveigh, Sanzenbacher, Dietmar R, Kammerer, Nicolai, Berardini, Jürgen, Conrad, Uwe, Beifuss, Reinhold, Carle, Andreas, Schieber   +7 more
openaire   +2 more sources