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Isolation of dammarane saponins from Panax notoginseng by high-speed counter-current chromatography

Journal of Chromatography A, 2003
The Chinese phytomedicinal formulation Sanqi Zongdai Pian, traditionally prepared from crude extracts from roots of Panax notoginseng (Araliaceae), contains highly polar dammarane saponins which were separated at a preparative scale using high-speed counter-current chromatography (HSCCC). In each operation, 283 mg methanolic extract of five tablets was
Qizhen, Du   +3 more
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Separation of proanthocyanidins isolated from tea leaves using high-speed counter-current chromatography

Journal of Chromatography A, 2009
The proanthocyanidin extract from tea (Camellia sinensis) leaves was purified for the further study of the biological role of proanthocyanidins in blister blight leaf disease of tea, which is caused by the fungus Exobasidium vexans. An aqueous acetone extract of proanthocyanidins prepared from healthy tea leaves was partially purified using Sephadex LH-
N, Savitri Kumar   +4 more
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Separation and purification of 10-deacetylbaccatin III by high-speed counter-current chromatography

Journal of Chromatography A, 1998
High-speed counter-current chromatography (HSCCC) was used for the separation and purification of 10-deacetylbaccatin III from the needle extract of Chinese yew (Taxus chinensis). The crude needle extract (500 mg/5 ml) was first separated with a two-phase solvent system composed of n-hexane-ethyl acetate-ethanol-water (2:5:2:5, v/v) and the partially ...
X, Cao, Y, Tian, T Y, Zhang, Y, Ito
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Preparative separation of isoflavone components in soybeans using high-speed counter-current chromatography

Journal of Chromatography A, 2001
Four isoflavone components were purified from soybean extract by high-speed counter-current chromatography (HSCCC). Two types of multilayer coil separation columns were used: a small column made of standard 2.6-mm I.D. PTFE (polytetrafluoroethylene) tubing with a 260-ml capacity and a large column of convoluted PTFE tubing of 5.7-mm average I.D. with a
Q, Du, Z, Lib, Y, Ito
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Scaling High-Speed Counter-Current Chromatography for Preparative Neodymium Purification: Insights and Challenges

Journal of Chromatography A
Efficient rare earth element (REE) separations are becoming increasingly important to technologies ranging from renewable energy and high-performance magnets to applied radioisotope separations. These separations are made challenging by the extremely similar chemical and physical characteristics of the individual elements, which almost always occupy ...
Alexander B. Weberg   +5 more
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Laser-light-scattering detection for high-speed countercurrent chromatography

Journal of Chromatography A, 1991
High-speed counter-current chromatography (HSCCC) is a powerful separation technique increasingly used in natural products chemistry. Evaporative laser light-scattering detection (ELSD) represents a universal detection method based on the following principle: the eluate [e.g.
D E, Schaufelberger   +2 more
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Multilayer coil planet centrifuge for analytical highspeed counter-current chromatography

Journal of Chromatography A, 1989
A compact portable model of a high-speed counter-current chromatograph enables efficient analytical separations of microgram sample quantities within 10 min. A series of preliminary experiments was conducted to study retention of the stationary phase of various two-phase solvent systems in short coils with different helical diameters. Under the optimal
H, Oka, F, Oka, Y, Ito
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[Separation of alkaloids in tea by high-speed counter-current chromatography].

Se pu = Chinese journal of chromatography, 2001
Alkaloids extracted from the green tea were separated by high-speed counter-current chromatography. A series of experiments have been performed to investigate effects of different solvent system. A system of CHCl3-CH3OH-NaH2PO4(23 mmol/L) = (4:3:2) was selected, in which the upper phase was used as the stationary phase, and the lower phase as mobile ...
L, Yuan, R, Fu, T, Zhang, J, Deng, X, Li
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Direct coupling of high-speed counter-current chromatography to thin-layer chromatography

Journal of Chromatography A, 1991
B. Diallo   +2 more
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