Results 101 to 110 of about 11,387,151 (316)

High-Throughput Biotinylation of Proteins [PDF]

, 2009
One of the more useful tags for a protein in biochemical experiments is biotin, because of its femtomolar dissociation constant with streptavidin or avidin. Robust methodologies have been developed for other the in vivo addition of a single biotin to recombinant protein or the in vitro enzymatic or chemical addition of biotin to a protein.
Brian K, Kay, Sang, Thai, Veronica V, Volgina   +2 more
openaire   +2 more sources

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

In vitro high throughput screening of compounds for favorable metabolic properties in drug discovery

, 2001
Drug metabolism can have profound effects on the pharmacological and toxicological profile of therapeutic agents. In the pharmaceutical industry, many in vitro techniques are in place or under development to screen and optimize compounds for favorable ...
Masimirembwa, CM, Andersson, TB, Thompson, R   +2 more
core   +1 more source

Bidirectional fano algorithm for high throughput sequential decoding [PDF]

, 2009
Various techniques, such as bidirectional search, have been employed in sequential decoding to reduce the decoding delay. In this paper, a idirectional Fano algorithm (BFA) is proposed, in which a forward decoder (FD) and a backward decoder (BD) search ...
Koçak, T, Taskin Kocak, Morris, KA, Woodward, G, Ran Xu, Kevin Morris, Xu, R, Graeme Woodward, Craig Dolwin   +8 more
core   +1 more source

Screening for molecular glues – Challenges and opportunities

SLAS Discovery
Molecular glues are small molecules, typically smaller than PROTACs, and usually with improved physicochemical properties that aim to stabilise the interaction between two proteins.
Geoffrey A. Holdgate, Catherine Bardelle, Sophia K. Berry, Alice Lanne, Maria Emanuela Cuomo   +4 more
doaj   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Microarray technology as a universal tool for high-throughput analysis of biological systems

, 2006
Over the last years microarray technology has become one of the principal platform technologies for the high-throughput analysis of biological systems.
Jacob, A, Hoheisel, J D, Bartscherer, K, Sobek, J, Angenendt, P   +4 more
core   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Emerging insights into CC and CXC chemokines and their receptors in Mycobacterium tuberculosis infection

FEBS Open Bio, EarlyView.
The dual roles of CC and CXC chemokines in distinguishing active, latent, and subclinical tuberculosis were reviewed, along with an evaluation of their potential as diagnostic biomarkers and therapeutic targets to advance precision medicine in tuberculosis management. The graphical abstract was generated with AI assistance (Gemini 3.0).
Xuying Yin, Dangsheng Xiao, Jiezuan Yang
wiley   +1 more source

Range and throughput enhancement of wireless local area networks using smart sectorised antennas [PDF]

, 2004
At present, wireless local area networks (WLANs) such as HiperLAN2 and 802.11a are being developed and deployed around the world. In this letter, the use of sectorized antennas is considered as a means to improve the physical layer performance of WLANs ...
Nix, AR, Karlsson, P, Doufexi, A, Bull, DR, Armour, SMD   +4 more
core   +1 more source