Results 81 to 90 of about 11,387,151 (316)

Artificial tissue binding models : development and comparative evaluation of high - throughput lipophilicity assays and their use for PET tracer optimization [PDF]

, 2015
The purpose of this thesis was to increase the efficiency of the Positron Emission Tomography (PET) tracer development process. Since many neuroimaging agents fail due to undesirably high non-specific binding (NSB) to brain tissue, we aimed at estimating
Assmus, Frauke
core   +1 more source

Liquid biopsy‐based diagnostic evaluation of hypermethylated CpG sites for ovarian cancer diagnosis

Molecular Oncology, EarlyView.
This schematic outlines the workflow from biomarker identification to duplex MethyLight assay validation for epithelial ovarian cancer diagnosis using cfDNA‐based liquid biopsy. Initial screening of hypermethylated CpG candidates (cg02957270, cg10061138 cg00480298, COL2A1) was performed in tissue using ARMS‐PCR, COBRA, qPCR and image analysis. Selected
Deepa Bisht, Sameer Gupta, Amrita Chaurasia, Manisha Sachan   +3 more
wiley   +1 more source

Epigenetic heterogeneity and plasticity in therapy‐induced tumor states through single‐cell multi‐omics

Molecular Oncology, EarlyView.
Single‐cell multi‐omics reveals epigenetic heterogeneity across therapy‐adaptive tumor states, including quiescent/dormant, drug‐tolerant persister, and EMT‐like phenotypes. By linking regulatory features with state‐associated biomarkers, these approaches inform biomarker‐guided therapeutic strategies for evolving tumors.
Hee Jung Kim, Hwiyeong Lee, Jin Hong, Daechan Park   +3 more
wiley   +1 more source

Patterning cell using Si-stencil for high-throughput assay [PDF]

, 2011
Patterning cell using Si-stencil for high-throughput assayIn this communication, we report a newly developed cell pattering methodology by a silicon-based stencil, which exhibited advantages such as easy handling, reusability, hydrophilic surface and ...
Qin, Jianhua, Longqing Chen, Zhang, Xixiang, WeijiaWen, Vivian Yu, Zhang, Mengying, Yu, Vivian, Mengying Zhang, Xixiang Zhang, Wen, Weijia, Jinbo Wu, Joseph Tin-Yum Wong, Wu, Jinbo, Wong, Joseph Tin-Yum, Chen, Longqing, Jianhua Qin, Tin-Yum Wong, Joseph, Weijia Wen   +17 more
core   +1 more source

A High Throughput Synthetic Workflow For Solid State Reactions

, 2023
High-throughput synthetic methods are well-established for chemistries involving liquid- or vapour-phase reagents and have been harnessed to prepare arrays of inorganic materials.
Luke, Daniels, Michael, Gaultois, Matthew, Rosseinsky, John, Claridge, Luca, Arciero, Christopher, Hampson, Troy, Manning, Moli, Smith, Christopher, Collins   +8 more
core   +1 more source

Beta-Myrcene as a Sedative–Hypnotic Component from Lavender Essential Oil in DL-4-Chlorophenylalanine-Induced-Insomnia Mice

Pharmaceuticals
With the increasing prevalence of insomnia-related diseases, the effective treatment of insomnia has become an important health research topic. Lavender (Lavandula angustifolia Mill.) essential oil (LEO) is a commonly used medicine for the treatment of ...
Luge Chen, Yingwei Liu, Dawei Xu, Na Zhang, Yong Chen, Jin Yang, Lijuan Sun   +6 more
doaj   +1 more source

A novel long non-coding RNA-KAT7 is low expressed in colorectal cancer and acts as a tumor suppressor

Cancer Cell International, 2019
Background The abnormal expression of many long non-coding RNAs (lncRNAs) has been reported in the progression of various tumors. However, the potential biological roles and regulatory mechanisms of long non-coding RNAs in the development of colorectal ...
Qingmei Wang, Rongzhang He, Tan Tan, Jia Li, Zheng Hu, Weihao Luo, Lili Duan, Wenna Luo, Dixian Luo   +8 more
doaj   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Breaking shackles of molecular weight and emission for NIR-II fluorophores by regulating Columb attraction interaction

Nature Communications
The second near-infrared (NIR-II) dyes provide advantages for in vivo imaging, but challenges persist. A primary issue is the lack of practicable strategies to balance emission wavelength and molecular weight, particularly for low-molecular-weight ( 1000 
Miantai Ye, Xiaoyu Wang, Jingwen Zou, Wei Sun, Weijie Chi, Zhiqiang Mao, Zhihong Liu   +6 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source