The power of many: when genetics met yeasts and high‐throughput
ABSTRACT In recent years, complex technological capabilities have evolved, driven by the need to solve complex and integrative biological questions through global analyses. New equipment allows the scaling up and automation of processes which previously were carried out on a very limited scale.
Víctor A. Tallada, Víctor Carranco
wiley +1 more source
Assessment of High Throughput Sequencing Quality of Host DNA Enriched From Faeces: A Case From Captive Tiger. [PDF]
Yang J +15 more
europepmc +1 more source
The ageing holobiont: crosstalk between telomere dynamics, oxidative stress and the gut microbiome
ABSTRACT The gut tissue is at the frontline of early onset of ageing. It exhibits high cell turnover rates and rapid telomere shortening, which can have systemic effects on the developing or senescing organism. We conducted a literature review of studies on the crosstalk between telomere length dynamics, telomerase activity, oxidative stress, and gut ...
Michael L. Pepke +2 more
wiley +1 more source
Evaluation of gene editing in CHO cells using the Cas‐CLOVER system
Abstract Recent advances in gene editing technologies have transformed the genetic engineering of Chinese hamster ovary (CHO) hosts, enabling the development of cell lines with improved stability and productivity. In this study, we employed the programmable nuclease (PN) Cas‐CLOVER to precisely target the Glutamine synthetase (GS) locus in CHO cells. A
Tiffany McLamarrah +13 more
wiley +1 more source
Advances in Quantitative Techniques for Mapping RNA Modifications. [PDF]
Tian L, Vallabhaneni B, Chang YH.
europepmc +1 more source
Recent Advances in Chemical Probing Strategies for RNA Structure Determination in Vivo
This Review illustrates how structural probes combined with advanced sequencing technologies are revolutionizing our ability to map RNA structures and interactions within living cells. It highlights the development of innovative strategies tailored to probe RNA structures in particular biological contexts.
Maryana Yarshova +2 more
wiley +1 more source
High-throughput detection of microeukaryotic parasites in insects using Nanopore sequencing. [PDF]
Bessette E, Edwards S, Williams BAP.
europepmc +1 more source
Development and optimization of T-ARMS PCR assays for detection of lethal haplotypes of <i>TADA2A</i>, <i>UR1B</i>, and <i>PORL1B</i> in pigs in Vietnam. [PDF]
Minh Nhut P, Nguyen NM, Bui APN.
europepmc +1 more source
Abstract Background Racehorses undergo profound physiological changes with training and competition, but current biomarkers inadequately capture the complex molecular dynamics of exercise. This study aimed to identify novel plasma biomarkers of training adaptation and peak load using high‐throughput proteomics.
Jowita Grzędzicka +4 more
wiley +1 more source

