Results 61 to 70 of about 61,294 (275)

Liquid biopsy epigenetics: establishing a molecular profile based on cell‐free DNA

Molecular Oncology, EarlyView.
Cell‐free DNA (cfDNA) fragments in plasma from cancer patients carry epigenetic signatures reflecting their cells of origin. These epigenetic features include DNA methylation, nucleosome modifications, and variations in fragmentation. This review describes the biological properties of each feature and explores optimal strategies for harnessing cfDNA ...
Christoffer Trier Maansson, Anders Lade Nielsen, Boe Sandahl Sorensen   +2 more
wiley   +1 more source

Development of a high-throughput system for phenotyping rice roots traits [PDF]

, 2010
A CIRAD project (Orytage) involving NARES and IRC partners aims at developing an international phenotyping network for rice adaptations to drought and thermal stresses in the context of gene discovery and genetic mapping.
Audebert, Alain, Fleuriot, Jean-Paul, Ghneim, Thaura, Roques, Sandrine, Thaunay, Patrice   +4 more
core  

Quantitative insertion-site sequencing (QIseq) for high throughput phenotyping of transposon mutants [PDF]

, 2016
Genetic screening using random transposon insertions has been a powerful tool for uncovering biology in prokaryotes, where whole-genome saturating screens have been performed in multiple organisms. In eukaryotes, such screens have proven more problematic,
Adams, John H., Bronner, Iraad F., Jiang, Rays H. Y., Otto, Thomas D., Quail, Michael A., Rayner, Julian C., Udenze, Kenneth, Wang, Chengqi, Zhang, Min   +8 more
core   +1 more source

High throughput phenotyping for aphid resistance in large plant collections

Plant Methods, 2012
Background Phloem-feeding insects are among the most devastating pests worldwide. They not only cause damage by feeding from the phloem, thereby depleting the plant from photo-assimilates, but also by vectoring viruses. Until now, the main way to prevent
Chen Xi, Vosman Ben, Visser Richard GF, van der Vlugt René AA, Broekgaarden Colette   +4 more
doaj   +1 more source

Wheat rusts never sleep but neither do sequencers: will pathogenomics transform the way plant diseases are managed? [PDF]

, 2015
Field pathogenomics adds highly informative data to surveillance surveys by enabling rapid evaluation of pathogen variability, population structure and host ...
Derevnina, Lida, Michelmore, Richard W
core   +2 more sources

In vitro models of cancer‐associated fibroblast heterogeneity uncover subtype‐specific effects of CRISPR perturbations

Molecular Oncology, EarlyView.
Development of therapies targeting cancer‐associated fibroblasts (CAFs) necessitates preclinical model systems that faithfully represent CAF–tumor biology. We established an in vitro coculture system of patient‐derived pancreatic CAFs and tumor cell lines and demonstrated its recapitulation of primary CAF–tumor biology with single‐cell transcriptomics ...
Elysia Saputra, Shamsudheen Karuthedath Vellarikkal, Lixia Li, Hong Sun, Khoa Nguyen, Amber Montano, Suchitra Natarajan, Federica Piccioni, Alex Michael Tamburino, Xin Yu, Aleksandra Katarzyna Olow   +10 more
wiley   +1 more source

High-throughput phenotyping for trait detection in vineyards

BIO Web of Conferences, 2015
Recently several papers appeared describing initial steps for novel phenotyping technologies in grapevine management, research and breeding. Kicherer and coworkers were the first using a robotic device which permits to follow a GPS track, stopping in the
Kicherer Anna, Herzog Katja, Töpfer Reinhard   +2 more
doaj   +1 more source

High-Throughput System for the Early Quantification of Major Architectural Traits in Olive Breeding Trials Using UAV Images and OBIA Techniques [PDF]

, 2019
The need for the olive farm modernization have encouraged the research of more efficient crop management strategies through cross-breeding programs to release new olive cultivars more suitable for mechanization and use in intensive orchards, with high ...
Casanova Lerma, Laura, Castro Mejías, Ana Isabel de, Jiménez Brenes, Francisco M., Jiménez, María Rocío, López Granados, Francisca, Morales Sillero, Ana María, Rallo Morillo, Pilar, Suárez García, María Paz, Torres Sánchez, Jorge   +8 more
core   +2 more sources

Cis‐regulatory and long noncoding RNA alterations in breast cancer – current insights, biomarker utility, and the critical need for functional validation

Molecular Oncology, EarlyView.
The noncoding region of the genome plays a key role in regulating gene expression, and mutations within these regions are capable of altering it. Researchers have identified multiple functional noncoding mutations associated with increased cancer risk in the genome of breast cancer patients.
Arnau Cuy Saqués, Aracele Martinez‐Mendez, John Crown, Alex Eustace   +3 more
wiley   +1 more source

Transcriptional network analysis of PTEN‐protein‐deficient prostate tumors reveals robust stromal reprogramming and signs of senescent paracrine communication

Molecular Oncology, EarlyView.
Combining PTEN protein assessment and transcriptomic profiling of prostate tumors, we uncovered a network enriched in senescence and extracellular matrix (ECM) programs associated with PTEN loss and conserved in a mouse model. We show that PTEN‐deficient cells trigger paracrine remodeling of the surrounding stroma and this information could help ...
Ivana Rondon‐Lorefice, Jose I. Lopez, Aitziber Ugalde‐Olano, Maite Zufiaurre, Ianire Astobiza, Natalia Martin‐Martin, Laura Bozal‐Basterra, Saioa Garcia‐Longarte, Amaia Zabala‐Letona, Sofia Rey, Aida Santos‐Martin, Miguel Unda, Ana Loizaga‐Iriarte, Mariona Graupera, Paolo Nuciforo, Arkaitz Carracedo, Isabel Mendizabal   +16 more
wiley   +1 more source